中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8851-8854.doi: 10.3969/j.issn.1673-8225.2011.47.027

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

改性纳米羟基磷灰石表面性质及对成骨细胞贴壁生长的影响

魏俊超1，陈  磊2，陈义旺1，陈学思2   

  1. 1南昌大学化学系，江西省南昌市 330031
    2中科院长春应用化学研究所生态环境高分子重点实验室，吉林省长春市 130022
  • 收稿日期:2011-09-07 修回日期:2011-10-08 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 陈义旺，博士，教授，南昌大学化学系，江西省南昌市 330031 ywchen@ ncu.edu.cn
  • 作者简介:魏俊超☆，男，1981年生，河南省许昌市人。博士，讲师，主要从事生物医用高分子材料研究。

Effect of surface properties of modified hydroxyapatite on the adherent growth of osteoblasts

Wei Jun-chao1, Chen Lei2, Chen Yi-wang1, Chen Xue-si2   

  1. 1Department of Chemistry, Nanchang University, Nanchang  330031, Jiangxi Province, China
    2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun  130022, Jilin Province, China
  • Received:2011-09-07 Revised:2011-10-08 Online:2011-11-19 Published:2011-11-19
  • Contact: Chen Yi-wang, Doctor, Professor, Department of Chemistry, Nanchang University, Nanchang 330031, Jiangxi Province, China ywchen@ncu.edu.cn
  • About author:Wei Jun-chao☆, Doctor, Lecturer, Department of Chemistry, Nanchang University, Nanchang 330031, Jiangxi Province, China

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438

被引次数

3

摘要：

背景：生物材料表面性质的改变能够影响其与细胞的相互作用，并进一步影响细胞黏附能力、基因表达等行为。
目的：评价改性纳米羟基磷灰石的表面性质及其对成骨细胞在材料表面黏附能力的影响。
方法：参考作者既往实验制备纳米羟基磷灰石和改性纳米羟基磷灰石，利用zeta电位仪测定改性纳米羟基磷灰石的zeta电位，通过测定水在改性羟基磷灰石表面的接触角，结合成骨细胞黏附试验，分析表面改性对材料的细胞黏附能力和生物相容性的影响。
结果与结论：改性纳米羟基磷灰石表面呈疏水性，接触角为93°，成骨细胞黏附实验表明，改性纳米羟基磷灰石表面比纳米羟基磷灰石表面黏附更多的成骨细胞，表明表面改性增强了纳米羟基磷灰石的生物相容性。

关键词: 羟基磷灰石, 改性纳米羟基磷灰石, 骨组织工程, 表面改性, 生物材料

Abstract:

BACKGROUND: The alteration of biomaterial surface properties may affect interaction between the biomaterials and cells, which will elicit diverse cell behaviors, such as cell adhesion, gene expression and so on.
OBJECTIVE: To investigate the effects of surface properties of a new type of polymer modified hydroxyapatite (HA) and to determine its effects on adhesion ability of osteoblasts.
METHODS: HA and modified HA were prepared according to author’s previous experiments. The zeta potential of modified HA was measured by using zeta potential analyzer. The water contact angle and cell attachment test were carried out to investigate the effect of surface modification on the cell adhesion ability and the biocompatibility of modified HA.
RESULTS AND CONCLUSION: The surface of modified HA was hydrophobic, and its water contact angle was 93°. Cell attachment test showed that the number of osteoblasts attached on the modified HA surface was higher than that on the HA surface. It is indicated that the surface modification enhances the biocompatibility of HA.

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