关键词: 血液透析器, 中空纤维, 体外评价, 方法, 血液相容性

Abstract:

BACKGROUND: The hollow fiber in hemodialyzer has a large-scale and long-time contact with the blood of the renal dialysis patients. The evaluation of the hollow fiber’s hemocompatibility is very important.
OBJECTIVE: The evaluation method of in vitro hemocompatibility of the hemodialyzer was primarily established by twice evaluating hollow fiber’s hemocompatibility in a hemodialyzer.
METHODS: The hollow fiber of the sample hemodialyzer was cut into 3-cm length, and 100 hollow fibers were put into the silicified glass tube as a sample. In this experiment, the sample hemodialyzer was tested twice with two different commercially available hemodialyzers as controls. The similar surface-area hollow fiber of the control hemodialyzers was disposed in the same way. 1.2 mL fresh human blood was added into every silicified glass tube, then all tubes was put onto the slightly sloping rotary incubator at 37 ℃ and rotated at the speed of 30 r/min for 30 minutes. Finally, the blood in all silicified glass tubes was detected for blood cell analysis, coagulation analysis and serum total complements activity determination respectively.
RESULTS AND CONCLUSION: Different parameters exhibited significant differences between the sample hollow fiber and two control hollow fibers. But the remarkable consume of fibrinogen and platelet was similar in the two tests, and the “|(parameter of sample-parameter of control)|/parameter of control ×100%” was far greater than 15%.So the difference on the hemocompatibility between the sample hollow fiber and control hollow fibers is not acceptable, which indicates that the in vitro hemocompatibility evaluation method has a good reproducibility.