中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6085-6089.doi: 10.3969/j.issn.1673-8225.2011.33.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

氟影响成骨细胞转化生长因子超家族成员表达的传导通路

赵  阳1，何铁英2，张亚楼3   

  1. 新疆医科大学第一附属医院，1烧伤整形科，2胰腺外科，新疆维吾尔自治区乌鲁木齐市  830054；3新疆医科大学基础医学院组织胚胎学教研室，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2011-03-05 修回日期:2011-06-21 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 张亚楼，博士，助理研究员，新疆医科大学基础医学院组织胚胎学教研室，新疆维吾尔自治区乌鲁木齐市 830054 zhangyalou12@yahoo.com.cn
  • 作者简介:赵阳☆，男，1974年生，新疆维吾尔自治区哈密市人，南方医科大学在读博士，主要从事疾病诊断的基础研究。 gorgezy@163. com 并列第一作者：何铁英，女，1977年生，新疆维吾尔自治区乌鲁木齐人，新疆医科大学在读博士，主要从事疾病的分子诊断研究。 tietie5309@163.com
  • 基金资助:

    国家自然科学基金(30860249)。

Fluoride influences transduction pathway of transforming growth factor superfamily members expressed in osteoblasts

Zhao Yang1, He Tie-ying2, Zhang Ya-lou3   

  1. 1Department of Burn and Plastic Surgery, 2Department of Pancreatic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 3Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-03-05 Revised:2011-06-21 Online:2011-08-13 Published:2011-08-13
  • Contact: Zhang Ya-lou, Doctor, Assistant Researcher, Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zhangyalou12@ yahoo.com.cn
  • About author:Zhao Yang☆, Studying for doctorate, Department of Burn and Plastic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China gorgezy@163.com He Tie-ying, Studying for doctorate, Department of Pancreatic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China tietie5309@163.com Zhao Yang and He Tie-ying contributed equally to this paper and were considered as co-first authors.
  • Supported by:

    the National Natural Science Foundation of China, No. 30860249*

PDF

383

被引次数

7

摘要：

背景：氟中毒骨转换过程中转化生长因子β超家族成员参与了骨转换过程，但是机制不清楚。
目的：观察氟对体外培养成骨细胞中转化生长因子β1，骨形态发生蛋白2和SMAD4信号转导通路的影响。
方法：体外培养人成骨细胞，按染氟(NaF)剂量将成骨细胞分为0(对照)，0.625，1.25，2.5，5，10，20，40，80，160 mg/L组进行实验观察。
结果与结论：氟对成骨细胞增殖影响与转化生长因子β1，骨形态发生蛋白2表达有密切的关系。当氟质量浓度为0．625 mg/L时，氟化物产生的效应主要通过转化生长因子β1，骨形态发生蛋白2，SMAD4信号转导通路调节。当低于或超过此剂量时，氟化物产生的效应与信号转导通路关系不大，可能存在转化生长因子β1及骨形态发生蛋白2其他传导通路的调节。

关键词: 氟化物中毒, 人成骨细胞, 转化生长因子&beta, 1, 骨形态发生蛋白2, SMAD4, 信号转导通路

Abstract:

BACKGROUND: Transforming growth factor (TGF) beta superfamily members are involved in bone turnover process in fluorosis, but the mechanism remains poorly understood.
OBJECTIVE: To investigate the effects of fluoride on TGF-β1, BPM-2 and SMAD4 signal transduction pathway of osteoblasts cultured in vitro.
METHODS: Human osteoblasts were cultured in vitro and then grouped according to (NaF) dose used: 0 (control), 0.625, 1.250, 2.500, 5.000, 10.000, 20.000, 40.000, 80.000, 160.000 mg/L．Cells were collected after 24 hours of culture, RNA extracted, and the mRNA expression of TGF-β1, BPM-2 and SMAD4 was detected using fluorescent quantitative reverse transcription polymerase chain reaction.
RESULTS AND CONCLUSION: Fluoride may affect mRNA expression of TGF-β1, BPM-2 in osteoblasts and their expression level was related to fluoride concentration. When NaF was 0.625 mg/L, NaF effect on osteoblasts was regulated via TGF-β1, BPM-2 and SMAD4 signal transduction pathway. When NaF was higher or lower than 0.625 mg/L, NaF effect on osteoblasts was not related to TGF-β1, BPM-2 and SMAD4 signal transduction pathway. There may be other transduction pathways involved in this process.

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