中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6967-6971.doi: 10.3969/j.issn.1673-8225.2011.37.030

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

痹肿消汤干预胶原诱导关节炎模型大鼠关节滑膜组织细胞外信号调节激酶1/2及细胞外信号调节激酶5的表达

徐则林1，梁清华1，陈  疆2，熊新贵1，郭亚静1，肖玉美1   

  1. 中南大学湘雅医院，1中西医结合研究所，2远程医疗中心，湖南省长沙市  410008
  • 收稿日期:2011-02-15 修回日期:2011-02-21 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 梁清华，教授，博士生导师。中南大学湘雅医院中西医结合研究所，湖南省长沙市 410008 lqhxy@126.com 通讯作者：陈疆，硕士，工程师，中南大学湘雅医院远程医疗中心，湖南省长沙市 410008 cjxyz@126.com
  • 作者简介:徐则林★，女，1986年生，湖南省长沙市人，中南大学在读硕士，主要研究方向为风湿免疫与脑血管病。 751825767@qq.com
  • 基金资助:

    国家教育部高校博士点基金(200805330011)，湖南省科技计划项目(2009SK3163)，湖南省中医药基金(2010038)。

Effects of Bizhongxiao decoction on extracellular signal-regulated kinase 1/2 and 5 expression in the synovial tissue in a rat model of collagen-induced arthritis

Xu Ze-lin1, Liang Qing-hua1, Chen Jiang2, Xiong Xin-gui1, Guo Ya-jing1, Xiao Yu-mei1   

  1. 1Institute of Combined Traditional Chinese and Western Medicine, 2Remote Medical Center, Xiangya Hospital of Central South University, Changsha   410008, Hunan Province, China
  • Received:2011-02-15 Revised:2011-02-21 Online:2011-09-10 Published:2011-09-10
  • Contact: Liang Qing-hua, Professor, Doctoral supervisor, Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China lqhxy@126.com Correspondence to: Chen Jiang, Master, Engineer, Remote Medical Center, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China cjxyz@126.com
  • About author:Xu Ze-lin★, Studying for master’s degree, Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China 751825767@qq.com
  • Supported by:

    Ph.D. Programs Foundation of Ministry of Education of China, No. 200805330011*; Science and Technology Plan Program of Hunan Province, No. 2009SK3163*; Traditional Chinese Medicine Foundation of Hunan Province, No. 2010038*

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289

被引次数

2

摘要：

背景：滑膜细胞信号转导异常是类风湿性关节炎的重要发病机制之一。中药复方痹肿消汤治疗类风湿性关节炎具有较好的临床疗效，但在细胞信号转导水平探讨痹肿消汤治疗类风湿性关节炎的作用机制是十分必要的。
目的：观察中药复方痹肿消汤对胶原诱导关节炎模型大鼠滑膜细胞外信号调节激酶1/2，5表达的影响。
方法：SD大鼠45只随机数字表法分为对照组、模型组、痹肿消汤组。胶原诱导关节炎模型大鼠，用痹肿消汤药液3.0~3.5 mL灌胃，1次/d，连续14 d。检测造模后第14，21，28天大鼠滑膜细胞外信号调节激酶1/2，5的磷酸化水平。
结果与结论：造模后，大鼠滑膜细胞外信号调节激酶1/2，5的磷酸化表达随时间而增加(P < 0.05)。痹肿消汤治疗后，细胞外信号调节激酶1/2，5磷酸化表达水平则明显下降(P < 0.05)。提示痹肿消汤能抑制细胞外信号调节激酶1/2，5蛋白激酶的激活，从而抑制细胞外信号调节激酶1/2， 5信号转导通路的活化，这可能是其阻抑类风湿性关节炎滑膜异常增殖、关节骨质侵蚀的重要机制之一。

关键词: 痹肿消汤, 胶原诱导性关节炎, 细胞外信号调节激酶1/2, 细胞外信号调节激酶5, 免疫印迹法, 滑膜

Abstract:

BACKGROUND: Abnormal signal transduction of synovial cells is one of important pathogeneses of rheumatoid arthritis (RA). Chinese herbs Bizhongxiao decoction exhibits satisfactory clinical curative effects in treatment of RA. It is necessary to investigate the mechanism by which Bizhongxiao decoction treats RA at the level of cellular signal transduction.
OBJECTIVE: To investigate the effects of Bizhongxiao decoction on extracellular signal-regulated kinase (ERK) 1/2 and ERK 5 expression in the synovial tissue in a rat model of collagen-induced arthritis.
METHODS: Forty-five SD rats were randomly divided into three groups: control, model and Bizhongxiao deconction. Rat models of collagen-induced arthritis were intragastrically administered with 3.0-3.5 mL Bizhongxiao decoction, once a day, for successive 14 days. At 14, 21 and 28 days after arthritis induction, ERK 1/2 and ERK 5 expression was detected.
RESULTS AND CONCLUSION: After arthritis induction, ERK 1/2 and ERK 5 expression in the synovial tissue was increased with elongation of time (P < 0.05). After Bizhongxiao decoction treatment, ERK 1/2 and ERK 5 expression levels were significantly decdreased (P < 0.05). Results showed that Bizhongxiao decoction can inhibit the activation of ERK1/2, and ERK 5 and thereby inhibit the activation of ERK 1/2 and ERK 5 signal transduction pathway. This may be one of important mechanisms by which Bizhongxiao decoction inhibits abnormal proliferation of synovial tissue and the erosion of articular bone in RA.

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