中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1257-1261.doi: 10.3969/j.issn.1673-8225.2010.07.027

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

黄芪调控体外培养大鼠成骨细胞Ⅰ型胶原蛋白的表达

郭海玲1，王  翔2，徐  宇2，詹红生2，赵咏芳2   

  1. 1上海中医药大学，上海市 201203； 2上海市中医药研究院骨伤科研究所，上海市  201203
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 通讯作者: 赵咏芳，博士，副教授，上海市中医药研究院骨伤科研究所，上海市 201203 zhao_dingding@126.com
  • 作者简介:郭海玲，女，1983年生，山西省浑源县人，汉族，2007年山东中医药大学毕业，主要从事中医骨伤科学研究。 Ghl22007190@163.com
  • 基金资助:

    课题受“中医骨伤科学”国家和上海市重点学科建设项目(100508、T0303)；国家自然基金项目(30371793、30500674)；上海市科委项目(08ZR1419400、08411950300)；上海市青年科技启明星计划(04qmx1434)；上海市教委创新项目(08YZ60)；上海申康医院发展中心课题(SHDC12007312)资助。

Astragalus root injection regulates type Ⅰ collagen expression of rat osteoblasts in vitro

Guo Hai-ling1, Wang Xiang2, Xu Yu2, Zhan Hong-sheng2, Zhao Yong-fang2   

  1. 1 Shanghai University of Traditional Chinese Medicine, Shanghai   201203, China; 2 Institute of Orthopedics and Traumatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai   201203, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Zhao Yong-fang, Doctor, Associate professor, Institute of Orthopedics and Traumatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China zhao_dingding@126.com
  • About author:Guo Hai-ling, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Ghl22007190@163.com
  • Supported by:

    the National and Shanghai Key Program of Traditional Chinese Medical Science of Orthopedics and Traumatology, No. 100508*, T0303*; the National Natural Science Foundation of China, No. 30371793*, 30500674*; the grant from Shanghai Science and Technology Commission, No. 08ZR1419400*, 08411950300*; Shanghai Rising-Star Program, No. 04qmx1434*; the Innovation Program of Shanghai Education Commission, No. 08YZ60*; a grant from Shanghai Shenkang Hospital Development Center, No. SHDC12007312*

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811

被引次数

75

摘要：

背景： Ⅰ型胶原是体外培养成骨细胞所分泌的特异性胶原蛋白，所形成的网状结构也是成骨细胞矿化功能实现的前提，也是反映成骨细胞骨形成能力的重要指标。诸多报道反映了应用单味黄芪促进成骨细胞增殖的功能，但缺少有关黄芪促进Ⅰ型胶原分泌及表达的研究。
目的：观察黄芪注射液对体外培养新生大鼠颅盖骨成骨细胞增殖及分泌Ⅰ型胶原能力的影响。
方法：体外分离培养大鼠成骨细胞。分为用含小牛血清的MEM培养液培养的对照组，分别加入相应体积分数黄芪注射液培养的黄芪组，于培养1，3，5，7，9 d 以四甲基偶氮唑盐法观察成骨细胞增殖的影响；以Real-time PCR方法检测黄芪注射液干预6 h后成骨细胞Ⅰ型胶原蛋白α1 mRNA的表达情况，并用in cell western方法测定培养6 d时成骨细胞Ⅰ型胶原蛋白的分泌。
结果与结论：从培养3 d开始直至培养9 d，与对照组比较，各体积分数的黄芪注射液均能促进成骨细胞的增殖 (P < 0.05)，这一趋势在培养7 d时达到最高峰。不同体积分数的黄芪注射液均能促进COLLα1 mRNA的表达，尤其以15%的黄芪作用明显。以15%和10%的黄芪注射液培养的成骨细胞Ⅰ型胶原的表达量明显高于对照组(P < 0.05)。结果证实，黄芪可以促进体外培养的成骨细胞的增殖和分泌Ⅰ型胶原的能力，但具有一定的量效趋势。

关键词: 黄芪注射液, 成骨细胞, 骨形成, Ⅰ型胶原, 骨组织工程

Abstract:

BACKGROUND: Type Ⅰ collagen is a specific collagen secreted by in vitro cultured osteoblast, and the formed network is the basis of bone mineralization, which also reflects the ability of osteoblast bone formation. Studies have shown astragalus root increased osteoblast proliferation. However, the effect of astragalus root on improving type Ⅰ collagen expression of osteoblast remains poorly understood. 
OBJECTIVE: To evaluate the effect of astragalus root injection on the abilities of rat cranium-derived osteoblast proliferation and type Ⅰ collagen expression.
METHODS: Rat osteoblast was cultured in vitro and divided into control group (MEM culture solution containing calf serum) and astragalus root groups (different concentrations). The effect on osteoblast proliferation was evaluated on days 1, 3, 5, 7, and 9 by MTT method. Moreover, the expression of type Ⅰ collagen protein was observed after 6 hours of treatment with astragalus root injection using in cell western-blot method. In addition, the gene expression of COLLα1 was investigated by real-time PCR method.
RESULTS AND CONCLUSION: From days 3 to 9, the different concentrations of astragalus root injection improved osteoblast proliferation, respectively compared with control group (P < 0.05), and this ascending trend peaked on day 7. Different concentrations of astragalus root injection improved COLLα1mRNA expression, especially 15% astragalus root injection was the most effective. The type Ⅰ collagen protein expression of 15% and 10% astragalus root injection were significantly greater compared with the control group (P < 0.05). Astragalus root injection improved in vitro cultured osteoblast proliferation and type Ⅰ collagen secretion in a certain dose-effect manner.

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