中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5928-5931.doi: 10.3969/j.issn.1673-8225.2011.32.009

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

引产儿与正常成人骨髓间充质干细胞的比较

程月爱，刘瑞风，张永翠，张开明   

  1. 太原市中心医院皮肤科，山西省太原市  030009
  • 收稿日期:2011-02-28 修回日期:2011-04-26 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 张开明，博士，主任医师，太原市中心医院皮肤科，山西省太原市 030009 zhangkaiming@sina.com
  • 作者简介:程月爱，女，1967年生，山西省朔州市人，汉族，1991年山西职工医学院毕业，主管护师，主要从事皮肤科激光美容及银屑病发病机制的研究。 1053881306@qq.com
  • 基金资助:

    国家自然科学基金资助项目(30972657)，课题名称：银屑病患者骨髓造血干细胞定向分化为 T 细胞过程Notch 信号转导通路的动态研究。

Comparison of bone marrow-derived mesenchymal stem cells from aborted fetus and normal adult subjects

Cheng Yue-ai, Liu Rui-feng, Zhang Yong-cui, Zhang Kai-ming   

  1. Department of Dermatology, Taiyuan Central Hospital, Taiyuan  030009, Shanxi Province, China
  • Received:2011-02-28 Revised:2011-04-26 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhang Kai-ming, Doctor, Chief physician, Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, Shanxi Province, China zhangkaiming@sina.com
  • About author:Cheng Yue-ai, Nurse in charge, Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, Shanxi Province, China 1053881306@qq.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30972657*

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摘要：

背景：骨髓间充质干细胞是多能干细胞，同时可参与免疫调节反应，而成人及引产胎儿骨髓均是间充质干细胞的重要来源。
目的：通过引产儿与正常成人骨髓间充质干细胞的细胞形态、免疫表型、增殖活性及分泌细胞因子的比较，揭示二者生物学特点的差异。
方法：采用密度梯度离心法分离引产儿与正常成人骨髓单个核细胞，通过贴壁法培养骨髓间充质干细胞，收集传3代后的骨髓间充质干细胞，流式细胞术鉴定其免疫表型、MTT比色法检测其增殖活性以及用ELISA法检测细胞培养上清液中白细胞介素6、血小板源性生长因子和表皮生长因子的水平。
结果与结论：引产儿组和正常成人组骨髓间充质干细胞形态相似、表型相同，分泌细胞因子水平无显著差异，但引产儿细胞增殖活性显著高于正常成人。提示引产儿与正常成人骨髓间充质干细胞除增殖活性有差异外，其余生物学特点相似，引产儿可以为间充质干细胞研究提供新的骨髓来源。

关键词: 引产儿, 成人, 骨髓, 间充质干细胞, 生物学特点

Abstract:

BACKGROUND: Bone marrow-derived mesenchymal stem cells are pluripotent cells and can be involved in immune response. Fetal and adult bone marrow is both important sources for mesenchymal stem cells.
OBJECTIVE: To reveal the differences of bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects by comparing the biological characters (including morphology, immunophenotype, proliferative activity and cytokines secretion).
METHODS: Bone marrow mononuclear cells of aborted fetus and normal adult subjects were separated by density gradient centrifugation. Bone marrow derived mesenchymal stem cells were cultured with the adherent method. Cells at passage 3 and their culture supernatants were collected. Flow cytometry analysis was used to examine the immunophenotype. The proliferation activity of cells was measured by MTT colorimetric assay. ELISA was used to measure the concentrations of cytokines secreted from bone marrow derived mesenchymal stem cells in the two groups. 
RESULTS AND CONCLUSION: Cells from both groups have the same morphology, immunophenotype and the levels of cytokines secretion. But in comparison with normal adult subjects, bone marrow derived mesenchymal stem cells from aborted fetus showed higher proliferative activity.  Bone marrow derived mesenchymal stem cells from aborted fetus and normal adult subjects have the similar biological characteristics except the proliferative activity. Aborted fetus can provide bone marrow for research of stem cells.

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