中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8745-8748.doi: 10.3969/j.issn.1673-8225.2011.47.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

数字化载银羟基磷灰石人工骨抗菌性及生物相容性

朱  辉1,2，尹庆水2，张  余2，张  宇2，赵华福2，周  烨2   

  1. 1南方医科大学研究生学院，广东省广州市510515
    2解放军广州军区广州总医院骨科医院，广东省广州市510010
  • 收稿日期:2011-08-17 修回日期:2011-09-30 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 尹庆水，博士生导师，教授，解放军广州军区广州总医院骨科医院，广东省广州市510010 gz_yqs@126.com
  • 作者简介:朱辉☆，男，1979年生，江西省南丰县人，汉族，南方医科大学在读博士，医师，主要从事脊柱外科及骨组织工程方面研究。 zhuhui04@126.com
  • 基金资助:

    全军医学科学技术研究“十一五”计划第二批课题专项(08Z010)。

Antibacterial property and biocompatibility of digital silver loaded hydroxyapatite as artificial bone

Zhu Hui1,2, Yin Qing-shui2, Zhang Yu2, Zhang Yu2, Zhao Hua-fu2, Zhou Ye2   

  1. 1Graduate School of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2011-08-17 Revised:2011-09-30 Online:2011-11-19 Published:2011-11-19
  • Contact: Yin Qing-shui, Doctoral supervisor, Professor, Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China gz_yqs@126.com
  • About author:Zhu Hui☆, Studying for doctorate, Physician, Graduate School of Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Orthopedics, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China zhuhui04@126.com
  • Supported by:

    Military Research Project of Science and Technology during the Eleventh Five-Year Plan Period, Second Group Topic Special Purpose, No. 08Z010*

PDF

450

被引次数

17

摘要：

背景：载银珊瑚羟基磷灰石作为一种新型抗菌植骨材料，受到越来越多的关注，作为植入物需与人体具有良好的生物相容性。
目的：观察数字化载银珊瑚羟基磷灰石人工骨材料的抗菌性及生物相容性。
方法：将珊瑚羟基磷灰石粉末浸泡于不同浓度的硝酸银溶液，制备出不同含银量的载银羟基磷灰石，再将其与聚乳酸混合，并通过选择性激光烧结快速成形制备出具有特殊形状的数字化载银抗菌人工骨材料。
结果与结论：连续光源原子吸收光谱仪测定珊瑚羟基磷灰石浸泡于10-2，10-3，10-4，10-5 mol/L AgNO3中制备的载银人工骨中Ag+的含量分别为2.31×10-1%，3.18×10-2%，6.75×10-3%，6.05×10-4%。体外抑菌圈实验表明浸泡10-2 mol/L AgNO3中制备的载银人工骨材料对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为(13.00±1.52)mm 及(12.30±1.65)mm；浸泡10-3 mol/L AgNO3中制备的载银人工骨材料分别为(11.50±0.73) mm及(11.00±0.46) mm。浸泡10-4，10-5 mol/L AgNO3载银人工骨材料对两种细菌均无抑菌圈产生。细胞毒性试验结果表明100%浸泡10-2，10-3，10-4，10-5 mol/L AgNO3的载银珊瑚羟基磷灰石人工骨材料的细胞毒性分别为3级、1级、0级、0级。急性全身毒性试验表明浸泡10-3 mol/L AgNO3中的人工骨浸提液对小鼠无明显的急性毒性反应，具有良好的安全性。结果表明浸泡10-3 mol/L AgNO3中的载银珊瑚羟基磷灰石人工骨在体外对金黄色葡萄球菌及大肠杆菌有明显抗菌作用，且具有良好的生物相容性及安全性。

关键词: 珊瑚羟基磷灰石, 载银, 抗菌作用, 生物相容性, 安全性

Abstract:

BACKGROUND: The silver loaded coral hydroxyapatite (CHA-Ag) has received increasing attention as a new type of antibacterial bone graft material. Implant materials need well biocompatibility with human body.
OBJECTIVE: To observe the antibacterial property and biocompatibility of digital CHA-Ag as artificial bone.
METHODS: The coral hydroxyapatite powder was immersed in silver nitrate solution (AgNO3) with different concentrations in order to prepare CHA-Ag containing different contents of Ag+. Then the prepared CHA-Ag was mixed with poly lactic acid and burned by selective laser to prepare digital artificial bone material containing silver with special shape rapidly.
RESULTS AND CONCLUSION: The continuum source atomic absorption spectrometer showed that the Ag+ contents of CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3 were 2.31×10-1%, 3.18×10-2%, 6.75×10-3%, and 6.05×10-4% respectively. The inhibition ring test demonstrated that the inhibition ring diameter of CHA-Ag by 10-2 mol/L AgNO3 against staphylococcus aureus and Escherichia coli were (13.00±1.52) mm and (12.30 ±1.65) mm respectively; while that of 10-3 mol/L were (11.50±0.73) mm and (11.00±0.4) mm respectively, and no inhibition ring occurred in that of 10-4 and 10-5 mol/L AgNO3. Cytotoxicity test manifested that the cytotoxicity grades were 3, 1, 0 and 0 respectively according to the CHA-Ag prepared with 10-2, 10-3, 10-4 and 10-5 mol/L AgNO3. General toxicity test indicated that the artificial bone leaching liquor with 10-3 mol/L AgNO3 had no obvious acute cytotoxicity in mice. The CHA-Ag prepared with 10-3 mol/L AgNO3 has good biocompatibility and biological security, can inhibit the growth of staphylococcus aureus and Escherichia coli in vitro.

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