中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8418-8423.doi: 10.3969/j.issn.1673-8225.2011.45.013

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

当归枸杞制剂诱导脐血单个核细胞分化为肝细胞后人白蛋白的表达

傅京力1，2，曾  妍3，龙则灵1，唐晓鹏1   

  1. 1中南大学湘雅二医院肝病中心，中南大学肝病研究所，湖南省长沙市 410011
    2株洲市一医院感染内科，湖南省株洲市 412000
    3湖南省劳动卫生职业病防治研究所附属医院，湖南省长沙市  410007
  • 收稿日期:2011-04-16 修回日期:2011-05-19 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 唐晓鹏，教授，博士，中南大学湘雅二医院肝病中心, 中南大学肝病研究所，湖南省长沙市 410011 tangxiaopeng2008@163.com
  • 作者简介:傅京力，男，1979年生，湖南省湘潭市人，汉族，2003年中南大学湘雅医学院毕业，主治医师，主要从事传染病研究。 12832333@QQ.com
  • 基金资助:

    国家自然科学基金项目(39870651)；湖南省自然科学基金项目(04JJ6048)。

Angelica sinensis (oliv.) diels and lycium chinense mill preparations induce the differentiation of cord blood mononuclear cells into hepatocyts: Expression of human albumen

Fu Jing-li1,2, Zeng Yan3, Long Ze-ling1, Tang Xiao-peng1   

  1. 1Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha  410011, Hunan Province, China
    2Department of Infection, First Hospital of Zhuzhou City, Zhuzhou  412000, Hunan Province, China
    3Affiliated Hospital, Hunan Institute of Occupational Health, Changsha  410007, Hunan Province, China
  • Received:2011-04-16 Revised:2011-05-19 Online:2011-11-05 Published:2011-11-05
  • Contact: Tang Xiao-peng, Doctor, Professor, Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha 410011, Hunan Province, China tangxiaopeng2008@163.com
  • About author:Fu Jing-li, Attending physician, Second Xiangya Hospital/Institute of Hepatology, Central South University, Changsha 410011, Hunan Province, China; Department of Infection, First Hospital of Zhuzhou City, Zhuzhou 412000, Hunan Province, China 12832333@QQ.com
  • Supported by:

    the National Natural Science Foundation of China, No. 39870651*; the Natural Science Foundation of Hunan Province, No. 04JJ6048*

PDF

300

被引次数

3

摘要：

背景：部分中药能够促进造血干细胞的生长及分化。
目的：观察当归、枸杞制剂促进人脐血单个核细胞生长分化为肝细胞的作用、作用条件及对肝衰竭的治疗效果。
方法：分离人脐血单个核细胞，加入不同剂量的当归及枸杞制剂，比较不同剂量当归及枸杞制剂对脐血单个核细胞及其转化为肝细胞后人白蛋白mRNA及人甲胎蛋白mRNA表达的影响。腹腔内注射D-氨基半乳糖建立大鼠肝衰竭模型，随机分为生理盐水对照组、脐血单个核细胞组、脐血单个核细胞与当归、枸杞制剂灌胃和腹腔内注射免疫抑制剂不同组合8组，检测大鼠肝功能变化。
结果与结论：90 mg/L当归、40 mg/L枸杞制剂可促进单个核细胞的增殖和分化，并加快脐血单个核细胞分化为肝细胞，两者联合无药物协同作用。单独应用当归与枸杞对大鼠肝损伤均有一定的修复作用，与单个核细胞联合均可促进单个核细胞向肝细胞分化，增强修复肝损伤的作用，并且当归的修复效果优于枸杞。加用环磷酰胺后并不能增强当归或枸杞联合单个核细胞的治疗效果。

关键词: 当归, 枸杞, 脐血, 单个核细胞, 细胞分化, 肝细胞

Abstract:

BACKGROUND: Some traditional Chinese medicine can promote the differentiation and growth of human hematopoietic stem cells.
OBJECTIVE: To observe the promotion effect of angelica sinensis (oliv.) diels and lycium chinense mill preparation for the differentiation of human umbilical cord blood stem cells (HUCBSCs) into hepatocytes in vitro and in vivo.
METHODS: HUCBSCs were separated and cultured in different doses of angelica sinensis (oliv.) diels and lycium chinense mill preparation to screen the suitable dosage for HUCBSCs growth. Albumen mRNA and alpha fetoprotein mRNA expression were detected by RT-PCR. The liver failure models of rats were established after D-aminogalactose was injected intraperitoneally. The survival rats with acute hepatic injury were randomly divided into eight groups to detect the changes of liver function.
RESULTS AND CONCLUSION: 90 mg/L angelica sinensis (oliv.) diels and 40 mg/L lycium chinense mill preparation was suitable for HUCBSCs proliferation and differentiation. Both angelica sinensis(oliv.) diels and lycium chinense mill preparation could promote and speed up the differentiation of HUCBSCs into hepatocytes, and the former one was superior to the latter one. However, there was no synergistic effect. The difference between groups treated with/without cyclophosphamide was not obvious.

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