中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 1967-1970.doi: 10.3969/j.issn.1673-8225.2010.11.017

• 组织构建与生物力学 tissue construction and biomechanics • 上一篇    下一篇

氟化物对幼年大鼠股骨生物力学参数的影响

吴天秀1，廖进民2，陈 艳2，黄连芳2，陈文双2   

  1. 1 广东医学院人体解剖学教研室，广东省湛江市  524023；
    2广东省天然药物开发研究重点实验室，广东省湛江市 524023
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 廖进民，博士，教授，硕士导师，广东省天然药物开发研究重点实验室，广东省湛江市 524023 jinminliao@yahoo.com.cn
  • 作者简介:吴天秀，女，1978年生，广东省茂名市人，汉族，2008年广东医学院毕业，硕士，讲师，主要从事骨生物学研究。 wutianxiu2005@163.com
  • 基金资助:

    广东省自然科学基金资助项目06301445

Effects of fluorid on biomechanical parameters of the femur in young rats

Wu Tian-xiu1, Liao Jin-min2, Chen Yan2, Huang Lian-fang2, Chen Wen-shuang2   

  1. 1Department of Human Anatomy, Guangdong Medical College, Zhanjiang  524023, Guangdong Province, China;
    2Guangdong Key Laboratory for Research and Development of Natural Drugs,  Zhanjiang  524023, Guangdong Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Liao Jin-min, Doctor, Professor, Master’s supervisor, Guangdong Key Laboratory for Research and Development of Natural Drugs, Zhanjiang 524023, Guangdong Province, China jinminliao@yahoo.com.cn
  • About author:Wu Tian-xiu, Master, Lecturer, Department of Human Anatomy, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China wutianxiu2005@163.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 06301445*

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495

被引次数

2

摘要：

背景：氟化物治疗骨质疏松一直备受争议，其对幼年大鼠的股骨生物力学参数影响的文献报道较少。

目的：观察氟化钠对幼年大鼠股骨骨生物力学参数的影响。

方法：SPF级的2月龄SD大鼠共90只，雌雄各半，被随机分成9组：对照组(幼年组、成年组、长期组)和用药组(幼年高氟组、幼年低氟组、成年高氟组、成年低氟组、幼年长期高氟组、幼年长期低氟组)。对照组灌胃生理盐水，用药组分别按相应时间给予不同剂量的氟化钠灌胃。实验结束后，大鼠被异戊巴比妥钠麻醉后，心脏取血处死大鼠。取大鼠左侧股骨进行三点弯曲力学试验，观察氟化物对幼年大鼠股骨最大载荷和刚度的影响。

结果与结论：与幼年对照组相比，幼年高氟组股骨的最大载荷和刚度分别下降13.18%和13.61%(P < 0.05)，幼年低氟组的两项指标变化差异无显著性意义。与长期高氟组相比，幼年高氟组的最大载荷和刚度分别下降17.22%和17.17%(P < 0.05)；长期低氟组的最大载荷和刚度分别增加了18.33%和19.15%(P < 0.05)；成年高氟组和成年低氟组的最大载荷和刚度也有明显增加(P < 0.05)。提示幼年大鼠对高剂量的氟化物更为敏感，高剂量的氟化物可降低大鼠的骨质量，随着氟化钠使用时间的延长，逐渐表现出对生长期大鼠骨质量的负作用。

关键词: 氟化钠幼年大鼠, 骨生物力学股骨, 骨组织工程

Abstract:

BACKGROUND: Fluoride treatment of osteoporosis has been controversial. Literatures addressing the effect of fluoride on bone bio-mechanical parameters of femur in young rats are few.

OBJECTIVE: To study the effects of fluoride on bone biomechanical parameters of femur in young rats.

METHODS: Ninety 2-month-old SPF Sprague Dawley rats, half male and female, were randomly divided into 9 groups: control group (young, adult and long-time) and drug-administered group (young high-fluoride, young low-fluoride, adult high-fluoride, adult low-fluoride, long-term high-fluoride and long-term low-fluoride). Rats in the control group were orally administered with physiological saline, while in the drug-administered group were given orally with different dose fluoride at the corresponding times. After experiment, rats were sacrificed under anaesthesia. Three-point bending test was performed at the left femur. The effects of fluoride on maximum load and rigidity of femur were measured.

RESULTS AND CONCLUSION: Compared with young control group, the maximum load and the rigidity of femur in the young high-fluoride group were decreased by 13.18% and 13.61%, respectively (P < 0.05), which had no dramatically difference in the young low-fluoride group. Compared with long-term high-fluoride group, the maximum load and the rigidity of femur in the young high-fluoride were decreased by 17.22% and 17.17% (P < 0.05), which were obvious increased in the long-term low-fluoride group by 18.33% and 19.15%, respectively (P < 0.05). The maximum load and the rigidity of femur were strengthened in the adult high-fluoride and adult low-fluoride groups (P < 0.05). The results suggested that young rats are more sensitive to high-dose fluoride, which can reduce bone quality in rats. The negative effects on bone quality of rats were gradually displayed as the prolongation of the period of fluoride.

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