中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 51-55.doi: 10.3969/j.issn.1673-8225.2012.01.011

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

小鼠脂肪源干细胞分离培养和成骨诱导前后造血调控因子的表达**★

郝  丹1，段显琳1，毕晓娟2，常相萍1，马  艳2，曲建华1，袁海龙1，江  明1，2   

  1. 1新疆血液病研究所、新疆医科大学第一附属医院血液二科；新疆维吾尔自治区乌鲁木齐市  830054；  2新疆医科大学临床医学研究院、新疆医科大学第一附属医院干细胞研究中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2011-09-16 修回日期:2011-10-24 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 江明，主任医师，教授，硕士生导师，新疆血液病研究所、新疆医科大学第一附属医院血液二科，新疆维吾尔自治区乌鲁木齐市 830054 jiangmingyy@yahoo.com.cn
  • 作者简介:郝丹★，女，1982年生，山西省忻州市人，汉族，新疆医科大学在读硕士，主要从事造血干细胞移植方向的研究。 haodan2000@163.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金项目(200821113)；新疆医科大学第一附属医院干细胞专项基金(2010GXB02)。

Isolation and culture of mouse adipose-derived stem cells and the expression of hematopoietic modulation factors before and after osteogenic induction

Hao Dan1, Duan Xian-lin1, Bi Xiao-juan2, Chang Xiang-ping1, Ma Yan2, Qu Jian-hua1, Yuan Hai-long1, Jiang Ming1, 2   

  1. 1Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 2Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-09-16 Revised:2011-10-24 Online:2012-01-01 Published:2012-01-01
  • Contact: Jiang Ming, Master’ s supervisor, Professor, Chief physician, Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Stem Cell Laboratory, Clinical Medicine Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, Chinajiangmingyy@yahoo.com.cn
  • About author:Hao Dan★, Studying for master’s degree, Second Department of Hematology, Institute of Hematology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China haodan2000@163.com
  • Supported by:
    the Natural Scinence Foundation of Xinjiang Uygur Autonomous Region, No. 200821113*; Special Foundation of Stem Cells, the First Affiliated Hospital of Xinjiang Medical University, No. 2010GXB02*
     

PDF

392

摘要：

背景：小鼠脂肪源干细胞的体外分离培养和诱导分化以及成骨诱导前后造血调控因子表达的报道甚少。
目的：观察小鼠脂肪源干细胞的体外分离培养方法以及成骨诱导前后造血调控因子分子水平的表达。
方法：采用酶消化法获得雄性C57BL/6小鼠脂肪来源的细胞，传代培养至第3代，分别进行成脂与成骨细胞诱导。
结果与结论：小鼠脂肪来源的细胞体外培养呈梭形贴壁生长，可稳定传代。细胞表面标志CD29表达率强阳性，CD34表达率较低，CD106、CD49d低表达。成脂及成骨细胞诱导均为阳性。造血调控因子β-连环蛋白表达量最高，基质细胞衍生因子1和神经钙黏素次之，其余干细胞因子、巨噬细胞炎性蛋白1α、配体jagged1、粒细胞-巨噬细胞集落刺激因子均为低表达。结果证实，小鼠脂肪来源的细胞为脂肪源干细胞，其成骨诱导前后的造血调控因子均有表达，但差异无显著意义。
关键词：造血调控因子；脂肪源干细胞；成骨细胞；诱导；小鼠
doi:10.3969/j.issn.1673-8225.2012.01.011

关键词: 造血调控因子, 脂肪源干细胞, 成骨细胞, 诱导, 小鼠

Abstract:

BACKGROUND: There are few reports about the isolation and culture of mouse adipose-derived stem cells in vitro and the expression of hematopoietic modulation factors before and after osteogenic induction.
OBJECTIVE: To investigate the isolation and culture of mouse adipose-derived stem cells (ADSCs) and molecule level of hematopoietic modulation factors before and after osteogenic induction in vitro.
METHODS: ADSCs were isolated from male C57BL/6 mouse by digesting adipose tissue with collagen typeⅠ, and expanded to three passages after primary culture in a control medium. Then the cells underwent the adipogenic and osteogenic induction.
RESULTS AND CONCLUSION: ADSCs grew as spindle-shaped adherent cells when cultured in vitro, and could stably proliferate and be passed. The cells’ surface antigen phenotype was analyzed by flow cytometry, and the expression of CD29 was strongly positive, CD34 was lower, the expression of CD106 and CD49d was negative. ADSCs incubated in the adipogenic medium and osteogenic medium were stained positively. The expression of hematopoietic modulation factors-catenin was the highest, the stromal cell derived factor-1 and N-cadherin was followed by, the expression of the other factors such as stem cell factor, macrophage inflammatory protein-1α, ligand jagged1 and granulocyte macrophage colony-stimulating factor were all lower. It indicates that the cells from adipose tissue are ADSCs, and the expression of hematopoietic modulation factors is found before and after osteogenic induction, but has no significant difference. 

中图分类号: