中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6687-6690.doi: 10.3969/j.issn.1673-8225.2011.36.009

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

贴壁和缩短胰酶消化时间培养大鼠骨髓间充质干细胞

李树法1，张  敏2   

  1. 贵阳市第一人民医院，1 内分泌科，2 中华社区卫生服务中心，贵州省贵阳市550002
  • 收稿日期:2011-01-13 修回日期:2011-04-10 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 张敏，主治医师，贵阳市第一人民医院中华社区卫生服务中心，贵州省贵阳市 550002 864769653@qq.com
  • 作者简介:李树法☆，男，1974年生，山东省临沂市人，汉族，2011年解放军第三军医大学毕业，博士，副主任医师，主要从事从事内分泌代谢性疾病研究。 shufali@163.com

Culture of rat bone marrow mesenchymal stem cells by using adherent method and shortening the time of trypsin digestion

Li Shu-fa1, Zhang Min2   

  1. 1Department of Endocrinology, 2Zhonghua Community Health Center, the First People’s Hospital of Guiyang, Guiyang  550002, Guizhou Province, China
  • Received:2011-01-13 Revised:2011-04-10 Online:2011-09-03 Published:2011-09-03
  • Contact: Zhang Min, Attending physician, Zhonghua Community Health Center, the First People’s Hospital of Guiyang, Guiyang 550002, Guizhou Province, China 864769653@qq.com
  • About author:Li Shu-fa☆, Doctor, Associate chief physician, Department of Endocrinology, the First People’s Hospital of Guiyang, Guiyang 550002, Guizhou Province, China shufali@163.com

PDF

428

被引次数

6

摘要：

背景：对于骨髓间充质干细胞的分离纯化、培养扩增目前已有几种方法，各自均有不同的优劣性。
目的：观察贴壁法和缩短胰酶消化时间的方法体外分离和培养大鼠骨髓间充质干细胞的特点。
方法：培养初期频繁换液，去除混杂细胞；传代后加入不同诱导剂定向诱导分化，使之分别向脂肪细胞，成骨细胞和软骨细胞方向分化。
结果与结论：该分离培养方法在第3代可以得到纯化的骨髓间充质干细胞。分离培养的细胞纯度高，有良好的增殖和分化潜能。定向诱导培养的细胞能向成脂细胞、成骨细胞和成软骨细胞方向分化。

关键词: 骨髓间充质干细胞, 大鼠, 分离培养, 细胞分化, 鉴定

Abstract:

BACKGROUND: There is no uniform standard for bone marrow mesenchymal stem cells (BMSCs) isolation, purification, culture and expansion, and no specific marker for BMSCs identification.
OBJECTIVE: To investigate the different isolation and culture methods of BMSCs.
METHODS: Rat BMSCs were isolated and cultured by the method of frequent replacement of culture medium at early stage, controlling trypsin digestion time and plastic adherent culture. The cultured cells were induced under different conditions. The differentiated adipoccytes, osteoblasts and chondrocytes were observed.
RESULTS AND CONCLUSION: Purified BMSCs can be obtained at the third generation with this isolation and culture method. The cultured cells have good proliferative potential. BMSCs can differentiate to adipocytes, osteoblasts and chondrocytes under certain conditions.

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