中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8455-8458.doi: 10.3969/j.issn.1673-8225.2011.45.021

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓基质细胞移植缺血性脑梗死大鼠后神经营养因子的表达

边  颖，孙  峰，何志义   

  1. 沈阳市第五人民医院神经内一科，辽宁省沈阳市 110023
  • 收稿日期:2011-05-05 修回日期:2011-06-13 出版日期:2011-11-05 发布日期:2011-11-05
  • 作者简介:边颖★，女，1972年生，辽宁省沈阳市人，汉族，2005年中国医科大学毕业，硕士，副主任医师，主要从事脑血管病的发病机制研究及治疗。 Bianying1001@126.com

Effect of bone marrow stromal cells on neurotrophic factors of rats with ischemic cerebral infarction

Bian Ying, Sun Feng, He Zhi-yi   

  1. First Department of Neurology, the Fifth People’s Hospital of Shenyang, Shenyang  110023, Liaoning Province, China
  • Received:2011-05-05 Revised:2011-06-13 Online:2011-11-05 Published:2011-11-05
  • About author:Bian Ying★, Master, Associate chief physician, First Department of Neurology, the Fifth People’s Hospital of Shenyang, Shenyang 110023, Liaoning Province, China Bianying1001@126.com

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摘要：

背景：骨髓基质细胞在适宜条件下可分化为神经元和星形胶质细胞，分泌可溶性分子促进神经元存活。
目的：观察骨髓基质细胞移植后缺血性脑梗死大鼠脑组织神经营养因子表达情况及其对神经功能的影响。
方法：改良的Longa栓线法制作大鼠大脑中动脉缺血模型，1 h后再灌注，24 h后治疗组尾静脉注射3×106骨髓基质细胞，盐水对照组尾静脉注射1 mL生理盐水，空白对照组不进行注射。
结果与结论：在梗死后第7，14天治疗组的神经损伤评分明显低于对照组(P < 0.05)；治疗组神经生长因子和脑源性神经营养因子表达在各时间点均高于对照组(P < 0.05)。结果显示静脉移植骨髓基质细胞可改善缺血性脑梗死大鼠神经功能，移植骨髓基质细胞后缺血脑组织中神经生长因子及脑源性神经营养因子表达促进了神经功能的恢复。

关键词: 骨髓基质细胞, 移植, 缺血性脑梗死, 神经生长因子, 脑源性神经营养因子

Abstract:

BACKGROUND: Bone marrow stromal cells (BMSCs) can differentiate into neurons and astrocytes under the appropriate conditions, and secrete soluble molecules to promote neuronal survival.
OBJECTIVE: To observe the effect of test the effect of BMSCs on neurotrophic factors and neurologic function of rats with ischemic cerebral infarction.
METHODS: Rats were subjected to transient middle cerebral artery occlusion and intravenously injected with 3×106 BMSCs (treatment group) and 1 mL normal saline (saline group) at 24 hours after stroke. No administration was done in blank control group.
RESULTS AND CONCLUSION: Significant recovery of neurological function was found in the treatment group at 7 and 14 days after stroke in comparison with the other two groups (P < 0.05). Nerve growth factor and brain derived neurotrophic factor had a higher expression in the treatment group than the other two groups at different time points (P < 0.05). Intravenous injection of BMSCs can improve the neurologic function of rats with ischemic cerebral infarction and the increase in the expression of nerve growth factor and brain derived neurotrophic factor in the ischemic tissue plays an important role in neurological recovery.

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