中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (5): 891-893.doi: 10.3969/j.issn.1673-8225.2011.05.031

• 器官移植学术探讨 academic discussion of organ transplantation • 上一篇    下一篇

大鼠肾移植1 000例经验总结

李占清1，2，关晓海1，2，王世军3，陈  晶1，2，伊  雪1,2，邬鹏宇1，肖  志2，Nickkholgh Arash2，Bruns Helge2，Hoffmann Katrin2，Schemmer Peter2   

  1. 1华北煤炭医学院附属医院，河北省唐山市  063000
    2海德堡大学移植中心，德国；3德国癌症研究中心，德国
  • 收稿日期:2010-06-17 修回日期:2010-09-21 出版日期:2011-01-29 发布日期:2011-01-29
  • 通讯作者: Schemmer Peter，教授，海德堡大学普外和移植中心副主任，肝脏移植中心主任，德国
  • 作者简介:李占清☆，男，1965年生，河北省唐山市人，汉族，2009年海德堡大学毕业，博士，主任医师，教授，主要从事固体器官移植的应用基础研究，冠状动脉搭桥材料的应用基础研究。 zhanqingli2006@yahoo.com

Kidney transplantation in rats: Experience of 1 000 cases

Li Zhan-qing1,2, Guan Xiao-hai1,2, Wang Shi-jun3, Chen Jing1,2, Yi Xue1,2, Wu Peng-yu1, Xiao Zhi2, Nickkholgh Arash2, Bruns Helge2, Hoffmann Katrin2, Schemmer Peter2   

  1. 1Affiliated Hospital of North China Coal Medical College, Tangshan  063000, Hebei Province, China
    2Department of General and Transplantation Surgery, Ruprecht-Karls-
    University, Heidelberg, Germany; 3German Cancer Research Center (DKFZ), Heidelberg, Germany
  • Received:2010-06-17 Revised:2010-09-21 Online:2011-01-29 Published:2011-01-29
  • Contact: Peter Schemmer, Professor, Chief of the Section of Liver Surgery, Vice Chief of Transplantation Surgery, Department of General and Transplantation Surgery, Ruprecht-Karls-University, Heidelberg, Germany
  • About author:Li Zhan-qing☆, Doctor, Chief physician, Professor, Affiliated Hospital of North China Coal Medical College, Tangshan 063000, Hebei Province, China zhanqingli2006@ yahoo.com

PDF

375

被引次数

18

摘要：

目的：对大鼠肾移植进行经验总结以指导实验与临床研究。
方法：作者在2004-01/2009-07海德堡大学移植中心期间，采用大鼠肾动、静脉与腹主动脉、腔静脉行端侧吻合。对于急性肾移植模型，输尿管直接植入膀胱内；对于慢性肾移植模型，输尿管采用端端吻合法。术中直接切除对侧肾脏，完成大鼠肾移植1 000例，并对其进行总结。
结果：只要供体肾脏冲洗良好，吻合时暖缺血时间小于30 min，手术时间60 min左右，吻合技术娴熟，移植成功率达98%以上。
结论：该实验模型安全、方便、可行。肾移植成功的关键是手术技术。

关键词: 大鼠, 肾移植, 动物模型, 成功率, 器官移植

Abstract:

OBJECTIVE: To review and analyze our experience in rat kidney transplantation which provides guidance for experimental and clinical studies.
METHODS: Between January 2004 and July 2009, about 1000 rat kidneys were transplanted in the research facilities of the Department of General and Transplantation Surgery, Ruprecht-Karls-University, Germany. Briefly, the renal grafts’ artery and vein were both anastomosed in an end-to-side technique with running sutures to the recipients’ abdominal aorta and vena cava, respectively. In the acute and chronic kidney transplantation model the ureter was implanted either directly into the bladder or an end-to-end ureteral anastomosis was performed, respectively. Recipients were nephrectomized bilaterally.
RESULTS: Flushed donor kidneys were transplanted with a warm ischemia time of less than 30 minutes and a total operative time of about 60 minutes. The procedure was successful in more than 98% of cases.
CONCLUSION: The kidney transplantation model described here is safe, convenient and feasible; however, microsurgical technique requires a skillful surgeon who is the key factor for successful surgical research.

中图分类号: