中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5901-5904.doi: 10.3969/j.issn.1673-8225.2011.32.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

胚胎骨髓来源间充质干细胞对人Th17细胞的免疫调节

郭振兴1，郑翠玲2，陈振萍3，董文川1，杨仁池4   

  1. 1清华大学第一附属医院血液肿瘤科，北京市100016
    2中国医学科学院肿瘤医院检验科，北京市 100029
    3北京儿童医院血液中心，北京市 100045
    4中国医学科学院血液学研究所实验血液学国家重点实验室，天津市 300020
  • 收稿日期:2011-02-28 修回日期:2011-03-28 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:郭振兴☆，男，2008年中国协和医科大学毕业，博士，主要从事干细胞免疫调节研究。 gzx2962@yahoo.com.cn
  • 基金资助:

    本课题由国家自然科学基金(81000228)资助。

Immunoregulatory function of fetal bone marrow-derived mesenchymal stem cells on human Th17 cells

Guo Zhen-xing1, Zheng Cui-ling2, Chen Zhen-ping3, Dong Wen-chuan1, Yang Ren-chi4   

  1. 1Department of Hematology/Oncology, First Hospital of Tsinghua University, Beijing  100016, China
    2Department of Clinical Laboratory, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing  100021, China
    3Hematology Center, Beijing Children’s Hospital, Beijing  100045, China
    4State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin  300020, China
  • Received:2011-02-28 Revised:2011-03-28 Online:2011-08-06 Published:2011-08-06
  • About author:Guo Zhen-xing☆, Doctor, Department of Hematology/ Oncology, First Hospital of Tsinghua University, Beijing 100016, China gzx2962@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 81000228*

PDF

347

被引次数

3

摘要：

背景：众多研究表明间充质干细胞能发挥免疫调节功能，抑制T细胞增殖。
目的：观察胚胎骨髓来源间充质干细胞对人Th17细胞的调节作用。
方法：将人胚胎骨髓间充质干细胞与正常人外周血单个核细胞或CD4+ T细胞以1∶10比例共培养4 d，以单个核细胞或CD4+T细胞单独培养为对照。应用实时定量PCR检测细胞白细胞介素17 mRNA表达，酶联免疫吸附试验检测细胞上清中白细胞介素17蛋白水平，流式细胞术检测Th17细胞数量。
结果与结论：胚胎骨髓来源间充质干细胞与单个核细胞共培养组白细胞介素17 mRNA表达水平明显高于单个核细胞组(P < 0.01)。与此一致的是，胚胎骨髓来源间充质干细胞与单个核细胞或CD4+T细胞共培养组细胞上清中白细胞介素17蛋白水平明显高于单个核细胞组、CD4+ T细胞组(P < 0.05，P < 0.01)。胚胎骨髓来源间充质干细胞与CD4+ T细胞共培养组Th17细胞数量明显高于CD4+ T细胞组(P < 0.01)，但胚胎骨髓来源间充质干细胞本身并不表达白细胞介素17。表明胚胎骨髓来源间充质干细胞可促进人Th17细胞增殖。

关键词: 胚胎, 骨髓, 间充质干细胞, Th17细胞, 免疫调节

Abstract:

BACKGROUND: Many studies have demonstrated mesenchymal stem cell (MSC) can regulate the immune function and inhibit proliferation of T cell.
OBJECTIVE: To investigate the regulatory function of fetal bone marrow-derived mesenchymal stem cells (FBM-MSCs) on human Th17 cells.
METHODS: FBM-MSCs were cocultured with human peripheral blood mononuclear cells (PBMCs) or CD4+ T cells at 1:10 ratio from healthy donors for 4 days. Single culture of mononuclear cells or CD4+ T cells were as controls. The expression of interleukin-17 (IL-17) mRNA was detected by real-time PCR, IL-17 protein was assayed by enzyme-labeled immunosorbent assay (ELISA), and quantity of Th17 cells was observed by flow cytometry.
RESULTS AND CONCLUSION: The level of IL-17mRNA in FBM-MSC cocultured with PBMC (FBM-MSC/PBMC) group was significantly higher than that in PBMC cultured alone. Meanwhile, IL-17 expression in supernatant in FBM-MSC/PBMC/ CD4+ was significantly higher than that in PBMC and CD4+ T cells groups ( P < 0.05, P < 0.01). The number of FBM-MSCs/CD4+ T cells group was significantly more than CD4+ T cells group ( P < 0.01). However, FBM-MSCs did not express IL-17 protein. FBM-MSCs can promote the proliferation of Th17 cells.

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