中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (6): 1015-1019.doi: 10.3969/j.issn.1673-8225.2011.06.015

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

干细胞相关因子在大肠癌组织及SW620细胞株的表达

李秋娴1，黄颖峰2，魏亚明3，王晓华1，罗宏图3，杨邵宇3，许艳丽3，黄于艺1，叶  亨1   

  1. 1广州医学院生化教研室，广东省广州市  510180
    2 广州市第一人民医院普外科，广东省广州市  510180
    3 广州市临床医学研究所，广州市第一人民医院，广东省广州市  510180
  • 收稿日期:2010-08-19 修回日期:2010-11-20 出版日期:2011-02-05 发布日期:2011-02-05
  • 通讯作者: 黄颖峰，硕士，主任医师，广州市第一人民医院普外科，广东省广州市 510180 huangyinfeng@yahoo.com.cn 魏亚明，博士，研究员，广州市临床医学研究所，广州市第一人民医院，广东省广州市510180 weiyaming@163.com
  • 作者简介:李秋娴★，女，1983年生，广东省清远市人，汉族，广州医学院在读硕士，主要从事分子生物学方面的研究。 178248744@qq.com
  • 基金资助:

    广东省医学科研基金(A2008517)。课题名称：消化道肿瘤患者外周血中癌细胞、癌干细胞检测分离与临床应用；广州市科技计划项目(2008J1-C171)。课题名称：大肠癌实体与外周血癌干细胞分离及其Bmi-1基因表达调控。

Expression of stem cell-associated genes in colorectal cancer and SW620 cell line

Li Qiu-xian1, Huang Ying-feng2, Wei Ya-ming3, Wang Xiao-hua1, Luo Hong-tu3, Yang Shao-yu3, Xu Yan-li3, Huang Yu-yi1, Ye Heng1   

  1. 1Biochemistry Teaching and Research Section, Guangzhou Medical College, Guangzhou  510180, Guangdong Province, China
    2Department of General Surgery, Guangzhou Municipal First People’s Hospital, Guangzhou  510180, Guangdong Province, China
    3Guangzhou Institute of Clinical Medicine, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China
  • Received:2010-08-19 Revised:2010-11-20 Online:2011-02-05 Published:2011-02-05
  • Contact: Huang Ying-feng, Master, Chief physician, Department of General Surgery, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China huangyinfeng@yahoo.com.cn Wei Ya-ming, Doctor, Researcher, Guangzhou Institute of Clinical Medicine, Guangzhou Municipal First People’s Hospital, Guangzhou 510180, Guangdong Province, China weiyaming@163.com
  • About author:Li Qiu-xian★, Studying for master’s degree, Biochemistry Teaching and Research Section, Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China 178248744@qq.com
  • Supported by:

    the Medical Scientific Research Foundation of Guangdong Province, No. A2008517*; the Science and Technology Project of Guangzhou City, No. 2008J1-C171*

PDF

569

被引次数

10

摘要：

背景：肿瘤细胞有多种干细胞标记的表达，深入研究其表达规律有助于揭示肿瘤发病机制、完善肿瘤干细胞理论。
目的：检测大肠癌实体组织中干细胞相关分子标记CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1与癌旁组织的表达差异，以及CD133免疫磁珠分选阳性与阴性SW620细胞中上述基因的表达差异。
方法：选临床病理诊断清楚的29例大肠癌组织标本提取RNA，RT-PCR检测CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1在大肠癌组织与癌旁对照组织中的表达。CD133免疫磁珠分选SW620，提取CD133细胞与CD133阴性细胞RNA，RT-PCR检测分选后上述基因的表达差异。
结果与结论：29例标本均诊断为低、中分化腺癌或黏液腺癌，干细胞相关分子标记癌组织与癌旁组织表达灰度相对值癌组织表达均高于癌旁组织(P < 0.05)。RT-PCR检测分选后CD133+细胞CD133,CD166,Oct4,Sox2,C-myc,Klf4,Bmi-1表达，结果均高于CD133-细胞。提示，CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1相关干细胞标记可作为大肠癌肿瘤干细胞标记并有望用于诊断检测。

关键词: 大肠癌, 肿瘤干细胞, CD133, CD166, Oct4, Sox2, Klf4, C-myc, Bmi-1

Abstract:

BACKGROUND: Tumor cells always show some stem cell markers. The expression of these markers will help us to understand the mechanism of tumorgenesis and to perfect the theory of tumor stem cells.
OBJECTIVE: To investigate the expression of stem cell-related molecules markers CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 in colorectal cancer tissue, the difference compared with the expression of adjacent tissues, as well as the difference in expression of above-described genes in sorted CD133+ SW620 cell line.
METHODS: RNA was extracted from cancer tissues of 29 colon cancer tissue specimens. The expression of CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 was detected by RT-PCR in colon cancer tissues and adjacent tissues. CD133+ SW620 cells were sorted, and RNA was extracted from CD133+ cells and CD133- cells. The different expressions of above genes were detected using RT-PCR.
RESULTS AND CONCLUSION: All samples were proved to be poorly/moderately differentiated adenocarcinoma or myxoadenocarcinoma in 29 cases. The relative quantity expressions of the stem cell-related molecules markers in the cancer tissue were higher than adjacent tissues (P < 0.05). RT-PCR indicated that stem cell associated genes expressions (CD133, CD166, Oct4, Sox2, C-myc, Klf4, Bmi-1) in CD133+ sorted cells were higher than that in CD133- ones. These suggested that CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 were used in colorectal cancer diagnosis.

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