中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4207-4210.doi: 10.3969/j.issn.1673-8225.2010.23.007

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

第4代大鼠脂肪间充质干细胞的免疫表型鉴定

李东飞，杨  春，李  桢，戴景兴，原  林   

  1. 南方医科大学人体解剖学教研室 广东省广州市 510515
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 原 林，教授，南方医科大学人体解剖学教研室，广东省广州市 510515 yuanl@fimmu. com
  • 作者简介:李东飞，男，1972年生，河南省孟州市人，汉族，南方医科大学在读博士，主治医师，主要从事间充质干细胞的基础与应用研究。 mzlidongfei@ 126.com
  • 基金资助:

    国家重点基础研究计划(973计划)项目（2007CB512705）
    国家自然科学基金青年科学基金项目（30801464）

Immunophenotype determination of rat adipose tissue-derived mesenchymal stem cells at passage 4

Li Dong-fei, Yang Chun, Li Zhen, Dai Jing-xing, Yuan Lin   

  1. Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Yuan Lin, Professor, Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China yuanl@fimmu.com
  • About author:Li Dong-fei, Studying for doctorate, Attending physician, Department of Human Anatomy, South Medical University, Guangzhou 510515, Guangdong Province, China mzlidongfei@126. com
  • Supported by:

    the National Basic Research Program (973 Program) of China, No.2007CB512705*;
    the Youth Science Program of National Natural Science Foundation of China, No.30801464*

PDF

408

被引次数

12

摘要：

背景：有文献证实以酶消化法可获得在脂肪组织间充质干细胞，传至第10代时细胞生长仍良好，但表面标志物表达下降。
目的：分离培养大鼠脂肪组织间充质干细胞，传代至第4代，检测免疫表型。
方法：从6只SD大鼠腹股沟脂肪垫中分离培养脂肪组织间充质干细胞，以贴壁细胞扩增传代至第4代。相差显微镜下观察脂肪组织间充质干细胞的形态，并用流式细胞术鉴定脂肪组织间充质干细胞的表面标志物CD11b，CD29，CD45，CD49d，CD90 和 CD106的表达率。
结果与结论：大鼠脂肪组织间充质干细胞呈现类似成纤维细胞样形态学特征，免疫表型分析显示CD29和CD90 呈阳性，而CD11b、CD45、CD49d和CD106呈阴性反应。结果提示通过这种酶消化法获得的SD大鼠的脂肪组织间充质干细胞，免疫表型分析显示符合间充质干细胞的特征。

关键词: 脂肪组织, 脂肪间充质干细胞, 免疫表型, 大鼠, 细胞鉴定

Abstract:

BACKGROUND: Many studies have confirmed that adipose tissue-derived mesenchymal stem cells (ADMSCs) can be collected using enzyme digestion method. At passage 10, ADMSCs grew well, but their surface marker expression decreases.
OBJECTIVE: To isolate and cultivate rat ADMSCs and detect immunophenotype at passage 4.
METHODS: ADMSCs of six Sprague-Dawley rats from inguinal fat pads were isolated and cultured. Adherent cells were amplified till passage 4. Morphology of ADMSCs was observed under a phase contrast microscope. The immunophenotype markers including CD11b, CD29, CD45, CD49d, CD90 and CD106 of ADMSCs were determined using flow cytometry. 
RESULTS AND CONCLUSION: Rat ADMSCs showed fibroblastic-like morphologic characteristics. The immunophenotype analysis revealed that the rat ADMSCs were positive for CD29 and CD90, but negative for CD11b, CD45, CD49d and CD106. The rat ADMSCs can be obtained through enzyme-based isolation procedures. The results revealed that the immunophenotype characterization of rat ADMSCs is consistent with mesenchymal stem cells.

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