中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9239-.doi: 10.3969/j.issn.1673-8225.2010.49.027

• 干细胞基础实验 • 上一篇    下一篇

改良法培养人牙髓间充质干细胞及其向神经样细胞的分化

李  远1，2，关方霞3，李  季4，杨  波1，2，胡  祥5，6，杜  英7，谷晨熙8，雷宁静3，王晓薇9   

  1. 1郑州大学第一附属医院神经外科，河南省郑州市 450052；2河南省高等学校临床医学重点学科开放实验室，河南省郑州市  450052；  3郑州大学生物工程系，河南省郑州市  450001；4郑州大学第一附属医院口腔科，河南省郑州市 450052；5江苏省干细胞与生物治疗公共技术服务平台，江苏省泰州市  225300；6深圳市北科细胞工程研究所，广东省深圳市 518000；7郑州大学基础医学院微生物与免疫学教研室，河南省郑州市 450052；8郑州大学临床医学系，河南省郑州市 450001；9湖南师范大学生命科学学院，湖南省长沙市  410081
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 杨波，硕士，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市 450052 yangbo96@126.com
  • 作者简介:李远☆，男，1983年生，河南省郑州市人，汉族，郑州大学医学院在读博士，医师，主要从事间质干细胞和脑肿瘤干细胞方面的研究。Liyuans889@Gmail.com 并列第一作者：关方霞，女，1969年生，陕西省西安市人，汉族，1999年山东医科大学毕业，博士，教授，主要从事间质干细胞与肿瘤干细胞方面的研究。guanfangxia@126.com

Differentiation of modified method cultured human dental pulp mesenchymal stem cells into neuron-like cells

Li Yuan 1, 2, Guan Fang-xia3, Li Ji4, Yang Bo 1,2, Hu Xiang 5,6, Du Ying7, Gu Chen-xi8, Lei Ning-jing 3, Wang Xiao-wei9   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou  450052, Henan Province, China; 3Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 4Department of Stomatology, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052 Henan Province, China; 5Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, Taizhou  225300, Jiangsu Province, China; 6Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China; 7Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 8Department of Clinical Medicine, Zhengzhou University, Zhengzhou  450001, Henan Province, China; 9College of Life Science, Hunan Normal University, Changsha  410081, Hunan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yang Bo, Master, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Li Yuan☆, Studying for doctorate, Physician, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open and Key Laboratory of Clinical Medicine at Henan Universities, Zhengzhou 450052, Henan Province, China Liyuans889@Gmail.com Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com Li Yuan and Guan Fang-xia contributed equally to this article
  • Supported by:

    the Zhengzhou University ‘211 project-phase Ⅲ’; the Jiangsu Public Technology Service Platform of Stem Cells and Biotherapy, No. BM2008146*

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摘要：

背景：应用改良组织原位培养法后，牙髓间充质干细胞的提取成功率显著提高，但鲜有文献报道其与酶消化法提取干细胞生物学特性的异同。
目的：观察改良组织原位培养法与传统酶消化法所获得的牙髓间充质干细胞向神经样细胞分化上的差异。
方法：将同一组织来源的标本随机分成两组，分别用改良的组织原位培养法及常规的酶消化法提取人牙髓间充质干细胞，通过免疫组化、流式细胞检测及RT-PCR等比较观察其生长情况、抗原表达及向神经样细胞分化能力。
结果与结论：改良法原位培养组分离牙髓间充质干细胞与常规分离培养组分离的干细胞在形态学表现，抗原表达及神经向分化能力上无明显区别，但简化了操作流程，且所得细胞数量大。

关键词: 改良法, 原位培养, 干细胞, 牙髓, 神经分化

Abstract:

BACKGROUND: The success rate of dental pulp stem cells (DPSCs) which isolated by the modified cultured method of tissue is elevated. However, few reports have concerned the difference in biological features between DPSCs and stem cells extracted by emzyme digestion method.
OBJECTIVE: To observe the difference in the differentiation of DPSCs isolated by the new method and isolated by traditional method into neuron-like cells.
METHODS: The specimen of the same tissue was randomly divided into two parts: one is isolated by the modified cultured method of tissue (A group), another is isolated by the traditional method (B group). Growth, antigen expression and differentiation of DPSCs into neuron-like cells were observed and compared using immunohistochemical method, flow cytometry and reverse transcription-polymerase chain reaction.
RESULTS AND CONCLUSION: There were no distinct changes in morphology expression, antigen ability, differentiation ability into neurons between A group and B group. However, A group had simple process and obtained a large quantity.

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