中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6938-6942.doi: 10.3969/j.issn.1673-8225.2010.37.023

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

丹参素在大鼠主要组织分布中的动力学特征

周陆怡1，鲁澄宇2   

  1. 广东医学院，1实验管理处，2药学院药理学教研室，广东省东莞市  523808
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 作者简介:周陆怡★，女，1982年生，重庆市人，汉族，硕士，主要从事药物代谢动力学研究。 zly820601@163.com
  • 基金资助:

    广东省自然基金，标号为KZ0701，名称：机遇三维体素数据库的骨组织计量学分析及生物力学模拟。

Pharmacokinetic features of Danshensu in rat main tissues

Zhou Lu-yi1, Lu Cheng-yu2   

  1. 1 Department of Laboratory Management, 2 Department of Pharmacology, College of Pharmacy, Guangdong Medical College, Dongguan  523808, Guangdong Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Zhou Lu-yi★, Master, Department of Laboratory Management, Guangdong Medical College, Dongguan 523808, Guangdong Province, China zly820601@163.com
  • Supported by:

     the Natural Science Foundation of Guangdong Province, No. KZ0701*

PDF

366

被引次数

4

摘要：

背景：近年来对丹参素体内药动学的研究已有很多，但丹参素在大鼠主要组织中特别是皮肤和骨组织中的分布动力学研究尚未见全面的报道。
目的：研究大鼠静脉注射复方丹参液后丹参素在主要组织中的分布动力学。
方法：采用液液萃取法对SD大鼠血清和主要组织样品(肾、脾、心、肝、肺、腹部皮肤、背部皮肤和骨组织)，以对羟基苯甲酸为内标，甲醇-1%冰醋酸(8∶92)为流动相，用高效液相色谱法于280 nm处检测，液质联用进行定性分析。
结果与结论：大鼠静脉注射复方丹参液后，除脑组织外，在血清和其它组织中均检测到丹参素，用所建立的方法绘制了其经时血药浓度曲线和组织浓度柱形图。研究表明大鼠静脉注射复方丹参液后，可以在主要组织中(除脑外)快速分布，其动力学类型属二室模型。

关键词: 丹参素, 血药浓度, 组织分布, 药代动力学, 高效液相色谱, 质谱

Abstract:

BACKGROUND: The in vivo pharmacokinetic features of Danshensu have been reported, but there are few studies concerning the distribution of Danshensu in rat main tissues.
OBJECTIVE: To study the distribution and pharmacokinetics of Danshensu in rat main tissues following intravenous injection of compound Danshen solution.
METHODS: Liquid-liquid extraction was used to deal with the serum and main tissues (kidney, spleen, heart, liver, lung, abdomen and back skins, as well as bone tissues). The conditions for HPLC were: 4-hydroxy-benzoic acid served as the internal standard, the CH3OH-1%CH3COOH (8:92) served as mobile phase, and detection wavelength was 280 nm. 
RESULTS AND CONCLUSION: Except the brains, Danshensu could find in the rat serum and the main tissues after intravenous injection of compound Danshen solution. The column of serum concentration-time and tissues concentration-time was drew using the established methods. The results demonstrated that except the brains, Danshensu could distribute rapidly in main tissues after intravenous injection of compound Danshen solution, and the pharmacokinetics fitted to two-compartment model.

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