中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7700-7703.doi: 10.3969/j.issn.1673-8225.2010.41.024

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

兔膝骨性关节炎关节液中白细胞介素1β和肿瘤坏死因子α表达与艾灸的影响　

熊  勇1，彭  锐1，夏数数2   

  1. 1湖北中医药大学针灸骨伤学院，湖北省武汉市 430061；2湖北大学校医院骨科，湖北省武汉市 430062
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 夏数数，博士，副主任医师，湖北大学校医院骨科，湖北省武汉市 430062 xss@hubu.edu.cn
  • 作者简介:熊勇☆，男，1978年生，湖北省武汉市人，汉族，博士，讲师，主要从事老年性骨关节病的研究。
  • 基金资助:

    湖北省自然科学基金面上项目（2009CDB111）：温和灸预防兔膝骨性关节炎过程中对软骨细胞凋亡的研究。

Effect of moxibustion on the expressions of interleukin-1 beta and tumor necrosis factor alpha in joint synovial fluid of rabbits with knee osteoarthritis

Xiong Yong1, Peng Rui1, Xia Shu-shu2   

  1. 1 College of Acupuncture-moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan  430061, Hubei Province, China; 2 Department of Orthopaedics, Hospital of Hubei University, Wuhan  430061, Hubei Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Xia Shu-shu, Doctor, Associate chief physician, Department of Orthopaedics, Hospital of Hubei University, Wuhan 430061, Hubei Province, China xss@hubu.edu.cn
  • About author:Xiong Yong☆, Doctor, Lecturer, College of Acupuncture- moxibustion and Orthopaedics, Hubei University of Chinese Medicine, Wuhan 430061, Hubei Province, China xiongyong1978@163.com
  • Supported by:

     the General Program of Hubei Provincial Natural Science Foundation, No. 2009CDB111*

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摘要：

背景：灸法具有操作简便、无痛苦、疗效明显的优点，在临床上治疗膝骨性关节炎有较好的疗效。但目前有关灸疗对膝骨性关节炎的早期防治作用的研究还不够深入。
目的：观察艾灸对兔膝骨性关节炎关节液中白细胞介素1β和肿瘤坏死因子α水平的影响，探讨艾灸防治膝骨性关节炎的可能机制。
方法：采用改良伸直位固定法制备兔膝骨性关节炎模型，造模后立即干预。艾灸组艾灸关元、左后肢足三里、血海、内膝眼、犊鼻和阳陵泉等穴，每穴10 min，1次/d；几丁糖组关节内注射几丁糖，0.5 mL/关节，1次/周。造模8周，采用ELISA法检测各组关节液中白细胞介素1β和肿瘤坏死因子α的水平。
结果与结论：膝骨性关节炎后，兔的Mankin评分明显升高(P < 0.01)，艾灸和几丁糖治疗可降低Mankin评分(P < 0.01)，且两者比较差异无显著性意义(P > 0.05)。ELISA结果显示：模型组关节液中白细胞介素1β和肿瘤坏死因子α的水平明显增高(P < 0.01)，艾灸组和几丁糖组关节液中白细胞介素1β和肿瘤坏死因子α水平均较模型组低(P < 0.01)，艾灸组和几丁糖组间差异无显著性意义(P > 0.05)。提示艾灸可通过降低关节液中白细胞介素1β和肿瘤坏死因子α的水平对兔膝骨性关节炎起防治作用。

关键词: 艾灸, 骨性关节炎, 白细胞介素1β, 肿瘤坏死因子α, 几丁糖

Abstract:

BACKGROUND: Moxibustion has advantages of the simple operation, painlessness and significantly curative effect, there was evidence that moxibustion shows definite clinical effectiveness in treating knee osteoarthritis. However, studies regarding effects of moxibustion on treating knee osteoarthritis remain still not deep enough.
OBJECTIVE: To observe the effect of moxibustion on the concentrations of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in joint synovial fluid in rabbit with experimental knee osteoarthritis, and to explore its potential mechanism.
METHODS: Model of knee osteoarthritis was established with modified with plaster cast in extension position, and intervention was used at the beginning of model preparation. Animals in the moxibustion group were treated with moxibustion applied on Guanyuan (CV 4), Zusanli (ST 36), Xuehai (SP 10), Neixiyan (EX-LE4), Dubi (ST 35) and Yanglingquan (GB 34) (each acupoint for 10 minutes, once a day); animals in the chitosan group were treated with chitosan (20 mg/mL, 0.5 mL per inject, once a week) and in model group with nothing. At 8 weeks after model preparation, concentrations of IL-1β and TNF-α in joint synovial fluid were detected by Enzyme-linked immunosorbent assay (ELISA).
RESULTS AND CONCLUSION: Mankin score of rabbit was increased after knee osteoarthritis (P < 0.01), but the scores in the moxibustion group and chitosan group were lower than in model group respectively (P < 0.01), the scores in moxibustion group were not significantly different from in chitosan group (P > 0.05). The concentrations of IL-1β and TNF-α in the model group were significant increased (P < 0.01). Compared with the model group, the IL-1β and TNF-α in the moxibustion and chitosan groups were lower (P < 0.01), but there was no notable differences between the moxibustion group and chitosan group (P > 0.05). Moxibustion can decrease the joint synovial fluid level of IL-1β and TNF-α, which may contribute the therapeutic effect of moxibustion on knee osteoarthritis.

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