中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8499-8502.doi: 10.3969/j.issn.1673-8225.2011.45.031

• 干细胞转基因表达 transgenic expression in stem cells • 上一篇    下一篇

异基因间充质干细胞在体内免疫调节作用的时间及剂量依赖性

朱希山，施  薇，台卫平，张  舸，安广宇   

  1. 首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)肿瘤内科，北京市  100038
  • 收稿日期:2011-05-02 修回日期:2011-09-22 出版日期:2011-11-05 发布日期:2011-11-05
  • 作者简介:朱希山☆，男，山东省潍坊市人，汉族，2009年北京协和医学院(清华大学医学部)毕业，博士，医师，主要从事肿瘤干细胞基础研究和临床应用的研究。 mountain.red@163.com

Time- and dose-dependent immunomodulatory effect of allogeneic mesenchymal stem cells in vivo

Zhu Xi-shan, Shi Wei, Tai Wei-ping, Zhang Ge, An Guang-yu   

  1. Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing  100038, China
  • Received:2011-05-02 Revised:2011-09-22 Online:2011-11-05 Published:2011-11-05
  • About author:Zhu Xi-shan☆, Doctor, Physician, Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China mountain.red@163.com

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被引次数

1

摘要：

背景：异基因间充质干细胞移植时，发挥作用需要的剂量、持续的时间及是否具有不良反应是目前研究的热点。
目的：观察异基因的骨髓间充质干细胞在体内免疫调节作用的时间、剂量依赖性。
方法：免疫系统正常的BALB/c小鼠模型，制备骨髓来源间充质干细胞，24只BALB/c小鼠随机分为4组，实验组每只小鼠分别通过尾静脉注射0.3 mL细胞悬液，分别含5×105，5×104，5×103骨髓间充质干细胞，对照组小鼠仅注射0.3 mL生理盐水。进行淋巴细胞增殖检测、混合淋巴细胞反应、间充质干细胞移植对异基因小鼠免疫系统的影响等实验。
结果与结论：不同剂量的C57BL/6骨髓来源间充质干细胞经尾静脉注射给BALB/c受体小鼠后，骨髓间充质干细胞体内对免疫系统的调节呈剂量依赖性。间充质干细胞促进异基因移植物的植入，同时这种免疫下调作用在2周时最强，1个月逐渐减弱，2个月基本消失，提示间充质干细胞的免疫调节作用具有剂量依赖性，并且只能维持一段时间。

关键词: 间充质干细胞, 异基因, 免疫调节, 剂量, 骨髓

Abstract:

BACKGROUND: Allogeneic mesenchymal stem cells transplantation is widely used in clinics, but the required dose, duration and whether the side effects exists are still a research hotspot.
OBJECTIVE: To study the time- and dose-dependent immunomodulatory effect of allogeneic bone marrow mesenchymal stem cells in vivo. 
METHODS: Bone marrow-derived mesenchymal stem cells were isolated from mice with normal immune system. Twenty-four BALB/c mice were randomly divided into four groups. In experimental group, each mouse was injected with 0.3 mL cells suspension containing 5×105, 5×104, 5×103 bone marrow-derived mesenchymal stem cells via the tail vein, respectively. In control group, the mice were only injected with 0.3 mL normal saline. The lymphocyte proliferation test, mixed lymphocyte reaction and experiments about the effect of bone marrow-derived mesenchymal stem cells transplantation on the immune system of allogeneic mice were performed and detected.
RESULTS AND CONCLUSION: After cell transplantation, bone marrow-derived mesenchymal stem cells exerted the in vivo immunomodulatory effect in a dose-dependent manner. Mesenchymal stem cells could promote the allogeneic graft implantation, and the immunomodulatory effect reached the peak at the 2nd week, gradually decreased at the 1st month and disappeared at the 2nd month. It is indicated that the immunomodulatory effects of mesenchymal stem cells are presented in a dose-dependent manner, which can only be maintained for some time.

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