中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (29): 5346-5350.doi: 10.3969/j.issn.1673-8225.2010.29.007

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

羟基磷灰石表面修饰人工角膜钛支架的生物相容性：电镜观察

李朝辉1，黄一飞1，马  骁1，黄靖香2，崔福斋3   

  1. 解放军总医院，1眼科，2骨科研究所，北京市  100853；3清华大学材料科学工程系，北京市  100084
  • 出版日期:2010-07-16 发布日期:2010-07-16
  • 通讯作者: 黄一飞，教授，博士，博士生导师，解放军总医院眼科，北京市 100853
  • 作者简介:李朝辉★，男，1965年生，山东省蓬莱市人，1999年解放军总医院毕业，硕士，副主任医师，主要从事眼前节的基础和临床工作。 zhaohuili650@hotmail.com
  • 基金资助:

    中国人民解放军十五课题(01MA109)，课题名称：一体式人工角膜研制及实验研究；中国人民解放军十一五课题(06MA281)，课题名称：复合人工角膜研制及应用研究。

Biocompatibility of hydroxyapatite surface modified titanium skirt for keratoprosthesis: Scanning electron microscopy observation

Li Zhao-hui1, Huang Yi-fei1, Ma Xiao1, Huang Jing-xiang2, Cui Fu-zhai3   

  1. 1 Department of Ophthalmology, General Hospital of China PLA, Beijing   100853, China; 2 Orthopaedics Institute, General Hospital of China PLA, Beijing   100853, China; 3 Department of Materials Science and Engineering, Tsinghua University, Beijing   100084, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Huang Yi-fei, Professor, Doctor, Doctoral supervisor, Department of Ophthalmology, General Hospital of China PLA, Beijing 100853, China
  • About author:Li Zhao-hui★, Master, Associate chief physician, Department of Ophthalmology, General Hospital of China PLA, Beijing 100853, China zhaohuili650@hotmail.com
  • Supported by:

     Project of Chinese PLA During the 10th Five-Year Plan Period, No. 01MA109*, 06MA281*

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摘要：

背景：人工角膜是双眼角膜盲患者复明的希望，提高人工角膜材料的生物相容性使人工角膜与宿主角膜达到生物愈合是目前人工角膜的研究方向。
目的：扫描电子显微镜观察经羟基磷灰石表面修饰能否增加人工角膜纯钛支架的生物相容性。
方法：采用酸碱两步预处理法在人工角膜钛支架表面快速沉积羟基磷灰石涂层。将第4~6代兔角膜基质成纤维细胞直接接种于羟基磷灰石修饰的钛支架、纯钛支架及玻璃表面，3，24，48，72 h后，扫描电子显微镜观察材料表面的细胞黏附，伸展及增殖情况；将18只正常新西兰白兔随机分为2组，于右眼角膜基质层内分别植入羟基磷灰石修饰的钛支架、纯钛支架，术后6，12周取出人工角膜支架，扫描电子显微镜观察材料表面角膜组织贴附生长状态。
结果与结论：体外实验显示，细胞接种3 h和24 h后，细胞扩展面积及细胞张力丝长度：羟基磷灰石修饰的钛支架>玻璃>纯钛表面，羟基磷灰石修饰的钛支架表面的活细胞数多于其他材料表面(P < 0.05)。72 h后，羟基磷灰石修饰的钛支架表面完全被胶原覆盖。体内实验显示，扫描电子显微镜观察羟基磷灰石修饰的钛支架表面细胞外基质生长良好，与羟基磷灰石贴附紧密。而纯钛支架仅为角膜组织简单包裹。说明人工角膜纯钛支架经羟基磷灰石表面修饰后，其生物相容性增加。

关键词: 人工角膜, 羟基磷灰石, 钛, 表面改性, 生物相容性, 扫描电子显微镜

Abstract:

BACKGROUND: Keratoprosthesis surgery may be alternative in patients with corneal blindness of both eyes. Currently, researches for improving biocompatibility between the keratoprosthesis and the host tissue to achieve biological healing are predominant.
OBJECTIVE: To evaluate the biocompatibility of hydroxyapatite surface modified titanium skirt for keratoprosthesis by scanning electron microscopy.
METHODS: The bioactive hydroxyapatite coating was prepared on pure titanium for keratoprosthesis by an acid-alkali chemical pretreatment. The fibroblasts of rabbit cornea at 4 to 6 passages were directly seeded on hydroxyapatite modified titanium surfaces, pure titanium surfaces and glass surfaces. Cell adhesion, proliferation and morphology were detected at 3, 24, 48, 72 hours by scanning electron microscopy. A total of 18 normal New Zealand white rabbits were randomly divided into two groups. Skirt of hydroxyapatite modified titanium and pure titanium were implanted into the right eye corneal stroma of rabbits for 6 and 12 weeks, respectively. The growth and adhesion of the corneal tissue on the surface of skirt were examined by scanning electron microscopy.
RESULTS AND CONCLUSION: The in vitro experiment showed that, cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers than that on the pure titanium at 3 and 24 hours. Both hydroxyapatite modified titanium and pure titanium were superior to glass surface. The number of viable cells was the highest on hydroxyapatite modified titanium surfaces (P < 0.05). At 72 hours, hydroxyapatite modified titanium surfaces were totally covered by collagen. The in vivo experiment showed that, the extracellular matrix deposited well on the surface of hydroxyapatite modified titanium, tightly adhered with hydroxyapatite. The pure titanium was only covered with corneal tissues. Hydroxyapatite surface modification could improve the biocompatibility of pure titanium skirt for keratoprosthesis.

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