中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 22-26.doi: 10.3969/j.issn.1673-8225.2012.01.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞条件培养液对H2O2损伤大鼠心肌细胞的保护★

刘新宾1，2，张红超2，郭子宽3   

  1. 河北北方学院，河北省张家口市   075000；2解放军空军总医院心血管外科，北京市   100142；3军事医学科学院放射与辐射医学研究所实验血液学研究室，北京市
    100850
  • 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 通讯作者：张红超，博士，副主任医师，解放军空军总医院心血管外科，北京市100142 zhanghc2002@gmail.com
  • 作者简介:刘新宾★，男，1982年，山东省曹县人，汉族，河北北方学院在读硕士，医师，主要从事心肌保护的研究。lxblp1237@126.com

Effect of mesenchymal stem cells conditioned medium on protection of hydrogen peroxide injured cardiomyocytes in rats

Liu Xin-bin1, 2, Zhang Hong-chao2, Guo Zi-kuan3   

  1. 1Hebei North University, Zhangjiakou  075000, Hebei Province, China; 2Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing  100142, China; 3Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing  100850, China
  • Online:2012-01-01 Published:2012-01-01
  • Contact: Correspondence to: Zhang Hong-chao, Doctor, Associate chief physician, Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing 100142, Chinazhanghc2002@gmail.com
  • About author:Liu Xin-bin★, Studying for master’s degree, Physician, Hebei North University, Zhangjiakou 075000, Hebei Province, China; Department of Cardiovascular Surgery, Airforce General Hospital of Chinese PLA, Beijing 100142, China lxblp1237@126.com

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摘要：

背景：间充质干细胞移植可以降低心肌梗死面积、改善心功能，目前多数认为间充质干细胞分泌生物因子起主要作用。
目的：观察骨髓间充质干细胞条件培养液对过氧化氢诱导新生大鼠心肌细胞凋亡的影响。
方法：分离培养并鉴定骨髓间充质干细胞和新生大鼠心肌细胞，制作骨髓间充质干细胞条件培养液以及过氧化氢损伤新生大鼠心肌细胞模型，实验分为4组：对照组、模型组、骨髓间充质干细胞条件培养液组以及骨髓间充质干细胞条件培养液+ PI3K 抑制剂处理组。以ELISA检测乳酸脱氢酶活性和流式细胞术检测细胞凋亡率来评估新生大鼠心肌细胞损伤程度。
结果与结论：模型组乳酸脱氢酶活性明显高于其余各组(P < 0.01)，同时骨髓间充质干细胞条件培养液+抑制剂组也高于骨髓间充质干细胞条件培养液组(P < 0.01)；骨髓间充质干细胞条件培养液组细胞凋亡率低于与骨髓间充质干细胞条件培养液+抑制剂组(P < 0.01)，并且都低于与模型组(P < 0.01)。证明了骨髓间充质干细胞条件培养液抑制了过氧化氢诱导的新生大鼠心肌细胞凋亡，该作用部分是通过PI3K途径发挥的。
关键词：心肌细胞；骨髓间充质干细胞；细胞凋亡；缺血再灌注；过氧化氢

关键词: 心肌细胞, 骨髓间充质干细胞, 细胞凋亡, 缺血再灌注, 过氧化氢

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) transplantation can reduce myocardial infarct area and improve cardiac function. To date, most believe that MSCs play an important role in secreting biological factors.
OBJECTIVE: To observe the influence of MSCs conditioned medium (MSCs-CM) on cardiomyocytes apoptosis induced by hydrogen peroxide (H2O2).
METHODS: Rat MSCs and cardiomyocytes were isolated, cultured separately and identified. Preparation of MSCs-CM and H2O2 injured NRCs model. The rats were divided into four groups: control group, model group, MSCs-CM group and MSCs-CM+inhibitors of PI3K (Wortmannin) group. Lactate dehydrogenase (LDH) activity and apoptosis rates were detected by ELISA and flow cytometry separately in order to assess cardiomyocytes injury.
RESULTS AND CONCLUSIONS: LDH activity in the model group was significantly higher than that in the other groups (P < 0.001); While the LDH activity in the MSCs-CM+Wortmannin group was also higher than that in the MSCs-CM group (P < 0.001). The apoptosis rate in the MSCs-CM group was lower than that in the MSCs-CM+ Wortmannin group (P < 0.01), and the apoptosis rate in the two former groups was lower than that in the model group (P < 0.01). It indicated that MSC-CM inhibited H2O2-induced apoptosis of NRCs partially via PI3K pathway.

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