中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4285-4289.doi: 10.3969/j.issn.1673-8225.2010.23.024

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

养心通脉有效部位方与急性心肌梗死大鼠骨髓间充质干细胞的动员及定向归巢

李勇华1，2，袁肇凯2，郑景辉2，王丽萍2，简维雄2，黄献平2   

  1. 1重庆三峡医药高等专科学校中医系，重庆市 404120；
    2湖南中医药大学中医诊断研究所，湖南省长沙市  410007
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 李勇华，男，1976年生，湖南省湘潭市人，汉族，湖南中医药大学在读博士，主治医师，主要从事中医心病证治方面的研究。 liyonghua200888@sina.com
  • 作者简介:李勇华，男，1976年生，湖南省湘潭市人，汉族，湖南中医药大学在读博士，主治医师，主要从事中医心病证治方面的研究。 liyonghua200888@sina.com
  • 基金资助:

    国家自然科学基金资助项目(30772696)，课题名称“养心通脉有效部位方对大鼠缺血心肌骨髓间充质干细胞移植的影响”；
    国家自然科学基金资助项目(30973717)，课题名称“冠心病血瘀证的遗传特征研究”；
    2009年湖南省研究生创新基金(CX2009B168)，课题名称“心血瘀阻证微环境对骨髓间充质干细胞向心肌样细胞分化的影响”。

Effects of active principle region of Yangxing Tongmai Formula on bone marrow mesenchymal stem cell mobilization and homing in rats with acute myocardial infarction

Li Yong-hua1, 2, Yuan Zhao-kai2, Zheng Jing-hui2, Wang Li-ping2, Jian Wei-xiong2, Huang Xian-ping2   

  1. 1Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing   404120, China;
    2Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha   410007, Hunan Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yuan Zhao-kai, Master, Professor, Doctoral supervisor, Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China yuanzk1520@yahoo.com.cn
  • About author:Li Yong-hua, Studying for doctorate, Attending physician, Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China; Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China liyonghua200888@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772696*, 30973717*;
    the Postgraduate Innovation Foundation of Hunan Province in 2009, No. CX2009B168*

PDF

390

被引次数

24

摘要：

背景：急性梗死心肌修复过程中，寻找可有效提高外周血骨髓间充质干细胞的数量、并动员其定向归巢于损伤心肌的骨髓间充质干细胞动员剂尤为关键。

目的：探讨养心通脉有效部位方对急性心肌梗死大鼠骨髓间充质干细胞动员入血及定向归巢于梗死心肌的影响。

方法：养心通脉有效部位方主要成分包括人参皂苷、丹参酮ⅡA、总生物碱、人参多糖等，由中南大学药学院依课题组稳定的制作工艺制作提供。SD大鼠70只，取8只大鼠作为正常对照组，剩余62只大鼠建立急性心肌梗死模型。取造模成功的32只大鼠，随机分为养心通脉有效部位方组、复方丹参注射液组、重组人粒细胞集落刺激因子组、模型对照组，各组均于造模3 h后给与对应药物，连续5 d。流式细胞仪检测外周血CD34+细胞数，免疫组化染色检测梗死心肌边缘区CD34+细胞数。

结果与结论：与模型对照组比较，重组人粒细胞集落刺激因子组、养心通脉有效部位方组外周血CD34+细胞数均明显增加(P < 0.01)；心肌梗死边缘区胞浆CD34+细胞数均显著增加(P < 0.01)。与重组人粒细胞集落刺激因子组比较，养心通脉有效部位方组心肌梗死边缘区胞浆CD34+细胞数显著增加(P < 0.01)。养心通脉有效部位方能促进骨髓干细胞动员入血，并定向归巢于梗死心肌，其作用与重组人粒细胞集落刺激因子大体相当，在归巢CD34+细胞方面甚至强于重组人粒细胞集落刺激因子，是一种良好的骨髓间充质干细胞动员剂。

关键词: 动员, 归巢, 重组人粒细胞集落刺激因子, 中药, 养心通脉有效部位方, 骨髓间充质干细胞

Abstract:

BACKGROUND: During acute infarct myocardium repair process, it is important to seek mobilization agent that can elevate number of peripheral blood bone marrow mesenchymal stem cells (BMSCs) and induce BMSCs homing to damaged myocardium.

OBJECTIVE: To explore the effect of active principle region of Yangxin Tongmai Formula (apr-YTF) on the BMSC mobilization and homing of infarct myocardium in rats.

METHODS: The apr-YTF was primarily composed of ginsenoside, tanshinone ⅡA, total alkaloids, and panaxan, which was supplied by College of Pharmacy, Central South University in accordance with the technique of this group. Among 70 Sprague Dawley rats, 8 rats were considered as normal control group, and the remaining 62 rats was used to establish models of acute myocardial infarction. A total of 32 rat models were randomly assigned to apr-YTF group, Danshen injection group, recombinant human granulocyte colony stimulating factor (rhG-CSF) group and model control group. After 3-hour modeling, corresponding drugs were given in each group for 5 days. Peripheral blood CD34+ cell number was determined using flow cytometry. CD34+ cell number was detected at marginal zone surrounding the infarct myocardium utilizing immunohistochemical staining.

RESULTS AND CONCLUSION: Number of peripheral blood CD34+ cells increased significantly in the rhG-CSF group and apr-YTF group compared with model control group (P < 0.05). CD34+ cell number at marginal zone surrounding the infarct myocardium increased significantly (P < 0.01). Compared with the rhG-CSF group, CD34+ cell number at marginal zone surrounding the infarct myocardium increased significantly in the apr-YTF group (P < 0.01). The apr-YTF could promote BMSCs mobilization into blood, and directly homing to infarct myocardium. Its effects were identical to rhG-CSF. The effects on homing of CD34+ cells were better than rhG-CSF. It was a good agent for BMSCs mobilization.

中图分类号: