中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7031-7035.doi: 10.3969/j.issn.1673-8225.2011.38.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

转染血管内皮细胞生长因子骨髓间充质干细胞与牛松质骨支架复合组织工程骨的血管形成

陈  毅1，尹西盟1，韩亚新1，王岩峰1，刘丹平2，屠冠军1   

  1. 1中国医科大学附属第一医院骨科，辽宁省沈阳市  110001
    2辽宁医学院附属第一医院骨科，辽宁省锦州市 121001
  • 收稿日期:2011-02-06 修回日期:2011-03-06 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 屠冠军，教授，中国医科大学附属第一医院骨科，辽宁省沈阳市 110001 tgj188@gmail.com
  • 作者简介:陈毅☆，男，1982年生，浙江省金华市人，汉族，中国医科大学在读博士，医师，主要从事组织工程骨血管再生研究。 chenyi0427163@163.com
  • 基金资助:

    国家自然科学基金项目(81070971)。

Vascular endothelial growth factor transfected bone marrow mesenchymal stem cells cultured on bovine cancellous bone scaffold promote the formation of new vessels

Chen Yi1, Yin Xi-meng1, Han Ya-xin1, Wang Yan-feng1, Liu Dan-ping2, Tu Guan-jun1   

  1. 1Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang  110001, Liaoning Province, China
    2Department of Orthopaedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Received:2011-02-06 Revised:2011-03-06 Online:2011-09-17 Published:2011-09-17
  • Contact: Tu Guan-jun, Professor, Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China tgj188@gmail.com
  • About author:Chen Yi☆, Studying for doctorate, Physician, Department of Orthopaedics, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China chenyi0427163@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81070971*

PDF

249

被引次数

5

摘要：

背景：小块组织工程骨植入动物体内后，早期依靠组织液的渗透可获得营养，但大块组织工程骨的营养仅靠组织液的渗透是远远不够的，必须通过血管再生来获得。
目的：观察转染血管内皮细胞生长因子表达载体骨髓间充质干细胞复合组织工程骨植入动物体内后的血管形成能力。
方法：制作日本大耳白兔双侧尺骨中段骨缺损模型，左侧尺骨缺损植入转染血管内皮细胞生长因子表达载体的自体骨髓间充质干细胞复合脱钙脱脂去蛋白的牛松质骨支架组织工程骨为实验组，右侧尺骨缺损植入自体骨髓间充质干细胞复合脱钙脱脂去蛋白的牛松质骨支架组织工程骨为对照组。术后12周行X射线摄片观察、大体标本观察、苏木精-伊红染色和Masson染色组织切片观察、MiFas图像分析系统定量分析。
结果与结论：实验组与对照组尺骨缺损处均有连续骨痂形成；组织切片经苏木精-伊红染色、Masson三色法染色及MiFas图像分析系统定量分析可见实验组和对照组均有大量的新生骨，但实验组新生血管明显多于对照组(P < 0.01)，且血管较粗大，而且与新生骨接近。说明将转染血管内皮细胞生长因子表达载体的骨髓间充质干细胞复合在组织工程骨上植入动物体内可明显促进新生血管的形成。

关键词: 脱钙脱脂去蛋白的牛松质骨支架, 骨髓间充质干细胞, 血管内皮细胞生长因子, 组织工程骨, 血管生成

Abstract:

BACKGROUND: After a small tissue engineering bone transplanted into animals, which can obtain enough nutrition by interstitial fluid penetration, but if a big one, it is insufficient by interstitial fluid penetration and must obtained by revascularization.
OBJECTIVE: To observe the ability of bone marrow mesenchymal stem cells (BMSCs) transfected with vascular endothelial growth factor (VEGF) expression vector to promote vascularization on tissue engineering bone.
METHODS: Bone defect model of the middle piece of both hand side ulnar bones of Japanese big ear rabbits was made. Transfected VEGF expression vector of autologous BMSCs to the protein of defatted bovine demineralized cancellous bone tissue engineering bone scaffold was implanted into left ulna defect as the experimental group. Autologous BMSCs combined with decalcifying, defatted and protein-free bovine cancellous bone scaffold of tissue engineering bone as the control group. At 12 weeks after operation, X ray photo observation, general specimen observation, hematoxylin-eosin staining and Masson staining histological section observation, MiFas picture analysis system quantitative analysis were performed.
RESULTS AND CONCLUSION: The continuous calluses were formed in ulnar bone defect between experimental and control groups. Tissue section was stained with hematoxylin-eosin staining and Masson staining, and observed by MiFas picture analysis system quantitative analysis, which showed a large number of new bones between experimental and control groups, but new vessels in experimental group was significantly more than that in control group (P < 0.01); vessles were thick and closed to new bones. It is indicated that BMSCs of transfected VEGF expression vector combined with tissue engineering bone and implanted into animals can obviously promote the formation of new vessels.

中图分类号: