中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6216-6219.doi: 10.3969/j.issn.1673-8225.2011.33.033

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

胶原诱导性关节炎大鼠滑膜组织中磷酸化p38与JNK通路及痹肿消汤的影响

肖玉美，熊新贵，陈  疆，梁清华，郭亚静，徐则林，吴  丹，范  荣   

  1. 中南大学湘雅医院中西医结合科，湖南省长沙市 410008
  • 收稿日期:2011-03-05 修回日期:2011-06-11 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 梁清华，教授，博士生导师，中南大学湘雅医院中西医结合科，湖南省长沙市 410008 Lqhxy@126.com
  • 作者简介:肖玉美★，女，1983年生，湖南省邵阳市人，汉族，中南大学在读硕士，主要从事中西医结合脑血管及风湿免疫临床与实验研究。 179423557@qq.com
  • 基金资助:

    课题受国家教育部高校博士点基金(200805330011)，湖南省科技计划项目(2009SK3163)和湖南中医药基金(2010038)资助。

Effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in synovium of rats with collagen-induced arthritis

Xiao Yu-mei, Xiong Xin-gui, Chen Jiang, Liang Qing-hua, Guo Ya-jing, Xu Ze-lin, Wu Dan, Fan Rong   

  1. Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Received:2011-03-05 Revised:2011-06-11 Online:2011-08-13 Published:2011-08-13
  • Contact: Liang Qing-hua, Professor, Doctoral supervisor, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China Lqhxy@126.com
  • About author:Xiao yu-mei,★ Studying for master’s degree, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China 179423557@qq.com
  • Supported by:

    Ph.D. Program Foundation of Ministry of Education of China, No.200805330011*; Science and Technology Planning Program of Hunan Province, No.2009SK3163*; Traditional Chinese Medicine Foundation of Hunan Province, No. 2010038*

PDF

299

被引次数

5

摘要：

背景：有研究报道滑膜细胞信号传导异常是类风湿关节炎发病的重要机制之一。 
目的：观察胶原诱导性关节炎大鼠中丝裂原活化蛋白激酶通路中磷酸化p38和磷酸化JNK的活化及痹肿消汤的影响。
方法：将72只SD大鼠随机分为正常组、模型组、痹肿消汤组。模型组及痹肿消汤组大鼠足趾注射胶原蛋白乳剂制备胶原诱导性关节炎模型大鼠，10 d后加强免疫，初次免疫后第14天开始给痹肿消汤组大鼠每天痹肿消汤灌胃，模型组蒸馏水灌胃，正常组自由饮水。
结果与结论：Western blot 检测结果显示，胶原诱导性关节炎大鼠中磷酸化p38及JNK的表达较正常组显著增高(P < 0.05)。与模型组相比，痹肿消汤组能下调磷酸化p38及JNK蛋白的表达(P < 0.05)。说明痹肿消汤可抑制类风湿关节炎中磷酸化p38及JNK的高表达。

关键词: 痹肿消汤, 胶原诱导性关节炎, 滑膜, 丝裂原活化蛋白激酶, p38, JNK, 组织构建

Abstract:

BACKGROUND: Reports have shown that abnormal synovial cell signaling is one of important mechanisms of rheumatoid arthritis.
OBJECTIVE: To investigate the effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in mitogen-activated protein kinase (MAPK) pathway in synovium of rats with collagen-induced arthritis.
METHODS: A total of 72 Sprague-Dawley rats were randomly and evenly divided into three groups: normal, model, and Bizhongxiao decoction. In the model and Bizhongxiao decoction groups, rat models of collagen-induced arthritis were created by injection of typeⅡcollagen and complete Freud’s adjuvant into rat toes. After 10 days, rats were immunized again. From day 14 after primary immunization, the Bixiaozhong decoction group rats were intragastrically administered Bizhongxiao decoction every day, the model group rats were identically given distilled water, and the normal group rats were allowed to drink water freely.
RESULTS AND CONCLUSION: Western blot analysis results showed that phosphorylated p38 and JNK expressions were significantly greater in the collagen-induced arthritis rats than in the normal controls (P < 0.05). Compared with the model group, phosphorylated p38 and JNK expressions were significantly downregulated in the Bizhongxiao decoction group (P < 0.05). These findings suggest that Bizhongxiao decoction can inhibit phosphorylated p38 and JNK expressions in rheumatoid arthritis.

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