中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8441-8445.doi: 10.3969/j.issn.1673-8225.2010.45.019

• 干细胞转基因表达 transgenic expression in stem cells • 上一篇    下一篇

人巨细胞病毒感染对脐血红系祖细胞发育过程中Hoxb2，Hoxb4基因表达的影响　

黄媚贤，刘文君，郭渠莲，陈军红，施  翰   

  1. 泸州医学院附属医院儿科，四川省泸州市  646000
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 刘文君，博士，主任医师，教授，硕士生导师，泸州医学院附属医院儿科，四川省泸州市 646000 lwjlyfy@yahoo.com.cn
  • 作者简介:黄媚贤★，女，1982年生，福建省南安市人，汉族，2009年泸州医学院毕业，硕士，医师，主要从事小儿血液的研究。目前在泉州医学高等专科学校儿科教研室工作。 313719941@qq.com
  • 基金资助:

    四川省教育厅重点科研基金(2004A058)。

Effects of human cytomegalovirus infection on expressions of Hoxb2 and Hoxb4 gene in the development process of cord blood erythroid progenitor cells

Huang Mei-xian, Liu Wen-jun, Guo Qu-lian, Chen Jun-hong, Shi Han　   

  1. Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou  646000, Sichuan Province, China 　
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Liu Wen-jun, Doctor, Chief physician, Professor, Master’s supervisor, Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China lwjlyfy@yahoo.com.cn
  • About author:Huang Mei-xian★, Master, Physician, Department of Pediatrics, Hospital Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China; Now working at Research Room of Pediatrics, Quanzhou Medical College 313719941@qq.com
  • Supported by:

    the Key Scientific Research Foundation of Department of Education of Sichuan Province, No. 2004A058*

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摘要：

背景：人巨细胞病毒感染可损害造血系统，甚至造成骨髓衰竭。人巨细胞病毒感染抑制红系祖细胞增殖和分化，是否与受染红系祖细胞增殖的基因异常表达有关？
目的：观察人巨细胞病毒和/或全反式维甲酸对人脐血造血干细胞向红系祖细胞定向增殖分化过程进行干预后Hoxb2、Hoxb4基因表达的变化。
方法：取12例正常足月顺产新生儿断脐后的胎盘段脐血，采用造血干细胞体外培养技术及实时荧光定量聚合酶链反应方法，以人巨细胞病毒-AD169和(或)6×10-8 mol/L的全反式维甲酸持续干预人脐血造血干细胞向红系定向增殖分化过程，检测空白组、全反式维甲酸组、人巨细胞病毒组、全反式维甲酸+人巨细胞病毒组在培养第3，7，10天红系祖细胞Hoxb2、Hoxb4基因的表达情况。
结果与结论：各组Hoxb2、Hoxb4基因均在增殖分化的第3天已有表达，第7天表达明显增加，第10天表达最强烈。与空白组相比，人巨细胞病毒组明显下降；与人巨细胞病毒组相比，全反式维甲酸+人巨细胞病毒组的Hoxb2、Hoxb4基因表达量明显增加(P < 0.05)。提示人巨细胞病毒可能通过调控Hoxb2、Hoxb4基因异常表达而引起造血功能异常；Hoxb2、Hoxb4均与红系造血有相关性；6×10-8 mol/L全反式维甲酸能显著上调正常红系祖细胞Hoxb2、Hoxb4基因的表达，也能上调经人巨细胞病毒感染的红系祖细胞Hoxb2、Hoxb4基因的表达。

关键词: 巨细胞病毒, 红系祖细胞, 全反式维甲酸, 实时荧光定量聚合酶链反应, Hoxb2, Hoxb4

Abstract:

BACKGROUND: Human cytomegalovirus (HCMV) infection can damage the hematopoietic system, and even cause bone marrow failure. HCMV infection inhibited erythroid progenitor cell proliferation and differentiation, and whether the proliferation of erythroid progenitor cells associated with abnormal expression of genes?
OBJECTIVE: To investigate the expressions of the Hoxb2 gene and Hoxb4 gene after the differentiation and proliferation of the hematopoietic stem cells to the colony forming erythroid progenitor cells (CFU-E) in vitro, which infected by HCMV and/or affected by all-trans retinoic acid (ATRA).
METHODS: Cord blood was collected from fetal placenta umbilical vein of 12 cases. With the colony culture in vitro and the fluorescence quantity reverse transcriptase polymerase chain reaction method, HCMV-AD169 and (or) ATRA (6×10-8 mol/L) was used to intervene the CFU-E colony formation. The expressions of Hoxb2 and Hoxb4 genes on the differentiation progress of hematopoietic stem cells to CFU-E affected by HCMV and/or ATRA were observed in the blank, ATRA, HCMV-AD169, ATRA+HCMV groups at 3, 7 and 10 days. 
RESULTS AND CONCLUSION: The quantity of Hoxb2 and Hoxb4 genes was expressed on day 3, obviously increased on day 7 and highest on day 10 in each group. Compared with the blank group, expressions of Hoxb2 and Hoxb4 genes were down-regulated obviously in the HCMV group. Compared with the HCMV group, expressions of Hoxb2 and Hoxb4 genes were obviously up-regulated in the HCMV+ATRA group (P < 0.05). Results suggest that abnormal expression of Hoxb2 and Hoxb4 gene induced by HCMV may play an important role in abnormal hematogenic damage. Hoxb2 and Hoxb4 gene were correlated to the process of in the proliferation and differentiation of erythroid progenitor cells. ATRA (6×10-8 mol/L) can up-regulate the expression of Hoxb2 and Hoxb4 gene significantly in normal erythroid progenitor cells, and those infected by HCMV.

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