中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (47): 8809-8813.doi: 10.3969/j.issn.1673-8225.2010.47.017

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

5-氟尿嘧啶磁性纳米粒的制备及性能

王  津，林友文，王明娜   

  1. 福建医科大学药学院，福建省福州市 350004
  • 出版日期:2010-11-19 发布日期:2010-11-19
  • 作者简介:王津★，女，1975年生，福建省宁德市人，汉族，1998年吉林大学毕业，硕士，讲师，主要从事聚合物载体改性及应用研究。wangjinfj@sina.com
  • 基金资助:

    福建省教育厅资助项目(JA09123)。

Preparation and properties of 5-Fluorouracil magnetic nanoparticles

Wang Jin, Lin You-wen, Wang Ming-na   

  1. Faculty of Pharmacy, Fujian Medical University, Fuzhou   350004, Fujian Province, China
  • Online:2010-11-19 Published:2010-11-19
  • About author: Wang Jin★, Master, Lecturer, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, Fujian Province, China wangjinfj@sina.com
  • Supported by:

     a grant by Fujian Provincial Education Bureau, No. JA09123*

PDF

520

被引次数

12

摘要：

背景：磁性载药微粒在外加磁场作用下，能实现定向治疗作用，减少全身毒副作用，同时作为缓释载体，能减少药物的频繁给药，达到有效治疗目的。
目的：制备5-氟尿嘧啶的磁性纳米粒，评价微球性能。
方法：以海藻酸钠和壳聚糖作为壁材，5-氟尿嘧啶为模型药物，Span80为乳化剂，液体石蜡为分散介质，乳化-复凝聚法制备磁性纳米粒。并从外观、稳定性、磁响应性、结构、溶胀实验及体外释放实验等多方面考察纳米粒性能。
结果与结论：所制5-氟尿嘧啶的磁性纳米粒形态良好，均匀圆整，分散性较好，粒径在100~300 nm，具有较好稳定性和磁响应性。考察Fe3O4用量的影响，发现随着Fe3O4用量的增加，磁响应性增强，但载药量下降。红外谱图说明微粒中包裹磁性物质Fe3O4，及5-氟尿嘧啶与壁材之间产生相互作用。将5-氟尿嘧啶磁性纳米粒分别浸渍在蒸馏水，0.9%NaCl溶液和磷酸盐缓冲液(pH=7.4)中，结果蒸馏水吸水速度最快且溶胀率最大，磷酸盐缓冲液(pH=7.4)吸水程度最小且溶胀率最小。微粒的缓释性能良好，50 h内释放的药物占总含药量的53.20%。

关键词: 磁性, 纳米粒, 5-氟尿嘧啶, 壳聚糖, 海藻酸钠, 缓释

Abstract:

BACKGROUND: Within a magnetic field, magnetic drug-loaded nanoparticles can implement targeted therapy, reduce systemic side effect. Also serving as a release carrier, magnetic drug microspheres can decrease administration frequency and achieve effective treatment.
OBJECTIVE: To prepare 5-Fluorouracil magnetic nanoparticles, and to estimate nanoparticles’ performance.
METHODS: Nanometer-sized magnetic release particles were prepared by an emulsifying-complex coacervation method with alginate-chitosan as the coating material, 5-Fluorouracil as model drug, Span80 as emulsifier, liquid paraffin as disperse medium. The shape, stability, magnetic responsiveness, structures, swelling action, release action in vitro of nanoparticles were investigated.
RESULTS AND CONCLUSION: The 5-Fluorouracil nanoparticles were spherical shape with a regular surface, fine dispersibility at a diameters of 100-300 nm. They have good stability and good magnetic responsiveness. To investigate the influence of Fe3O4 dosage, with the increase of Fe3O4 dosage, the magnetic responsiveness increased, while the loading capacity increased. The infrared results showed that the nanoparticles encapsulated with Fe3O4, and 5-Fluorouracil interacted with coating materials. The 5-Fluorouracil nanoparticles were immersed in three mediators of distilled water, 0.9% NaCl and phosphate buffer saline (pH=7.4), the hydrating rate and swelling ratios in distilled water were the highest, while the lowest in phosphate buffer saline (pH=7.4). The sustained-releasing ability of nanoparticles was well, and the releasing content counted for 53.20% of total drug within 50 hours.

中图分类号: