中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7529-7532.doi: 10.3969/j.issn.1673-8225.2010.40.026

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

羟乙基淀粉一步与两步法分离骨髓单个核细胞及血小板的比较

马  艳1，陈 双2，毕晓娟1，江  明1，2   

  1. 新疆医科大学第一附属医院，1医学研究中心所属干细胞研究中心；2血研所，新疆维吾尔自治区乌鲁木齐市  830054
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 通讯作者: 江明，教授，主任医师，硕士生导师，新疆医科大学第一附属医院医学研究中心所属干细胞研究中心、新疆医科大学第一附属医院血研所，新疆维吾尔自治区乌鲁木齐 830054 jiangmingyy@yahoo.com.cn
  • 作者简介:马艳，女，1958年生，新疆维吾尔自治区乌鲁木齐市人，汉族，1985年乌鲁木齐军区军医学校毕业，副主任技师，主要从事干细胞实验研究。 mayan1121@126.com

Mononuclear cells and platelets isolated from human bone marrow using hydroxyethyl starch by one-step way versus two-step way

Ma Yan1, Chen Shuang 2, Bi Xiao-juan 1, Jiang Ming 1, 2   

  1. 1 Stem Cell Laboratory, Medical Research Center, 2 Institute of Hematology, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Jiang Ming, Professor, Master’s supervisor, Chief physician, Stem Cell Laboratory, Medical Research Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Institute of Hematology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jiangmingyy@yahoo.com.cn
  • About author:Ma Yan, Associate chief technician, Stem Cell Laboratory, Medical Research Center, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mayan1121@126.com

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312

被引次数

2

摘要：

背景：羟乙基淀粉和淋巴细胞分离液两步法分离骨髓有核细胞，可以提高单个核细胞的浓缩效率，但对血小板残留量的研究未见报道。
目的：明确羟乙基淀粉两种分离方法浓缩骨髓单个核细胞的效率和残留血小板的量。
方法：骨髓来自在新疆医科大学第一附属医院行成体自体骨髓有核细胞移植治疗相关疾病分离骨髓的检测标本。采用羟乙基淀粉自然沉降和羟乙基淀粉沉降后再用淋巴细胞分离液分离骨髓细胞。观察两种方法分离骨髓细胞后有核细胞、单个核细胞、血小板数。
结果与结论：羟乙基淀粉两步法分离骨髓后有核细胞悬液中单个核细胞均值为89%，一步法为45%。两步法浓缩单个核细胞的百分率高于一步法(P < 0.05)；残留血小板数(中位数：73.00)低于一步法(中位数：367.50)(P < 0.05)。结果表明，羟乙基淀粉和淋巴细胞分离液两步法分离骨髓细胞中单个核细胞和血小板的效果优于一步法。

关键词: 羟乙基淀粉, 淋巴细胞分离液, 分离, 单个核细胞, 血小板

Abstract:

BACKGROUND: It can improve the concentrated efficiency of mononuclear cell to use hydroxyethyl starch with lymphocyte separating medium. However, there is no report addressing the residual volume of platelets.
OBJECTIVE: To detect the efficiency of isolating mononuclear cells and residual volume of platelets using hydroxyethyl starch by one step or two-step way.
METHODS: Bone marrow was obtained from the samples of patients treated with adult autologous bone marrow karyocyte transplantation performed at the First Affiliated Hospital, Xinjiang Medical University, China. Bone marrow tissues acquired from patients were sedimentated by hydroxyethyl starch solution only, or followed by further centrifugation with lymphocyte separating medium. Karyocytes, mononuclear cells and platelet number by these two methods were observed.
RESULTS AND CONCLUSION: The average percentage of mononuclear cells in two-step group (89%) was significantly higher than one-step group (45%) (P < 0.05). Moreover, the number of platelets was lower in two-step group (median: 73.00) than one-step group (median: 367.50) (P < 0.05). Results have suggested that the isolation efficiency of using hydroxyethyl starch solution by two-step way from human bone marrow was higher than one-step way.

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