中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (46): 8568-.doi: 10.3969/j.issn.1673-8225.2010.46.005

• 软骨组织构建 • 上一篇    下一篇

体外培养软骨细胞与生长因子的加入 

陈北北   

  1. 沈阳医学院奉天医院骨外一科，辽宁省沈阳市 110024
  • 出版日期:2010-11-12 发布日期:2010-11-12
  • 作者简介:陈北北，男，1972年生，辽宁省沈阳市人，汉族，1995年沈阳医学院毕业，副教授，主要从事创伤骨科方面的研究。 haiyan556677@163.com
  • 基金资助:

    辽宁省教育厅科学技术研究项目（L2010553）：微机控制应力诱导的软骨细胞复合体组织工程研究。

Effect of growth factors on in vitro cultured articular chondrocytes  

Chen Bei-bei   

  1. First Department of Bone Surgery, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang  110024, Liaoning Province, China
  • Online:2010-11-12 Published:2010-11-12
  • About author:Chen Bei-bei, Associate professor, First Department of Bone Surgery, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang 110024, Liaoning Province, China haiyan556677@163.com
  • Supported by:

     the Science and Technology Research Program of Education Commission of Liaoning Province, No. L2010553*

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摘要：

背景：自体软骨细胞体外培养生长缓慢，极易发生去分化，胰岛素样生长因子和碱性成纤维细胞生成因子可以刺激关节软骨细胞的增殖和分化。
目的：观察应用碱性成纤维细胞生成因子和胰岛素样生长因子1对关节软骨细胞的作用。
方法：采用成人关节软骨细胞体外培养，以碱性成纤维细胞生成因子(10 μg/L)和胰岛素样生长因子1(100 mg/L)对其作用，采用MTT法和免疫细胞化学技术进行观察和评价。
结果与结论：在体外培养软骨细胞时应用碱性成纤维细胞生成因子可以明显促进细胞增殖，但同时发生去分化。在体外培养软骨细胞时应用胰岛素样生长因子1可以明显促进细胞产生细胞外基质，有利于软骨细胞表型的表达，对细胞的增殖作用不明显。提示采用顺序性应用胰岛素样生长因子1和碱性成纤维细胞生成因子对体外培养的软骨细胞进行作用，可以在更短的时间内获得大量的、具有良好细胞表型的软骨细胞。

关键词: 碱性成纤维细胞生长因子, 胰岛素样生长因子1, 软骨细胞, 培养, 体外

Abstract:

BACKGROUND: Articular chondrocytes grow slowly and easily dedifferentiate in vitro. However, insulin-like growth factor (IGF) and basic fibroblast growth factor (bFGF) can stimulate proliferation and differentiation of articular chondrocytes.
OBJECTIVE: To explore the sequential interaction of bFGF and IGF-1 on articular condrocytes culture.
METHODS: Articular chondrocytes was cultured using growth factors, single bFGF (10 μg/L), single IGF-1 (100 mg/L), sequential bFGF and IGF-1 and were managed by MTT and immunocytochemical technique.
RESULTS AND CONCLUSION: bFGF obviously promoted proliferation and dedifferentiation of articular chondrocytes in vitro. IGF-1 promoted proliferation of extracellar matrix and expression of chondrocyte phenotypes, but it failed to facilitate multiplication of chondrocytes. Sequential exertion of bFGF and IGF-1 in the culture medium can harvest abundant proliferative chondrocytes with ideal phenotypes in a limited period of culture time.

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