中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6375-6378.doi: 10.3969/j.issn.1673-8225.2011.34.025

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

基于改性壳聚糖纳米胶束的制备和表征及其细胞毒性评价

蒋刚彪1，林钻涛1，徐晓鹏2，黄志坚2，戚何妹2   

  1. 1华南农业大学资环学院制药工程系，广东省广州市   510642
    2中山大学生命科学院，广东省广州市  510275
  • 收稿日期:2011-05-25 修回日期:2011-07-18 出版日期:2011-08-20 发布日期:2011-08-20
  • 作者简介:蒋刚彪☆，男，湖南省邵阳市人，汉族，2005年中山大学毕业，博士，副教授，主要从事天然生物材料方面的研究。 gbjiang@scau.edu.cn jgb3h@163.com
  • 基金资助:

    国家自然基金面上项目(20874032)；广东省科技计划项目(2008B030301202)。广东省教育部产学研结合项目(2009B090300231)。

Preparation, characterization and cytotoxicity evaluation of polymeric nanomicelles based on modified chitosan

Jiang Gang-biao1, Lin Zhuan-tao1, Xu Xiao-peng2, Huang Zhi-jian2, Qi He-mei2   

  1. 1Department of Pharmaceutical Engineering, College of Natural Resource and Environment, South China Agricultural University, Guangzhou  510642, Guangdong Province, China
    2School of Life Sciences, Sun Yat-sen University, Guangzhou  510275, Guangdong Province, China
  • Received:2011-05-25 Revised:2011-07-18 Online:2011-08-20 Published:2011-08-20
  • About author:Jiang Gang-biao☆, Ph.D., Associate professor, Department of Pharmaceutical Engineering, College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, Guangdong Province, China gbjiang@scau.edu.cn jgb3h@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 20874032*; the Science and Technology Plan of Guangdong Province, No. 2008B030301202*; the Combination Program of Guangdong Province, Ministry of Education, No. 2009B090300231*

PDF

269

被引次数

7

摘要：

背景：壳聚糖进行化学改性以制备性能优良且细胞毒性低、生物相容性高、可降解的聚合物纳米胶束，在药物控释、组织工程等领域获得普遍应用。
目的：将强疏水链十八烷氧基接枝到N-琥珀酰壳聚糖分子中，制备结构稳定、粒径较小的聚合物胶束，以获得可适用于药物传递载体或医学生物材料领域的聚合物纳米胶束。
方法：用N-琥珀酰壳聚糖和十八烷基缩水甘油醚反应，在壳聚糖衍生物中引入长链疏水基。
结果与讨论：成功制备出聚合物纳米胶束，通过红外光谱和1H核磁波谱表征，证实壳聚糖分子中成功引入了琥珀酰基和十八烷氧基。通过动态激光散射测量出其粒径在136~166 nm，其临界胶束溶度在1.67×10-3~3.35×10-3 g/L，反映出胶束具有非常好的稳定性，而且其临界胶束浓度值和粒径随着疏水链接枝率的增加而减少。MTT法检测说明该胶束对细胞毒性极低。

关键词: 聚合物纳米胶束, 两亲性壳聚糖衍生物, 琥珀酰壳聚糖, 胶束, 细胞毒性

Abstract:

BACKGROUND: Chitosan, a natural polymer, can be chemically modified to prepare excellent biocompatible, biodegradable and low-cytotocitable polymeric nanomicelles, which has potent potential in the field of drug controlled release and tissue engineer.
OBJECTIVE: To graft a strong hydrophobic long chain, octadecyl alkoxy, onto succinyl chitosan to synthesize a novel kind of polymeric micelle with good stability and minute size, which can be utilized as drug carries or biomedical materials.
METHODS: Amphiphilic chitosan derivative MCS was obtained by introducing long hydrophobic chain to modify hydrophilic chitosan, which can form stable nanomicelles in aqueous solution. Octadecyl alkyl glycidyl ether was employed as hydrophobic group provider to react with N-succinyl chitosan.
RESULTS AND CONCLUSION: FTIR and 1H-NMR proved that the amphiphilic chitosan derivative (MCS) was synthesized successfully. The polymeric nanomicelles forming by MCS in water were 136-166 nm with negative charged surface. The values of critical micellar solubility of MCS micelles were in the ring from 1.67×10-3 to 3.35×10-3 g/L. The critical micellar solubility and size of the nannomicelles significantly increased with the increase of the substitute degree of hydrophobic group. The results of MTT assay illustrated that MCS can be a safe vehicle for drug.

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