中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8847-8850.doi: 10.3969/j.issn.1673-8225.2011.47.026

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

阿苯达唑壳聚糖纳米粒包封率的测定

连文元1，何承辉2，邢建国2，王新春1,3，黄川生3   

  1. 1石河子大学药学院，新疆维吾尔自治区石河子市  832002
    2新疆维吾尔自治区药物研究所，新疆维吾尔自治区乌鲁木齐市   830004
    3石河子大学医学院第一附属医院，新疆维吾尔自治区石河子市  832008
  • 收稿日期:2011-05-27 修回日期:2011-08-03 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 王新春，博士，主任药师，硕士生导师，石河子大学药学院，新疆维吾尔自治区石河子市 832002；石河子大学医学院第一附属医院，新疆维吾尔自治区石河子市 832008 cwjwxc@ 163.com
  • 作者简介:连文元★，男，1987年生，福建省泉州市人，汉族，石河子大学在读硕士，主要从事中西药物新制剂研究。
  • 基金资助:

    新疆维吾尔自治区青年基金专项(2009211B36)。

Determination of entrapment efficiency of albendazole chitosan nanoparticles

Lian Wen-yuan1, He Cheng-hui2, Xing Jian-guo2, Wang Xin-chun1,3, Huang Chuan-sheng3   

  1. 1School of Pharmacy, Shihezi University, Shihezi  832000, Xinjiang Uygur Autonomous Region, China
    2Xinjiang Institute of Materia Medica, Urumqi  830004, Xinjiang Uygur Autonomous Region, China
    3the First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China
  • Received:2011-05-27 Revised:2011-08-03 Online:2011-11-19 Published:2011-11-19
  • Contact: Wang Xin-chun, Doctor, Chief pharmacist, Master’s supervisor, School of Pharmacy, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China; the First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China cwjwxc@163.com
  • About author:Lian Wen-yuan★, Studying for master’s degree, School of Pharmacy, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the Youth Special Fund of Xinjiang Uygur Autonomous Region, No. 2009211B36*

PDF

340

被引次数

5

摘要：

背景：在纳米粒研究过程中，包封率是纳米粒质量评定的重要指标。
目的：建立阿苯达唑壳聚糖纳米粒包封率的测定方法。
方法：采用紫外分光光度法测定阿苯达唑的含量，反相透析法分离纳米粒和游离药物阿苯达唑，测定阿苯达唑的包封率。考察药物加样回收率、透析平衡时间和透析体积比。
结果与结论：反相透析法能将纳米粒和游离药物阿苯达唑有效分离，药物加样回收率是(99.59±0.36)%，透析法平衡时间为 9 h，透析体积比为1∶15，阿苯达唑壳聚糖纳米粒的平均包封率为(51.73±0.18)%。提示反透析法便捷、准确，适用于阿苯达唑壳聚糖纳米粒包封率的测定。

关键词: 阿苯达唑, 壳聚糖纳米粒, 包封率, 反相透析法, 透析体积比

Abstract:

BACKGROUND: In the course of studying nanoparticles, the entrapment efficiency is a very important index of nanoparticle quality evaluation.
OBJECTIVE: To establish a method for determination of the entrapment efficiency of albendazole chitosan nanoparticles.
METHODS: Reverse dialysis method was used to determine the entrapment efficiency of albendazole chitosan nanoparticles by analyzing the amount of albendazole with ultraviolet spectrophotometry.
RESULTS AND CONCLUSION: The reverse dialysis method could separate albendazole chitosan nanoparticles from free albendazole, its equilibrium time and the dialyzed volume ratio was 9 hours and 1:15, respectively. The sample recovery rate was (99.59±0.36)%. The average entrapment efficiency of albendazole chitosan nanoparticles was (51.73±0.18)%. Reverse dialysis method was convenient and accurate. It was suitable for determining the entrapment efficiency of albendazole chitosan nanoparticles.

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