中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (47): 8795-8799.doi: 10.3969/j.issn.1673-8225.2010.47.014

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

羟基磷灰石/胶原蛋白复合支架上骨髓间充质干细胞的增殖与分化

宋  芹1 ，颜  军1，郭晓强1，姚  倩1，郁小兵○2,3   

  1. 1成都大学生物产业学院，四川省成都市   610106；2日本高研株式会社生物研究所，日本东京1150051；3新乡医学院组织再生重点实验室，河南省新乡市  453000
  • 出版日期:2010-11-19 发布日期:2010-11-19
  • 通讯作者: 郁小兵，教授，博士，日本高研株式会社生物研究所，日本东京1150051；新乡医学院组织再生重点实验室，河南省新乡市 453000
  • 作者简介:宋芹☆，女，1982年生，四川省成都市人，汉族，2009年成都中医药大学毕业，博士，讲师，主要从事生物医学工程研究。 sq82802@hotmail.com

Proliferation and differentiation of bone marrow mesenchymal stem cells cultured on hydroxyapatite/collagen composite scaffolds

Song Qin1, Yan Jun1, Guo Xiao-qiang1, Yao Qian1, Yu Xiao-bing○2,3   

  1. 1 College of Biotechnology Industry, Chengdu University, Chengdu   610106, Sichuan Province, China; 2 Koken Bioscience Institute, Tokyo   1150051, Japan; 3 Laboratory of Tissue Regeneration, Xinxiang Medical College, Xinxiang   453003, Henan Province, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Yu Xiao-bing, Professor, Doctor, Koken Bioscience Institute, Tokyo 1150051, Japan; Laboratory of Tissue Regeneration, Xinxiang Medical College, Xinxiang 453003, Henan Province, China
  • About author:Song Qin☆, Doctor, Lecturer, College of Biotechnology Industry, Chengdu University, Chengdu 610106, Sichuan Province, China sq82802@hotmail.com

PDF

458

被引次数

20

摘要：

背景：最近胶原蛋白作为细胞体外三维立体支架材料被大量用于组织工程，但胶原蛋白支架材料机械强度低，很难承受较大外力，为此将胶原蛋白与羟基磷灰石制成复合支架材料，探索复合支架的生物相容性和生物活性。
目的：验证大鼠骨髓间充质干细胞在羟基磷灰石/胶原蛋白复合支架上的增殖与分化情况。
方法：贴壁法分离SD大鼠骨髓间充质干细胞，分别在平板、胶原支架、羟基磷灰石/胶原蛋白复合支架上，观察3种条件下细胞的生长状况，四甲基偶氮唑盐法检测其增殖情况。培养14，21 d后，考察细胞碱性磷酸酶活性及胶原蛋白分泌量。
结果与结论：发现骨髓间充质干细胞在羟基磷灰石/胶原蛋白复合支架上铺展良好，其增殖率显著高于纯胶原蛋白支架上培养的细胞，并高于平板培养细胞。骨髓间充质干细胞在羟基磷灰石/胶原蛋白复合支架上的碱性磷酸酶活性及胶原蛋白分泌量明显高于纯胶原蛋白支架，高于平板培养。结果提示，羟基磷灰石/胶原蛋白复合材料能促进骨髓间充质干细胞的增殖和分化，并诱导骨髓间充质干细胞向成骨细胞分化。

关键词: 胶原蛋白, 羟基磷灰石, 骨组织工程, 骨髓间充质干细胞, 支架

Abstract:

BACKGROUND: Presently collagen as cell three-dimensional scaffold in vitro, has been mainly used in tissue engineering. But collagen scaffolds has low mechanical strength, and weak fatigue resistance. Therefore hydroxyapatite/collagen composite scaffolds are prepared to explore the biocompatibility and bioactivity of the composite scaffold.
OBJECTIVE: To investigate the proliferation and differentiation of bone marrow mesenchymal stem cells (MSCs) cultured on hydroxyapatite/collagen composite scaffolds.
METHODS: MSCs were isolated from SD rats using adherence method and inoculated into the dish, collagen scaffold and hydroxyapatite/collagen composite scaffold respectively. The cellular growth under three conditions was observed, the cellular proliferation was assessed using MTT assay. The differentiation of MSCs was characterized by alkaline phosphatase activity and the secretion of collagen at 14 and 21 days after the culture.
RESULTS AND CONCLUSION: The MSCs well spread on the hydroxyapatite/collagen composite scaffolds, and showed a higher proliferation rate than that cultured on the collagen scaffold and dish. The quantitative examination of alkaline phosphatase activity and secretion of collagen indicated that the cells cultured on the hydroxyapatite/collagen composite scaffolds expressed a higher level than that on the collagen scaffold and dish. The hydroxyapatite/collagen composite scaffold can promote the differentiation and proliferation of MSCs, and induce MSCs differentiate into osteoblasts.

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