中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (10): 1716-1720.doi: 10.3969/j.issn.1673-8225.2010.10.002

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

碘对骨髓间充质干细胞分化为神经元样细胞存活时间的影响

阎文柱，秦书俭，刘学政，李德华   

  1. 辽宁医学院解剖学教研室，辽宁省锦州市  121001
  • 出版日期:2010-03-05 发布日期:2010-03-05
  • 作者简介:阎文柱，男，1964年生，辽宁省朝阳市人，汉族，2003年东北财经大学毕业，硕士，副教授，主要从事神经生物学研究。 1078607311@ qq.com
  • 基金资助:

    辽宁省教育厅基金资助项目(2006T063，2008422)

Iodine effect on survival time of neuron-like cells differentiated from bone marrow mesenchymal stem cells

Yan Wen-zhu, Qin Shu-jian, Liu Xue-zheng, Li De-hua   

  1. Department of Anatomy, Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Online:2010-03-05 Published:2010-03-05
  • About author:Yan Wen-zhu, Master, Associate professor, Department of Anatomy, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China 1078607311@qq. com
  • Supported by:

    the Foundation of Department of Education of Liaoning Province, No. 2006T063, 2008422*

PDF

393

被引次数

5

摘要：

背景：体外实验中人们发现，常规化学诱导剂诱导骨髓间充质干细胞分化的神经元样细胞存活时间较短，这就限制了其进一步的应用。关于碘是否可以延长诱导的神经元样细胞的存活时间尚未见系统报道。

目的：观察微量元素碘对由骨髓间充质干细胞分化成的神经元样细胞存活时间的影响。

方法：无菌条件下取大鼠第3代间充质干细胞，分为A~F组：A组不加碘离子，B~F组中分别加入质量浓度为2，55，90，125和2 500 mg/L的碘离子，另设空白对照组，同时加化学诱导剂二甲基亚砜向神经元方向诱导分化。对诱导后的细胞进行免疫组织化学检测，观察诱导的神经元样细胞在不同质量浓度碘离子下的存活时间。

结果与结论：加入的碘离子质量浓度在55~125 mg/L时，细胞存活时间可达到5 d，镜下细胞结构完整，5 d后随培养时间的延长，死亡细胞逐渐增多，至1周左右，神经元样细胞几乎全部死亡。碘离子质量浓度为2 mg/L和2 500 mg/L时，细胞存活时间为12~36 h，和未加碘离子组差异无显著性意义，至两三天，神经元样细胞几乎全部死亡。提示适当浓度的碘离子加入到常规化学诱导剂中，可延长骨髓间充质干细胞分化的神经元样细胞的存活时间；加入到化学诱导剂中的碘离子质量浓度过低或过高时，不改变骨髓间充质干细胞分化的神经元样细胞的存活时间。

关键词: 碘, 骨髓间充质干细胞, 分化, 神经元样细胞, 存活时间

Abstract:

BACKGROUND: In vitro experiment has shown that the survival time of conventional chemical induction-induced neuron-like cells differentiated from bone marrow mesenchymal stem cells (BMSCs) was short, which limited its further application. With regard to the possibility of extension of iodine-induced neuron-like cells, the survival time has not yet been professionally reported.

OBJECTIVE: To research the effects of the micro-element iodine on the survival time of neuron-like cells differentiated by BMSCs.

METHODS: Rat mesenchymal stem cells at passage 3 were obtained under sterile condition, and divided into groups A-F. In group A, iodine ion was not added. In groups B-F, iodine ion at mass concentrations of 2, 55, 90, 125 and 2 500 mg/L was added respectively. An additional blank control group was established, and simultaneously the cells were induced into neuron-like cells with dimethyl sulphoxide (DMSO). Cells following induction were subjected to immunohistochemistry. Survival time of neuron-like cells was observed under different mass concentrations of iodine ion.

RESULTS AND CONCLUSION: When mass concentrations of iodine ion were between 55 - 125 mg/L, the survival time of neuron-like cells prolonged to about 5 days and structures of induced cells were intact. From then on, the number of dead cells was gradually increased till approximately one week, all neuron-like cells died. When mass concentrations of iodine ion were 2 mg/L and 2 500 mg/L, cell survival time was from 12-36 hours. No significant difference was determined compared with group A. Till 2 or 3 days, all neuron-like cells died. Above-described results indicated that an appropriated concentration of iodine iron added in the common chemical induction may be benefit for the survival time of the neuron-like cells differentiated by BMSCs, but the effect may be negligible for the survival time of neuron-like cells induced when the added concentration of iodine iron is too low or too high.

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