中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8491-8494.doi: 10.3969/j.issn.1673-8225.2010.45.031

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

细胞永生化与细胞癌变之间关系的研究进展

吴  迪1，贾永峰1，吴  岩2   

  1. 内蒙古医学院， 1病理学中心，  2组胚教研室，内蒙古自治区呼和浩特市 010059
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 贾永峰，博士，教授，内蒙古医学院病理学中心，内蒙古自治区呼和浩特市 010059 yfjia1973@yahoo.com.cn
  • 作者简介:吴迪★，男，1979年生，内蒙古自治区赤峰市人，汉族，内蒙古医学院在读硕士，主要从事肿瘤分子病理学方面的研究。 wudi080101@126.com
  • 基金资助:

    教育部“春晖”计划资助(课题号Z2007-1-01002)，课题名称“乳腺癌和正常乳腺上皮细胞的永生化与癌变关系的研究”。干细胞应用技术创新团队基金 内蒙古自治区应用技术研究与开发资金计划资助(课题号2009)。

Research progress in the relationship between cell immortalization and carcinogenesis

Wu Di1, Jia Yong-feng1, Wu Yan2   

  1. 1 Pathology Center, 2 Department of Histology and Embryology, Inner Mongolia Medical College, Huhhot  010059, Inner Mongolia Autonomous Region, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Jia Yong-feng, Doctor, Professor, Pathology Center, Inner Mongolia Medical College, Huhhot 010059, Inner Mongolia Autonomous Region, China yfjia1973@yahoo.com.cn
  • About author:Wu Di★, Studying for master’s degree, Pathology Center, Inner Mongolia Medical College, Huhhot 010059, Inner Mongolia Autonomous Region, China wudi080101@126.com
  • Supported by:

    the “Spring Sunshine” Project of Education Ministry, No. Z2007-1-01002*; the Stem Cell Application Technique Innovation Team Foundation, the Application Technique Research and Development Fund Project of Inner Mongolia Autonomous Region, No. 2009*

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摘要：

背景：永生化细胞和肿瘤细胞在基因表达水平、蛋白水平及表型水平等方面存在诸多区别和联系，目前对两种细胞之间关系的认识仍存在争议。
目的：分析永生化细胞与肿瘤细胞之间的关系，总结肿瘤细胞生成有关理论的研究进展和前景。
方法：应用计算机检索PubMed数据库和SpringerLink数据库(2001-01/2010-01)，以“cellular immortality，cancer mechanism”为检索词。应用计算机检索中国知网数据库(2001-01/2010-01)，以“细胞永生化、癌变机制”为检索词。
结果与结论：共收集95篇文献，中文35篇，英文60篇。排除发表时间较早、重复及类似研究，最终应用文献41篇。永生化细胞不是肿瘤细胞，普遍认为细胞永生化是细胞癌变过程中的必经阶段，若是如此，肿瘤细胞应易于培养。而实际上肿瘤细胞初代培养时非常困难，因此有的学者就二者之间的关系提出质疑。目前有关肿瘤细胞生成的理论存在非整倍体假说、DNA永生化链假说、肿瘤干细胞假说等。对二者之间关系的进一步研究，有利于永生化细胞的应用及肿瘤的治疗。

关键词: 永生化细胞, 肿瘤细胞, 形成, DNA永生化链, 肿瘤干细胞, 综述文献

Abstract:

BACKGROUND: Many differences and connections have been observed between immortalized cells and tumor cells in the level of gene, protein and phenotypic types. It was some different opinions that exist in the relationship between the two cell types.
OBJECTIVE: To analyze the correlation between immortalized cells and tumor cells, and to summarize research progress and perspective of studying tumor cell production.
METHODS: Pubmed and SpringerLink databases were searched by computer for articles published from January 2001 to January 2010. The key words were “cellular immortality, cancer mechanism”. China National Knowledge Infrastructure database was searched by computer for articles published from January 2001 to January 2010. The key words were “cellular immortality, cancer mechanism”.
RESULTS AND CONCLUSION: A total of 95 relevant articles were collected including 35 Chinese articles and 60 English articles. Finally, 41 articles were used after the earlier publishers and similar repeated studies were excluded. Immortalized cells are not tumor cells. It is generally believe that cells immortalized passages is a necessary step in the process of malignant transformation of cells, if just as it said so, the culture of tumor cells should be easy. In fact, in vitro culture of tumor cells is very difficult; some scholars have questioned the relationships between the two. At present, the theory on the arising of tumor cells exists as follows: aneuploidy hypothesis, DNA immortalized chain hypothesis, cancer stem cell hypothesis and so on. The further study of the relationships between the two is helpful to the application of immortalized cells and beneficial to tumor treatment.

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