中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8473-8475.doi: 10.3969/j.issn.1673-8225.2010.45.026

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

一种简单实用的单细胞分离技术　

黄名威1，陆云飞2，沈桂鑫1，李  涛1   

  1. 1广西医科大学，广西壮族自治区南宁市 530021；2广西医科大学第一附属医院胃肠腺体外科，广西壮族自治区南宁市  530021
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 陆云飞，硕士，主任医师，广西医科大学第一附属医院胃肠腺体外科，广西壮族自治区南宁市 530021
  • 作者简介:黄名威☆，男，1977年生，广西壮族自治区上林县人，汉族，2010年广西医科大学毕业，博士，主治医师，主要从事肿瘤发生学研究。
  • 基金资助:

    广西研究生教育创新计划基金资助项目(2008105981002D33)：乳腺癌MCF7细胞株中干细胞亚群的筛选鉴定及其蛋白质谱分析。

A simple and effective method for single cells separation

Huang Ming-wei1, Lu Yun-fei2, Shen Gui-xin1, Li Tao1　   

  1. 1 Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Surgical Department of Intestines-Stomach and Glandular Organ, First Affiliated Hospital, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autnomous Region, China　
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Lu Yun-fei, Master, Chief physician, Surgical Department of Intestines-Stomach and Glandular Organ, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autnomous Region, China
  • About author:Huang Ming-wei☆, Doctor, Attending physician, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Postgraduate Education Innovation Project of Guangxi Zhuang Autonomous Region, No. 2008105981002D33*

PDF

658

摘要：

背景：基于单细胞水平的研究是干细胞、肿瘤干细胞研究的发展趋势。许多研究者常常因为无法轻易获得足够数量的单细胞而在研究时望而却步。掌握简单有效的单细胞分离技术为准确、定量的单细胞水平研究奠定基础。
目的：介绍一种简单有效的单细胞分离技术。
方法：在显微操作法的基础上改进，利用改制小号的静脉注射静脉注射针及微量移液器组合制成单细胞分离种植装置并采取双手操作法进行单细胞分离。采用染色细胞分离的方法对该技术分离单细胞的效率进行验证。
结果与结论：单细胞分离的成功率达到96.5%。利用该技术分离单细胞具有取材容易、制作简单、操作直观可靠、分离成功率高、易于推广普及的特点。分离的单细胞可直接用于进一步的研究。

关键词: 单细胞, 细胞培养, 流式细胞仪, 单细胞分离

Abstract:

BACKGROUND: Research of stem cell or cancer stem cell at single cell level would be popularity in the near further. Many researchers stop going ahead when they have no idea to obtain lots of single cells in their study. A simple and effective method for single cell separation would be a foundation of exactly and quantitative study at single cell level.
OBJECTIVE: To introduce a simple and effective method for single cell separation.
METHODS: The separation method was improved based on micromanipulate and single cells were separated by using special separation equipment which makes up of micropipettor and the smallest intravenous needle. Success rates of separation were evaluated by separated staining of single cells.
RESULTS AND CONCLUSION: Success rate of single cells separation was 96.5%. The method has many advantages: material was easily to find; the manipulate method was simple, visual, reliable and easy to popularization and application; high success rate. Those single cells which had been separated can be used for further study directly.

中图分类号: