中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 4951-4954.doi: 10.3969/j.issn.1673-8225.2010.27.003

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

兔椎间盘移植人骨髓间充质干细胞的实验观察

王月秋，陈伯华   

  1. 青岛大学医学院附属医院脊柱外科，山东省青岛市     266003
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 陈伯华，教授，博士生导师，青岛大学医学院附属医院脊柱外科，山东省青市266003
  • 作者简介:王月秋，男，1975年生，山东省济宁市人，汉族，青岛大学医学院在读硕士，主治医师，主要从事脊柱外科方面的研究。 qingdayanjiusheng@163.com
  • 基金资助:

    青岛市科技发展计划项目(07-2-1-12-nsh-1)

Experimental observation of rabbit intervertebral disc transplantation with human bone marrow mesenchymal stem cells

Wang Yue-qiu, Chen Bo-hua   

  1. Department of Spine Surgery, Affiliated Hospital of Medical College of Qingdao University, Qingdao  266003, Shandong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Chen Bo-hua, Professor, Doctoral supervisor, Department of Spine Surgery, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
  • About author:Wang Yue-qiu, Studying for master’s degree, Attending physician, Department of Spine Surgery, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China qingdayanjiusheng@163.com
  • Supported by:

    the Science and Technology Development Program of Qingdao City, No. 07-2-1-12-nsh-1*

PDF

377

被引次数

7

摘要：

背景：国内外动物实验多是研究同种异体骨髓间充质干细胞的移植，但对人骨髓间充质干细胞在活体椎间盘内的存活情况还知之甚少。

目的：观察人骨髓间充质干细胞在兔椎间盘内的存活情况。

方法：选取新西兰大白兔15只，每只兔子的椎间盘被分为3组，即空白组(L1~2)、生理盐水组(L2~3)、绿色荧光蛋白标记的人骨髓间充质干细胞移植组(L3~4，L4~5，L5~6)。空白组不注射，生理盐水组髓核内注射生理盐水25 μL，绿色荧光蛋白标记的人骨髓间充质干细胞移植组注射1×109 L-1绿色荧光蛋白标记的人骨髓间充质干细胞生理盐水混悬液25 μL。移植后1，2，4，6，8周取材，荧光显微镜观察荧光细胞的分布情况及其数量。

结果与结论：绿色荧光蛋白标记的人骨髓间充质干细胞移植组移植后1，2，4，6，8周标本切片，髓核内均可见绿色荧光细胞。移植后6，8周的荧光细胞数量明显少于移植后1，2，4周(P < 0.001)。提示移植后8周以内，人骨髓间充质干细胞可在兔椎间盘内存活。

关键词: 骨髓间充质干细胞, 细胞移植, 椎间盘, 异种移植, 存活, 兔

Abstract:

BACKGROUND: Numerous studies have demonstrated allogenic bone marrow mesenchymal stem cells (BMSCs) transplantation, but few reports have addressed the survival of human BMSCs in the intervertebral discs in vivo.

OBJECTIVE: To observe the survival of human BMSCs in the rabbit intervertebral discs.

METHODS: A total of 15 New Zealand white rabbits were selected, and each of the rabbit intervertebral disc was divided into 3 groups, blank group(L1-2), physiological saline (PS) group(L2-3), green fluorescent protein (GFP) transfection of human BMSCs transplantation group(L3-4, L4-5, L5-6). The samples in the blank group were not injected; those in the PS group were injected with 25 μL PS into nucleus pulposus; those in the human BMSCs/GFP transplantation group were injected with 25 μL human BMSCs/GFP-PS suspension(1×109/L) into nucleus pulposus. At 1, 2, 4, 6, 8 weeks after operation, the specimen was taken out. Fluorescence microscope was used to observe distributed situation and quantity. 

RESULTS AND CONCLUSION: Samples were sliced into sections at 1, 2, 4, 6, 8 weeks following GFP-labeled human BMSCs transplantation. Green fluorescent cells were seen in the nucleus pulposus. The number of fluorescent cells was significantly less at 6 and 8 weeks than at 1, 2, 4 weeks following transplantation(P < 0.001). Results have suggested that within 8 weeks, the human BMSCs could be survived in the rabbit intervertebral disc.

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