中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 410-414.doi: 10.3969/j.issn.1673-8225.2010.03.008

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

琼脂-羟基磷灰石复合物的仿生合成及细胞相容性

蔡华琼1，李全利1,2，周  健1,2，汤  健3，陈  辉4   

  1. 1安徽医科大学口腔医学院，安徽省合肥市  230032;2安徽医科大学安徽省口腔疾病研究省级实验室，安徽省合肥市 230032;3安徽医科大学附属第一临床医学院，安徽省合肥市  230032; 4中油测井技术服务有限公司，北京市  100000　
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 周 健，硕士，主任医师，教授，博士生导师，安徽医科大学口腔医学院，安徽省合肥市 230032；安徽医科大学安徽省口腔疾病研究省级实验室，安徽省合肥市 230032
  • 作者简介:蔡华琼★，女，1981年生，安徽省巢湖市人，汉族，安徽医科大学口腔医学院在读硕士，主要从事骨组织材料研究。 caihuaqiong@gmail.com

Biomimetic synthesis and cytocompatibility of agar-hydroxyapatite composites

Cai Hua-qiong1, Li Quan-li 1,2, Zhou Jian 1,2, Tang Jian3, Chen Hui4   

  1. 1 College of Stomatology, Anhui Medical University, Hefei  230032, Anhui Province, China; 2 Provincial Laboratory of the Prevention and Treatment for Oral Diseases, Anhui Medical University, Hefei  230032, Anhui Province, China; 3 First Affiliated Clinical College of Anhui Medical University, Hefei  230032, Anhui Province, China; 4 China National Logging Corporation, Beijing  100000, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Zhou Jian, Master, Chief physician, Professor, Doctoral supervisor, College of Stomatology, Anhui Medical University, Hefei 230032, Anhui Province, China; Provincial Laboratory of the Prevention and Treatment for Oral diseases, AnHui Medical University, Hefei 230032, Anhui Province, China
  • About author:Cai Hua-qiong★, Studying for master’s degree, College of Stomatology, Anhui Medical University, Hefei 230032, Anhui Province, China caihuaqiong@gmail.com

PDF

449

被引次数

3

摘要：

背景：以有机大分子作为矿化模版进行骨组织修复材料的仿生构建，是目前骨修复材料的研究热点。而将琼脂应用于骨修复材料的报道较少。
目的：仿生合成一种由琼脂和羟基磷灰石组成的新型纳米复合骨组织修复材料，评价其理化性能和细胞相容性。
方法： ①将一定量的非纳米羟基磷灰石的盐酸溶液，加入一定量的琼脂溶胶中，调整反应体系的pH值至7~8，然后将反应形成的复合物冷冻干燥后即得琼脂-羟基磷灰石复合材料。②将第3代SD大鼠骨髓基质细胞与琼脂-羟基磷灰石复合材料共培养，于培养1，3，5 d时观察细胞生长情况。
结果与结论：X射线衍射仪，傅里叶变换红外光谱仪，热分析仪，透射电镜和扫描电镜对材料进行表征分析，显示琼脂良好控制了磷灰石晶体的生长，纳米的磷灰石晶体均匀分布在琼脂纤维中，琼脂-羟基磷灰石复合物具有多孔结构。共培养3，5 d时骨髓基质细胞在复合物中生长良好，并有较明显的细胞骨架形成。提示琼脂-羟基磷灰石复合物具有良好的理化性质和细胞相容性。

关键词: 琼脂, 羟基磷灰石, 仿生合成, 细胞相容性, 骨修复材料

Abstract:

BACKGROUND: Studies regarding synthesizing composite bone repair materials with organic macromolecules as mineralizers template are the hot research spot. However, reports concerning using agar as bone repair materials are few.
OBJECTIVE: To synthesize a novel agar-hydroxyapatite composite material, and to evaluate its physical and chemical properties as well as cytocompatibility.
METHODS:  ①A certain amount of the non-nano-hydroxyapatite in hydrochloric acid solution was added into a certain amount of agar sol, and the reaction system was adjusted with PH value of 7-8. And then the precipitate was lyophilized to obtain the composite of agar-hydroxyapatite. ②The third generation of rat bone marrow stromal cell (BMSC) was co-cultured with agar-hydroxyapatite. And the growth of cells was observed at days 1, 3 and 5 after culture.
RESULTS AND CONCLUSION: The results of X-ray diffraction patterns, Fourier transform infrared spectrometer (FTIR), thermal analyzer, transmission electron microscope, and scanning electron microscope showed that agar could manipulate the growth of hydroxyapatite, and hydroxyapatite nanocrystals was equably formed on the agar-fibers with high porosity. The BMSC grew well in the composite and form a clear cytoskeletal at days 3 and 5 after culture. The results reveal that agar-hydroxyapatite composite has good physical and chemical properties and cytocompatibility.

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