中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9385-9388.doi: 10.3969/j.issn.1673-8225.2011.50.019

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

兔桡骨临界骨缺损模型的制备

周  芳1，李  静1，余  磊2，廖  华2，欧阳钧2   

  1. 1南方医科大学，广东省广州市   510515
    2南方医科大学人体解剖学教研室，广东省组织构建与检测重点实验室，广东省广州市510515
  • 收稿日期:2011-05-27 修回日期:2011-07-12 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 廖华，博士，教授，南方医科大学人体解剖学教研室，广东省组织构建与检测重点实验室，广东省广州市 510515 并列通讯作者：欧阳钧，博士，教授，南方医科大学人体解剖学教研室，广东省组织构建与检测重点实验室，广东省广州市 510515 jouyang@126.com
  • 作者简介:周芳，女，1987年生，湖北省建始县人，土家族，南方医科大学2006级临床医学（本硕博连读）八年制在读。 并列第一作者：李静，女，1988年生，河北省玉田县人，汉族，南方医科大学2006级临床医学(本硕博连读)八年制在读。 Liyu12399@126.com
  • 基金资助:

    国家高技术研究发展计划(863计划)(2006AA02A118)。

Establishment of a radial bone critical defect model in a rabbit

Zhou Fang1, Li Jing1, Yu Lei2, Liao Hua2, Ouyang Jun2   

  1. 1Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China
  • Received:2011-05-27 Revised:2011-07-12 Online:2011-12-10 Published:2011-12-10
  • Contact: Liao Hua, Doctor, Professor, Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China Correspondence to: Ouyang Jun, Doctor, Professor, Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China jouyang@126.com
  • About author:Zhou Fang☆, Studying for doctorate, Southern Medical University, Guangzhou 510515, Guangdong Province, China Li Jing, Studying for doctorate, Southern Medical University, Guangzhou 510515, Guangdong Province, China Liyu12399@126.com Zhou Fang and Li Jing contributed equally to this paper.
  • Supported by:

    the National High Technology Research and Development Program of China (863 Project), No. 2006AA02A118*

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摘要：

背景：各种原因造成的骨折和骨缺损导致的骨不连一直是临床骨科修复的一大难题，相关产品质量的检验需要标准的骨缺损模型，然而骨缺损的临界长度至今仍无定论。
目的：建立兔桡骨骨缺损模型，以确定兔桡骨临界骨缺损长度。
方法：将18只雄性新西兰大白兔，随机分为6组，在双侧桡骨中段分别做12，13，14，15，16，17 mm的缺损，伤口缝合包扎但不固定。
结果与结论：大体标本和放射学观察显示，3个月内12，13，14，15，16 mm组的缺损均有修复完整的情况，但17 mm组无一例修复。组织学结果显示12，13，14，15，16 mm组缺损修复区有骨小梁与骨基质的形成、骨再生和再血管化、髓腔不同程度再通以及成骨细胞，17 mm组可见成骨细胞，破骨细胞，但未见骨再血管化和髓腔再通。故兔桡骨临界骨缺损长度为17 mm。

关键词: 桡骨, 骨缺损, 临界骨缺损, 长度, 兔

Abstract:

BACKGROUND: Nonunion caused by various reasons of fractures and bone defects has been a major problem of clinical orthopedic rehabilitation. With the rapid development of tissue engineering, product quality testing needs standard bone defect model. However, the length of bone critical-size defects has not been ascertained.
OBJECTIVE: To establish rabbit radial bone defect models and to determine the length of rabbit radial bone critical-size defects.
METHODS: Eighteen male New Zealand white rabbits (2.0-2.5 kg) were randomly assigned into 6 groups and then 12, 13, 14, 15, 16 and 17 mm segmental defects were created in the middle part of radius on both sides, respectively. The incisions were sutured and bandaged, but not fixed.
RESULTS AND CONCLUSION: According to examination with naked eyes and X-ray, some of the segmental defects with the length of 12, 13, 14, 15 and 16 mm were completely repaired. But none of the defects with the length of 17 mm was repaired. Histological examination showed that there was trabecular bone formation, bone matrix, medullary cavity recanalization, revascularization and osteoblasts in the segmental defects with the length of 12, 13, 14, 15 and 16 mm. However, there were osteoblasts, osteoclasts in the 17 mm defects, but no medullary cavity recanalization or revascularization. Therefore, the length of bone critical-size defect in rabbit radius was 17 mm.

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