中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 405-409.doi: 10.3969/j.issn.1673-8225.2010.03.007

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

纳米羟基磷灰石/细菌纤维素复合材料及其降解物的细胞相容性评价

郑  琪1,2，奚廷斐 1,2,3，陈艳梅3，王召旭2，万怡灶4，高  川4   

  1. 1温州医学院信息与工程学院，浙江省温州市 325035；2中国药品生物制品检定所医疗器械检测中心，北京市 100050；3北京大学前沿学科交叉研究院，北京市 100871；4天津大学材料科学与工程学院， 天津市 300072
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 奚廷斐，研究员，温州医学院信息与工程学院，浙江省温州市 325035；北京大学前沿学科交叉研究院，北京市 100871； 中国药品生物制品检定所医疗器械检测中心，北京市 100050 Xitingfei@tom.com
  • 作者简介:郑 琪★，女，1982年生，江西省九江市人，汉族，温州医学院在读硕士，主要从事医用生物材料的安全性评价方面的研究。 Flyangle2002@163.com
  • 基金资助:

    国家973计划项目(2007CB936101)，项目名称：纳米纤维仿生支架促进骨再生的机理研究；国家863计划项目(2007AA021901)。

Cytocompatibility of nano-hydroxyapatite/bacterial cellulose nanocomposites and its residues

Zheng Qi 1,2, Xi Ting-fei 1,2,3 , Chen Yan-mei3, Wang Zhao-xu2, Wan Yi-zao4, Gao Chuan4　   

  1. 1 School of Information & Engineering, Wenzhou Medical College, Wenzhou  325035, Zhejiang Province, China; 2 National Institute for Control of Pharmaceutical and Biological Products, Beijing   100050, China; 3 Academy for Advanced Interdisciplinary Studies, Peking University, Beijing   100871, China; 4 School of Materials Science and Engineering, Tianjin University, Tianjin  300072, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Xi Ting-fei, Researcher, School of Information & Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China Xiting-fei@tom.com
  • About author:Zheng Qi★, Studying for master’s degree, School of Information & Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China Flyangle2002@163.com
  • Supported by:

    the National 973 Plan Project of China, No. 2007CB936101; the   National 863 Plan Project of China, No. 2007AA021901

PDF

549

被引次数

28

摘要：

背景：新型复合材料纳米羟基磷灰石/细菌纤维素是一种极具应用前景的骨组织工程材料，而骨组织工程材料要求其本身及其降解产物具有良好的细胞相容性，实验在传统的MTT法评价细胞相容性的基础上，进一步应用流式细胞术的方法从DAN合成周期的角度进行评价。
目的：评价新型纳米复合材料纳米羟基磷灰石/细菌纤维素及其酶降解产物的细胞相容性。
方法：应用体外细胞培养法，观察纳米羟基磷灰石/细菌纤维素复合材料及其降解物对成骨细胞形态学的影响，同时采用 MTT 比色法评价纳米羟基磷灰石/细菌纤维素及其降解物对成骨细胞生长和增殖的影响，并尝试用流式细胞仪检测材料作用于细胞后细胞周期时相的变化，从而在分子水平上评价材料对细胞增殖的影响。
结果与结论：纳米羟基磷灰石/细菌纤维素复合材料及其降解物对成骨细胞的形态无明显影响，对细胞生长和增殖无明显抑制作用。MTT 细胞毒性试验显示原材料及其降解物的细胞增殖率均在80%以上，细胞毒性均为1级，材料对培养细胞无明显细胞毒性。流式细胞仪检测结果显示材料与细胞接触后能降低G0/G1期细胞比例，增加S，G2/M期细胞比例，能增加成骨细胞DNA的合成，促进成骨细胞生长和组织修复。提示纳米羟基磷灰石/细菌纤维素复合材料细胞相容性良好，是一种安全的、很有应用前景的骨组织工程支架材料。

关键词: 细菌纤维素, 羟基磷灰石, 纳米复合材料, 细胞毒性, 细胞周期, 相容性, 成骨细胞

Abstract:

BACKGROUND: The nano-hydroxyapatite/bacterial cellulose (nHAP/BC) nanocomposites has a good prospect of application in bone tissue engineering, and the bone tissue engineered materials and its degradation products should have excellent compatibility. This study further assessed DAN synthesis cycle using flow cytometry on the basis of evaluating cell compatibility by metabolic 3-( 4, 5-dim ethylthiazo l-2-y l) -2, 5-Dipheny l-2H-tetrazolium (MTT) assay.
OBJECTIVE: To evaluate the cytocompatibility of a new-pattern nHAP/BC nanocomposites and its residues. 
METHODS: Effects of nHAP/BC nanocomposites and its residues on morphological changes in osteoblasts were observed using in vitro cell culture method. Effects of nHAP/BC nanocomposites and its residues on osteoblast growth and proliferation were evaluated by MTT assay. Cell cycle phase changes were detected using flow cytometry to evaluate material effects on cell proliferation on molecular levels.
RESULTS AND CONCLUSION: The nHAP/BC nanocomposites and its residues had neither remarkable effects on cell morphology, nor significant inhibition on osteoblast growth and proliferation. Test of MTT cytotoxicity showed that the average cell proliferation rate was over 80% after treated with the material and its residues, with the cytotoxity grade of 1 (non-toxic). Flow cytometry indicated that the rate of G0/G1 was reduced, and the rates of S, G2/M were increased, and the synthesis of DNA was increased, the cellular growth and repair in osteoblasts was accelerated. These indicated that nHAP/BC nanocomposites have good cytocompatibility, and it will be safe and prospected scaffolds in bone tissue engineering.

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