中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7446-7449.doi: 10.3969/j.issn.1673-8225.2011.40.009

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐血间充质干细胞的体外成骨

王  浩1，2，未东兴2   

  1. 1辽宁医学院第五临床医院骨科，辽宁省锦州市121000
    2锦州市中心医院骨科，辽宁省锦州市121000
  • 收稿日期:2011-04-07 修回日期:2011-05-30 出版日期:2011-10-01 发布日期:2011-10-01
  • 作者简介:王浩★，男，1973年生，辽宁省锦州市人，汉族，辽宁医学院在读硕士，副主任医师，主要从事骨损失与修复的研究。 yunshengma@126.com

Osteogenic differentiation of mesenchymal stem cells from human cord blood in vitro

Wang Hao1,2, Wei Dong-xing2   

  1. 1Department of Orthopedic Surgery, the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
    2Department of Orthopedic Surgery, Jinzhou Central Hospital, Jinzhou  121000, Liaoning Province, China
  • Received:2011-04-07 Revised:2011-05-30 Online:2011-10-01 Published:2011-10-01
  • About author:Wang Hao★, Studying for master’s degree, Associate chief physician, Department of Orthopedic Surgery, the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China; Department of Orthopedic Surgery, Jinzhou Central Hospital, Jinzhou 121000, Liaoning Province, China yunshengma@126.com

PDF

347

被引次数

14

摘要：

背景：用免疫细胞阴性筛选法可以排除造血干细胞对于间充质干细胞生长的影响，较快的获得具有表达间充质干细胞的细胞群。
目的：探讨人脐静脉血间充质干细胞向成骨样细胞分化的能力。
方法：用免疫磁珠阳性筛选法分离脐血间充质干细胞后进行培养。取P2代细胞行成骨诱导分化。
结果与结论：人脐血间充质干细胞贴壁生长，长梭形，3周长满瓶底，传代后细胞增殖迅速。细胞表型为高表达CD44+，CD29+和CD166+。在成骨诱导后碱性磷酸酶染色阳性，Von-Kossa染色提示钙化结节形成。提示脐血间充质干细胞能够在体外分化为成骨样细胞。

关键词: 脐血, 间充质干细胞, 诱导分化, 种子细胞, 细胞表型

Abstract:

BACKGROUND: Negative screening for immune cells can remove the effect of hematopoietic stem cells on the growth of mesenchymal stem cells, with rapid access to obtain cell populations to express mesenchymal stem cells.
OBJECTIVE: To study the osteogenic ability of mesenchymal stem cells from human cord blood.
METHODS: The mesenchymal stem cells were isolated by immunomagnetic beads from cord blood. P2 cells were to study the ability of osteogenesis.
RESULTS AND CONCLUSION: The human cord blood mesenchymal stem cells presented spindle shape and reached confluence within 3 weeks. The phenotypes were CD44+, CD29+, CD166+, CD34-. In osteogenic experiment, the cells’ alkaline phosphatase expression was positive, and hydroxyapatite nodules were detected by Von-Kossa staining. The human cord blood mesenchymal stem cells can differentiate into osteoblast-like cells in vitro.

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