中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (2): 253-257.doi: 10.3969/j.issn.1673-8225.2010.02.015

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

转化生长因子βⅠ型受体、Ⅰ型胶原在人皮肤增生性瘢痕组织周边区和中心区的表达

耿中利1，董祥林2，马斌林1   

  1. 1 新疆医科大学附属肿瘤医院乳腺、头颈外科，新疆维吾尔自治区乌鲁木齐市 830011；2新疆医科大学第一附属医院整形科，新疆维吾尔族自治区乌鲁木齐市
    830000
  • 出版日期:2010-01-08 发布日期:2010-01-08
  • 通讯作者: 马斌林，教授，主任医师，博士生导师，新疆医科大学附属肿瘤医院乳腺、头颈外科，新疆维吾尔族自治区乌鲁木齐市 830011 mabinlin-doctor@163.com.cn
  • 作者简介:耿中利★，男， 1974年生，安徽省怀远县人，汉族，2006年新疆医科大学毕业，硕士，副主任医师，主要从事乳腺、头颈外科研究。

Expression of type Ⅰ transforming growth factor beta receptor and type Ⅰ collagen in the peripheral and central area of human skin hypertrophic scar

Geng Zhong-li1, Dong Xiang-lin2, Ma Bin-lin1   

  1. 1 Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital, Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China; 2 Department of Plastic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Online:2010-01-08 Published:2010-01-08
  • Contact: Ma Bin-lin, Professor, Chief physician, Doctoral supervisor, Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China mabinlin-doctor@163.com.cn
  • About author:Geng Zhong-li★, Master, Associate chief physician, Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China

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摘要：

背景：近年来国内外学者对转化生长因子β及其受体的研究较多，但转化生长因子β受体1在增生性瘢痕组织周边区分布情况尚未见报道。
目的：比较转化生长因子βⅠ型受体和Ⅰ型胶原在人皮肤增生性瘢痕组织周边区和中心区表达与分布。
方法：收集新疆医科大学第一附属医院整形外科和新疆医科大学附属肿瘤医院乳腺、头颈外科1999/2002住院及门诊各类瘢痕手术患者30例，其中增生性瘢痕20例；非增生性瘢痕10例。取材瘢痕中心区、周边区及正常皮肤组织，每例6块共180块。用免疫组织化学方法检测组织转化生长因子βⅠ型受体、Ⅰ型胶原蛋白含量，对其阳性反应进行定量统计分析。
结果与结论：增生性瘢痕组织转化生长因子βⅠ型受体、Ⅰ型胶原水平明显高于非增生性瘢痕组织和正常皮肤，且免疫阳性反应强。增生性瘢痕周边区转化生长因子βⅠ型受体含量100%，明显高于中心区20%(P < 0.05)；而Ⅰ型胶原中心区与周边区均为100%，差异无显著性意义。非增生性瘢痕周边区、中心区转化生长因子βⅠ型受体、Ⅰ型胶原差异无显著性意义(P > 0.05)；正常皮肤组织转化生长因子βⅠ型受体、Ⅰ型胶原含量极少。结果提示，增生性瘢痕周边区转化生长因子βⅠ型受体表达高于中心区，增生性瘢痕周边区的防治可能是临床防治工作的重点。

关键词: 增生性瘢痕, 转化生长因子&beta, Ⅰ型受体、Ⅰ型胶原, 免疫组织化学, 皮肤组织工程

Abstract:

BACKGROUND: Many studies focus on transforming growth factor β (TGF β) and its receptors, however, the distribution of type Ⅰ TGF receptor (TGF-βR Ⅰ) in peripheral region of hypertrophic scars remain poorly understood. 
OBJECTIVE: To determine the expression and distribution of TGF-βR Ⅰ and type Ⅰ collagen in the peripheral and central areas of human skin hypertrophic scar.
METHODS: A total of 30 cases with human cutaneous scars admitted at the Department of Plastic Surgery, First Affiliated Hospital and Department of Mammary Gland, Head and Neck Surgery, Tumor Hospital of Xinjiang Medical University from 1999 to 2002, were selected, including 20 cases with hypertrophic scar and 10 cases with normal scars. A total of 180 scars were obtained from central and peripheral areas of scars as well as normal skin tissues. The protein contents of TGF-βR1 and type Ⅰ collagen was detected by immunohistochemistry. In addition, the immunostaining positive in these samples was analyzed by semiquantitative analysis.
RESULTS AND CONCLUSION: Compared to non hypertrophic scar and normal skin tissues, the TGF-βR1 expression of hypertrophic scar was obvious greater with strong positive reaction. The TGF-β RⅠ content was 100% in peripheral region of hypertrophic scar, which was notably 20% greater than that of central area (P < 0.05). The content of type Ⅰ collagen was both 100% in peripheral and central areas. The differences of positive TGF-β  RⅠ and type Ⅰ collagen had no significance between peripheral and central areas of non hypertrophic scars (P > 0.05). There were few contents of TGF-β RⅠ and type Ⅰ collagen in normal skin tissues. The expression of TGF-β RⅠ is higher in peripheral than central areas of hypertrophic scar. Therefore, the peripheral area would be emphasized in the clinic work.

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