中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8608-.doi: 10.3969/j.issn.1673-8225.2010.46.014

• 组织构建实验造模 • 上一篇    下一篇

不同程度大鼠冻伤模型的构建

王鹏，李剑
  

  1. 南昌大学第二附属医院江西省分子医学重点实验室，江西省南昌市  330006
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 通讯作者: 李剑，博士，研究员，南昌大学第二附属医院江西省分子医学重点实验室，江西省南昌市 330006 jeniferjian@gmail.com
  • 基金资助:

    国家十一五支撑计划项目(2008BAI68B06)，国家自然科学基金(30760261)。课题名称：极端气候条件下创伤疾病研究、探索无血清培养条件下人胚胎干细胞体外定向诱导组织工程皮肤。

Construction of rat models with frostbite to varying degrees

Wang Peng, Li Jian   

  1. Jiangxi Provincial Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Li Jian, Doctor, Investigator, Jiangxi Provincial Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China jeniferjian@gmail.com
  • Supported by:

    the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period, No. 2008BAI68B06*; the National Natural Science Foundation of China, No. 30760261*

PDF

353

被引次数

15

摘要：

背景：冻伤目前尚无特异治疗方法，良好的冻伤模型对研究冻伤的机制及修复有重要意义。
目的：建立大鼠不同程度皮肤冻伤模型，探讨建立理想冻伤模型的新方法。
方法：将一元钱硬币在液氮罐中冷冻至-196 ℃后紧贴SD大鼠皮肤3，5，10 s， 24 h后分别切取冻伤组织标本行常规苏木精-伊红染色，显微镜下观察组织的病理结构改变。
结果与结论：冻伤3 s所造成的轻度冻伤只破坏了表皮浅层，基底层未发生改变；冻伤5 s所造成的中度冻伤破坏了表皮全层(含基底层)及真皮浅层，但真皮深层及毛囊根部未受损伤；冻伤10 s所造成的重度冻伤破坏了真皮深层及毛囊根部。说明冻伤3 s所造成的轻度冻伤和冻伤5 s所造成的中度冻伤可作为依据建立动物模型。

关键词: 冻伤, 动物模型, 大鼠, 不同程度, 皮肤

Abstract:

BACKGROUND: There is not a specific treating method for frostbite. A frostbite model has great significance in researching the mechanism of frostbite and its repair. OBJECTIVE: To construct rat models with varying degrees frostbite, and to explore a new method for frostbite model preparation.  
METHODS: Coins were immersed into liquid nitrogen to reach -196℃ and then nestled up to the skin of rat for 3, 5 and 10 seconds. Specimens of frostbite tissue were harvested after 24 hours and performed routine hematoxylin-eosin staining. Changes of histopathologic structure after frostbite were also observed under a microscope.
RESULTS AND CONCLUSION: For light frostbite (3 seconds), only superficial epidermis was damaged, underlayer appeared normal. For midrange frostbite (5 seconds), full-thickness of epidermis, including the underlayer, was damaged, however, deeplayer of dermis and hair follicle were intact. For severe frostbite (10 seconds), deeplayer of dermis and hair follicle were damaged wholly. Light frostbite and midrange frostbite could be used as a standard to construct animal models.

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