中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6871-6874.doi: 10.3969/j.issn.1673-8225.2011.37.008

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

转化生长因子β1在兔房水中的浓度变化

吴  伟1，廖国平2，陆晓和1，梁丽芳1，闫丽梦1，陈  晗1，张海峰1，徐  微1，魏二霞1   

  1. 南方医科大学珠江医院，1 眼科，2 药剂科，广东省广州市  510282
  • 收稿日期:2011-04-23 修回日期:2011-08-01 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 陆晓和，博士，博士生导师，主任医师，教授，南方医科大学珠江医院眼科，广东省广州市 510282 luophthal@126. com
  • 作者简介:吴伟★，男，1985年生，河南省信阳市人，汉族，南方医科大学在读硕士，主要从事眼表疾病的基础与临床研究。 Wuwei200414952@126.com

Concentrations of transforming growth factor beta 1 in rabbit aqueous humor

Wu Wei1, Liao Guo-ping2, Lu Xiao-he1, Liang Li-fang1, Yan Li-meng1, Chen Han 1, Zhang Hai-feng1, Xu Wei1, Wei Er-xia1   

  1. 1Department of Ophthalmology, 2Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-04-23 Revised:2011-08-01 Online:2011-09-10 Published:2011-09-10
  • Contact: Lu Xiao-he, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 515282, Guangdong Province, China luophthal@126.com
  • About author:Wu Wei★, Studying for master’s degree, Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Wuwei200414952@ 126.com

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摘要：

背景：转化生长因子β1可参与角膜损伤后的修复。
目的：观察转化生长因子β1滴眼液滴眼后房水中的浓度变化规律。
方法：将新西兰大白兔随机分为5组，分别给予PBS和质量浓度为0.5，1.0，2.0，4.0 mg/L的转化生长因子β1滴眼液滴右眼。
结果与结论：通过裂隙灯观察兔角膜和结膜结构，各组兔眼均无结膜分泌物、球结膜充血、角膜水肿增厚、角膜后沉着物、前房炎性反应及晶状体混浊改变。ELISA检测结果显示，与PBS组比较，质量浓度2.0和4.0 mg/L转化生长因子β1滴眼液能有效提高兔眼房水中转化生长因子β1的质量浓度(P < 0.01)，角膜穿透性良好，在房水中可以达到有效的治疗浓度。

关键词: 转化生长因子&beta, 1, 房水, 角膜, 兔, 组织构建

Abstract:

BACKGROUND: Studies has demonstrated that transforming growth factor β1(TGF-β1) eyedrops is not only involved in the tissue metabolism of normal eye as well as kinds of physiological function, but also plays an important role in the treatment effect on corneal wound healing, uveitis and nearsightedness .
OBJECTIVE: To investigate the concentration of TGF-β1 in aqueous humor after ophthalmic administration and to establish the method for quantitatively analyzing the TGF-β1 levels in rabbit aqueous humor.
METHODS: Thirty rabbits were randomly divided into blank control, TGF-β1 0.5, 1.0, 2.0, 4.0 mg/L groups, with six animals in each group. PBS and 0.5, 1.0, 2.0,4.0 mg/L TGF-β1 were dropped into the right eyeballs four times per day for 1 week. 
RESULTS AND CONCLUSION: No conjunctival secretion, bulbar conjunctiva hyperemia, corneal edema, keratic precipitates, inflammation in anterior chamber, or vitreous haze lens opacity was found prior to and following experiments in each group. ELISA results showed that compared with PBS, both 2.0 mg/L and 4.0 mg/L TGF-β1 eyedrops can significantly improve the TGF-β1 concentration effectively in rabbit aqueous humor (P < 0.05), which has a good penetrating ability in the cornea, and can effectively enhance treatment concentration in aqueous humor.

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