中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1232-1236.doi: 10.3969/j.issn.1673-8225.2011.07.020

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

有氧运动对小鼠骨骼肌结节性硬化复合物蛋白2基因表达及胰岛素受体底物1丝氨酸磷酸化的影响

杨风英1，牛燕媚1，刘彦辉2，陈  霓1，王家仲1，傅  力1，2   

  1. 1 天津医科大学康复与运动医学系，天津市 300070
    2 天津体育学院健康与运动科学系，天津市 300381
  • 收稿日期:2010-09-06 修回日期:2010-12-07 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 傅力，博士，博士生导师，教授，天津医科大学康复与运动医学系，天津市 300070；天津体育学院健康与运动科学系，天津市 300381 lifu@tijmu.edu.cn
  • 作者简介:杨风英☆，女，1979年生，山东省人，汉族，天津医科大学在读博士，讲师，主要从事代谢性疾病的运动康复方面的研究工作。 nini@tijmu.edu.cn
  • 基金资助:

    课题受国家自然科学基金(30871213)天津市应用基础及前沿技术重点研究计划资助项目(09JCZDJC17400)资助。

Effect of aerobic exercise on the expression of tuberous sclerosis complex-2 and insulin receptor substrate 1 serine phosphorylation of skeletal muscle in mice

Yang Feng-ying1, Niu Yan-mei1, Liu Yan-hui2, Chen Ni1, Wang Jia-zhong1, Fu Li1,2   

  1. 1Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin  300070, China
    2Department of Health and Exercise Science, Tianjin Institute of Physical Education, Tianjin  300381, China
  • Received:2010-09-06 Revised:2010-12-07 Online:2011-02-12 Published:2011-02-12
  • Contact: Fu Li, Doctor, Doctoral supervisor, Professor, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China; Department of Health and Exercise Science, Tianjin Institute of Physical Education, Tianjin 300381, China lifu@tijmu.edu.cn
  • About author:Yang Feng-ying☆, Studying for doctorate, Lecturer, Department of Rehabilitation and Sports Medicine, Tianjin Medical University, Tianjin 300070, China nini@tijmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30871213*; the Applied Basic Research and Leading Technology Projects, No. 09JCZDJC17400*

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摘要：

背景：研究表明，胰岛素受体底物蛋白1(insulin receptor substrate 1，IRS1)的丝氨酸磷酸化和结节性硬化复合物蛋白2的表达及哺乳动物雷帕霉素靶蛋白/核糖体S6激酶1信号通路的异常可诱导机体产生胰岛素抵抗。
目的：观察有氧运动对小鼠骨骼肌结节性硬化复合物蛋白2基因表达及IRS1丝氨酸磷酸化的影响。
方法：将C57BL/6小鼠随机分为安静组和运动组，运动组进行6周的75%VO2max强度的有氧跑台运动，安静组不做任何干预。采用RT-PCR，Western blot和免疫荧光组织化学染色等方法检测各组大鼠骨骼肌结节性硬化复合物蛋白2，IRS1，pIRS1-Ser307和pIRS1-Ser636/639的表达和定位。
结果与结论：运动组结节性硬化复合物蛋白2 mRNA及蛋白表达高于安静组(P < 0.05)，两组间胰岛素受体底物蛋白1 mRNA及蛋白表达差异无显著性意义。运动组胰岛素受体底物蛋白1的丝氨酸307和丝氨酸636/639位点的磷酸化明显低于安静组(P< 0.05)。结果提示，有氧运动可增强骨骼肌组织结节性硬化复合物蛋白2基因表达，进而抑制胰岛素受体底物蛋白1的丝氨酸磷酸化。

关键词: 有氧运动, 结节性硬化复合物蛋白2, 胰岛素受体底物蛋白1, 磷酸化, 组织构建

Abstract:

BACKGROUND: Studies demonstrated that, expressions of insulin receptor substrate 1 (IRS1) serine phosphorylation and tuberous sclerosis complex-2 (TSC2) and abnormity of mammalian target of rapamycin (mTOR)/S6K1 signaling pathway play an important role in the agitation of insulin resistance.
OBJECTIVE: To study the effects of aerobic exercise on TSC2 and IRS1 serine phosphorylation activity of skeletal muscle in mice.
METHODS: C57BL/6 mice were randomly divided into the control group and exercise group. Mice in the exercise group were acclimatized to the motorized treadmill by running at the intensity of 75%VO2max, for 6 weeks. No intervention was performed in the control group. The expression and location of TSC2, IRS1, IRS1-Ser307 and IRS1-Ser636/639 in mouse skeletal muscle were measured by RT-PCR, Western blot, and immunofluorescence.
RESULTS AND CONCLUSION: The expression of TSC2 mRNA and protein of the exercise group were significantly increased compared with the control group (P < 0.05). There were no significant difference between two groups in the expression of IRS1 mRNA and protein. And the expression of pIRS1-Ser307 and pIRS1-Ser636/639 protein was significantly decreased in the exercise group compared with the control group (P < 0.05). The aerobic exercise probably agitate insulin pathway by enhancing the expression of TSC2 which attenuating the activity of IRS1-Ser307 and IRS1-Ser636/639 phosphorylation. 

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