中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (50): 9391-.doi: 10.3969/j.issn.1673-8225.2010.50.020

• 组织构建与中医药 • 上一篇    下一篇

补肾益气中药与膝骨性关节炎关节液中白细胞介素1及白细胞介素6的表达>

袁普卫，刘德玉，杨镇源，楚向东，郝阳泉，朱超，屈强   

  1. 陕西中医学院骨伤研究所，陕西省咸阳市 712000
  • 出版日期:2010-12-10 发布日期:2010-12-10
  • 作者简介:袁普卫☆，男，1979年生，医学博士，副教授，副主任医师，主要从事中西医结合骨科方面的研究。 spine_surgeon@163.com
  • 基金资助:

    陕西省教育厅专项科研计划(09JK411)；陕西省教育厅重点学科及国家中医管理局重点建设专科专项基金。

Effect of Chinese medicine replenishing kidney and invigorating Qi on expression of interleukin-1 and interleukin-6 in synovial fluid of knee osteoarthritis 
 

Yuan Pu-wei, Liu De-yu, Yang Zhen-yuan, Chu Xiang-dong, Hao Yang-quan, Zhu Chao, Qu Qiang   

  1. Research&nbsp;Institute&nbsp;of&nbsp;Orthopedics&nbsp;and&nbsp;Traumatology,&nbsp;Shaanxi&nbsp;University&nbsp;of&nbsp;Chinese&nbsp;Medicine,&nbsp;Xianyang&nbsp;&nbsp;712000,&nbsp;Shaanxi&nbsp;Province,&nbsp;China</div>
  • Online:2010-12-10 Published:2010-12-10
  • About author:Yuan Pu-wei☆, Doctor, Associate professor, Associate chief physician, Research Institute of Orthopedics and Traumatology, Shaanxi University of Chinese Medicine, Xianyang 712000, Shaanxi Province, China spine_surgeon@163.com
  • Supported by:

    the Scientific Research Plan Projects of Shaanxi Education Department, No. 09JK411*

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摘要：

背景：对骨性关节炎的治疗目前尚无逆转和阻断的有效方法，只能缓解症状和延缓病变的进程。
目的：探讨补肾益气法中药预防家兔膝骨性关节炎的作用机制。
方法：4月龄健康日本长耳大白兔随机分为对照组、模型组、中药大、中、小剂量组、盐酸氨基葡萄糖胶囊对照组。造成兔膝骨性关节炎模型同时分别给予不同剂量中药及盐酸氨基葡萄糖胶囊灌胃4周，应用酶联免疫吸附法测定关节液中白细胞介素1及白细胞介素6的变化，同时取关节软骨行大体及光镜检查。
结果与结论：治疗后动物关节软骨有不同程度的色泽变化，骨赘、骨囊肿形成，软骨关节面出现浅表性糜烂，部分标本软骨缺损深达软骨中层，出现软骨剥脱，但程度明显降低。中药大、中、小剂量组及盐酸氨基葡萄糖胶囊对照组关节液中白细胞介素1及白细胞介素6水平均显著降低(P < 0.05)，且随用药剂量增大，兔关节液中白细胞介素1及白细胞介素6水平降低幅度增强。提示补肾益气法中药可能通过降低兔关节液中白细胞介素1及白细胞介素6水平，来达到预防骨性关节炎的发生。

关键词: 补肾益气法, 骨性关节炎, 预防, 白细胞介素1, 白细胞介素6

Abstract:

BACKGROUND: Osteoarthritis can be released or delayed rather than cured at present.
OBJECTIVE: To explore the mechanism of Chinese medicine with replenishing kidney and invigorating Qi on prevention of osteoarthritis.
METHODS: Long-eared white rabbits, aged 4 months, were randomly divided into six groups, control, model, traditional Chinese medicine high-, middle-, and low-dose and glucose-amino acid hydrochloride capsules groups. All animals were established osteoarthritis model and treated by traditional Chinese medicine with different doses or glucose-amino acid hydrochloride capsules for 4 weeks. The variations of interleukin-1 and interleukin-6 in joint fluid extraction were determined by ELISA. Gross and light microscope observations were performed on articular cartilages.
RESULTS AND CONCLUSION: The articular cartilage had varying degree degeneration, osteophyte and bone cysts formed and defects deep into middle zone of parts of cartilages after treatment. Compared with the control group, the synovial fluid interleukin-1 and interleukin-6 levels were obviously decreased in the traditional Chinese medicine high-, middle-, and low-dose and glucose-amino acid hydrochloride capsules groups (P < 0.05), and the decreased degree became greater with dose increasing. Chinese medicine with replenishing kidney and invigorating Qi can prevent osteoarthritis via reducing synovial fluid interleukin-1 and interleukin-6 levels.

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