中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1204-1207.doi: 10.3969/j.issn.1673-8225.2010.07.015

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

血管内皮生长因子在激素性骨坏死过程中的变化特点

武永刚1，段力军1，王坤正2   

  1. 1巴彦淖尔市医院骨科，内蒙古自治区临河市 015000；2西安交通大学第二附属医院，陕西省西安市  710004
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 作者简介:武永刚☆，男，1964年生，汉族，内蒙古自治区临河市人， 2000年西安医科大学毕业，博士，主任医师。

Changing characteristics of vascular endothelial growth factors in glucocorticoid-induced osteonecrosis

Wu Yong-gang1, Duan Li-jun1, Wang Kun-zheng2   

  1. 1 Department of Orthopedics, Bayannaoer Hospital, Linhe   015000, Inner Mongolia Autonomous Region, China; 2 Second Hospital of Xi’an Jiaotong University, Xi’an   710004, Shaanxi Province, China
  • Online:2010-02-12 Published:2010-02-12
  • About author:Wu Yong-gang☆, Doctor, Chief physician, Department of Orthopedics, Bayannaoer Hospital, Linhe 015000, Inner Mongolia Autonomous Region, China

PDF

358

被引次数

4

摘要：

背景：动物实验显示，激素可导致股骨头内毛细血管明显充盈不良，单位面积内毛细血管密度明显下降，软骨下松质骨内微血管数明显减少，但具体机制尚不清楚。
目的：观察激素诱导骨坏死过程中血管内皮生长因子的表达变化。
方法：将中国大耳白兔以抽签法随机分马血清加甲基强的松龙组与单纯甲基强的松龙组。参考Matsui的方法制备典型的兔骨坏死动物模型，马血清加甲基强的松龙组经耳缘静脉注射马血清10 mL/kg，间隔2周后，再次相同剂量及方法注射马血清，间隔2周后腹腔注射甲基强的松龙45 mg/(kg·d)，连续5 d。对照组腹腔注射甲基强的松龙45 mg/(kg·d)，连续5 d。采用RT-RCR技术检测两组用药前、用药后7，14 d，第1次用激素后1，3，7，21，35，49 d骨组织中血管内皮生长因子基因表达，并进行微血管计数。
结果与结论：使用马血清后7 d血管内皮生长因子明显增高，此后呈缓慢回落状态，在应用激素后1 d回落到正常用药前水平，随着应用激素时间的延长，血管内皮生长因子mRNA的表达量逐渐减低，以第7，21天为显著(P < 0.05)，以后逐渐回升，但不能达到正常水平；微血管计数数量逐渐减少，在21 d时减少最明显，与血管内皮生长因子表达量呈正相关。结果提示糖皮质激素抑制了骨组织中血管内皮生长因子的表达，从而抑制了骨内新生血管的形成，使骨组织局部缺血、缺氧状态难以修复重建；血管内皮生长因子的表达与骨组织微血管数量及骨坏死程度密切相关。

关键词: 股骨头坏死, 血管内皮生长因子, 反转录聚合酶链式反应, 动物实验, 中国白兔, 组织工程

Abstract:

BACKGROUND: Animal experiments show that hormone can induce femoral capillaries poor filling, decrease capillaries density in unit area, and reduce capillaries in cancellous bone inferior to cartilage. However, the involved mechanism remains unclear.
OBJECTIVE: To explore the changes of vascular endothelial growth factor (VEGF) during the progress of glucocorticoid induced osteonecrosis.
METHODS: The Chinese white rabbits were randomly divided into horse serum plus prednisone group and prednisone alone group. Typical osteonecrosis model was established by Matsui Method. 10 mL/kg horse serum was injected into horse serum plus prednisone group through the ear margin veins, followed by additional injection after 2 weeks and intraperitoneal injection of prednisone, 45 mg/kg per day for 5 consecutive days. The control group was only subjected to prednisone, 45 mg/kg per day for 5 consecutive days. The VEGF expression was observed by means of reverse transcription polymerase chain reaction (RT-PCR) before, 7, 14 days after administration, 1, 3, 7, 21, 35 and 49 days following the first application of hormone. The capillaries were quantified.
RESULTS AND CONCLUSION: The VEGF was significantly increased 7 days following horse serum, and gradually decreased to levels before treatment 1 days following hormone. The VEGF mRNA expression decreased with increasing hormone treatment, in particular at  7 and 21 days (P < 0.05), but cannot restore to normal level. Microvascular count was decreased gradually, and at 21 days decreased to the minimum, positively correlated with VEGF expression. Results show that adrenal glucocorticoid inhibited VEGF expression in bone tissue and restrained angiogenesis, resulting in ischemia and hypoxia of the local environment in bone tissue. VEGF expression highly correlates with microvascular count and osteonecrosis degree.

中图分类号: