中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6705-6708.doi: 10.3969/j.issn.1673-8225.2011.36.013

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

5-氮杂胞苷诱导人脐带间充质干细胞向心肌样细胞的分化

阮中宝1，杨向军1，陈各才2，朱  莉2，李  伟3，杨  兵3，欧阳溪3，潘少辉3   

  1. 1苏州大学附属第一医院心内科，江苏省苏州市  215006
    2泰州市人民医院心内科，江苏省泰州市 225300
    3江苏省北科生物科技有限公司，江苏省泰州市  225300
  • 收稿日期:2011-06-28 修回日期:2011-07-13 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 杨向军，主任医师，教授，博士生导师，苏州大学附属第一医院心内科，江苏省苏州市215006 yxjdemail@163.net
  • 作者简介:阮中宝☆，男，1970年生，江苏省兴化市人，汉族，苏州大学在读博士，副主任医师，主要从事心电生理介入治疗及干细胞移植治疗方面的研究。 ruanzhongbao@medmail.com.cn

5-azacytidine induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes

Ruan Zhong-bao1, Yang Xiang-jun1, Chen Ge-cai2, Zhu Li2, Li Wei3, Yang Bing3, Ouyang Xi3, Pan Shao-hui3   

  1. 1Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China
    2Department of Cardiology, Taizhou People’s Hospital, Taizhou  225300, Jiangsu Province, China
    3Jiangsu BeiKe Bio-Technology, Taizhou 225300, Jiangsu Province, China
  • Received:2011-06-28 Revised:2011-07-13 Online:2011-09-03 Published:2011-09-03
  • Contact: Yang Xiang-jun, Chief physician, Professor, Doctoral supervisor, Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China yxjdemail@163.net
  • About author:Ruan Zhong-bao☆, Studying for doctorate, Associate chief physician, Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China ruanzhongbao@medmail.com.cn

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374

被引次数

15

摘要：

背景：5-氮杂胞苷能诱导人脐带间充质干细胞分化为心肌细胞。
目的：以5-氮杂胞苷诱导人脐带间充质干细胞分化为心肌细胞。
方法：采用贴壁培养法分离、纯化人脐带间充质干细胞，以5-氮杂胞苷诱导第3代人脐带间充质干细胞分化为心肌样细胞。
结果与结论：诱导前人脐带间充质干细胞呈典型的梭形；诱导后一二周细胞体积变大，三四周后相邻细胞间胞膜有接触，逐渐相连呈肌管状，细胞胞质内可见细丝样结构。诱导4周后免疫组织化学鉴定人脐带间充质干细胞cTnI表达阳性，未诱导细胞cTnI表达阴性；诱导组Nkx2.5、GATA 4 mRNA表达水平较未诱导组显著增加(P < 0.05)。提示5-氮杂胞苷可能通过调控GATA4、Nkx2.5基因的表达促进人脐带间充质干细胞分化为心肌样细胞，并促进其成熟。

关键词: 脐带, 间充质干细胞, 细胞分化, 心肌细胞, 5-氮杂胞苷

Abstract:

BACKGROUND: 5-azacytidine (5-aza) can induce the differentiation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) into cardiomyocytes.
OBJECTIVE: To investigate the cellular mechanism underlying the differentiation of hUCMSCs into cardiomyocytes induces by 5-aza.
METHODS: hUCMSCs were isolated and purified by adherent method. hUCMSCs at passage 3 were induced to differentiate into cardiomyocytes by 5-aza.
RESULTS AND CONCLUSION: Spindle-shaped hUCMSCs were presented from human umbilical cord tissue by adherent culture method. hUCMSCs treated with 5-aza had long cytoplasmic process 1-2 weeks after induction, and they were connected with adjacent cells forming myotube-like structures 3-4 weeks after induction. hUCMSCs treated with 5-aza were stained positively for cTnI 4 weeks after induction. Statistically higher level expression of Nkx2.5 and GATA 4 mRNA in induced group was observed in comparison with those of control group (P < 0.05). These indicated that hUCMSCs can be induced to differentiate into cardiomyocytes by 5-aza in vitro through up-regulating the expression of Nkx2.5 and GATA4.

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