中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (34): 8953- 8961.doi: 10.12307/2026.850

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

芒果苷缓解骨关节炎疼痛机制：芯片数据、网络药理分析与大鼠模型验证

景  锟1，2，王玉路2，梁  浩1，2，霍宇航1，2，丛龙旭1，2   

  1. 1内蒙古科技大学包头医学院，内蒙古自治区包头市  014040；2内蒙古科技大学包头医学院第一附属医院，内蒙古自治区包头市  014017
  • 收稿日期:2025-09-17 修回日期:2026-01-17 出版日期:2026-12-08 发布日期:2026-04-13
  • 通讯作者: 王玉路，博士，主任医师，内蒙古科技大学包头医学院第一附属医院，内蒙古自治区包头市 014017
  • 作者简介:景锟，男，2000年生，硕士，主要从事创伤骨科方面的研究。
  • 基金资助:
    内蒙古自治区自然科学基金项目(2018MS08142)，项目负责人：王玉路；包头医学院科学研究基金项目(BYJJ-ZRQM202326)，项目负责人：王玉路

Mechanisms by which mangiferin alleviates pain in osteoarthritis: integration of microarray data analysis, network pharmacology, and experimental validation in a rat model

Jing Kun1, 2, Wang Yulu², Liang Hao1, 2, Huo Yuhang1, 2, Cong Longxu1, 2   

  1. 1School of Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, Inner Mongolia Autonomous Region, China; 2The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014017, Inner Mongolia Autonomous Region, China
  • Received:2025-09-17 Revised:2026-01-17 Online:2026-12-08 Published:2026-04-13
  • Contact: Wang Yulu, PhD, Chief physician, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014017, Inner Mongolia Autonomous Region, China
  • About author:Jing Kun, MS, School of Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, Inner Mongolia Autonomous Region, China; The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014017, Inner Mongolia Autonomous Region, China
  • Supported by:
    Natural Science Foundation Project of Inner Mongolia Autonomous Region, No. 2018MS08142 (to WYL); Baotou Medical College Scientific Research Fund Project, No. BYJJ-ZRQM202326 (to WYL) 

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摘要：



文题释义：
芒果苷：是源自芒果树的一种主要生物活性成分，也广泛分布于多种中草药内，属于拥有C-糖基氧杂蒽酮结构的黄色多酚类物质。其结构中的C-葡萄糖基键和多羟基对自由基清除活性有很大贡献，具备抗菌、降胆固醇、抗过敏、强心、抗糖尿病、抗肿瘤、神经防护、抗氧化及免疫调节等多重生物学功能，研究推测芒果苷或许具备预防和治疗骨关节炎疼痛的功效。
骨关节炎：是一种以关节疼痛为主要表现的临床综合征，常伴随不同程度的功能受限与生活质量下降，是关节炎最常见的类型，也是全球范围内疼痛的主要诱因和致残的重要因素之一，最常受影响的外周关节是膝、髋关节和手小关节。

背景：芒果苷作为源自芒果树的主要生物活性成分，广泛存在于多种中草药中，具有抗菌、降胆固醇、抗过敏等多种生物学功能。已有研究提示，芒果苷可能在预防和治疗骨关节炎疼痛方面发挥作用。但截至目前，其具体的作用机制尚不明确。
目的：整合基因表达综合数据库(GEO)芯片数据、网络药理学及分子对接技术，深入剖析芒果苷治疗骨关节炎的关键靶标与潜在机制，并通过动物实验予以验证。
方法：首先，挖掘整合基因表达综合数据库芯片数据以识别骨关节炎的潜在治疗靶标；接下来，对专业数据库进行整合，以此预测芒果苷的作用靶标，同时收集与骨关节炎相关疾病的靶标信息。随后，利用微生信平台绘制维恩图，基于相互作用基因数据库(STRING)构建蛋白质相互作用网络，并开展基因本体和京都基因与基因组百科全书通路富集分析。运用 Cytoscape 3.8.0 软件绘制药物-靶点-通路-疾病网络图，借助蛋白质-配体盲对接工具(CBDOCK2)在线对接平台进行分子对接分析与可视化呈现。采用左膝关节前交叉韧带切断术构建骨关节炎大鼠模型，运用不同浓度的芒果苷对其实施干预，观察并记录药效。
结果与结论：①从多个数据库中挖掘出芒果苷的潜在靶点共计144个；②通过蛋白质相互作用网络分析得到重要靶点有白细胞介素 6、肿瘤坏死因子、核因子κB1等；③京都基因和基因组百科全书通路富集分析结果显示可能涉及脂质和动脉粥样硬化相关通路、晚期糖基化产物-受体、缺氧诱导因子1、雌激素等235条信号通路，与炎症密切相关；④在动物实验中，使用40 μmol/L芒果苷进行为期4周的药物干预后，与假手术组对比，其后肢负重情况无显著差异；⑤提示芒果苷可能凭借多靶点、多通路的作用模式展现骨关节炎的治疗效果，为骨关节炎的治疗提供了新策略及理论支撑。

https://orcid.org/0009-0000-4596-3407(景锟)


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 芒果苷, 骨关节炎, 网络药理学, 疼痛, 基因表达综合数据库

Abstract: BACKGROUND: Mangiferin, a major bioactive compound derived from mango trees, is widely present in various traditional Chinese medicinal herbs and exhibits multiple biological functions including antibacterial, cholesterol-lowering, and anti-allergic effects. Existing studies have suggested that mangiferin may prevent and treat osteoarthritis pain. However, its specific mechanism of action remains unclear to date.
OBJECTIVE: To systematically investigate the key targets and potential mechanisms of mangiferin in the treatment of osteoarthritis by integrating gene expression omnibus (GEO) microarray data analysis, network pharmacology, and molecular docking techniques, and to validate the findings through animal experiments.
METHODS: First, potential therapeutic targets for osteoarthritis were mined using GEO microarray data. Next, the therapeutic targets of mangiferin were predicted by combining professional databases, and disease targets related to osteoarthritis were collected. A Venn diagram was generated using the EVenn platform, and a protein-protein interaction network was constructed using the STRING database. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed. A drug-target-pathway-disease network diagram was generated using Cytoscape 3.8.0 software, and molecular docking analyses and visualizations were conducted via the CBDOCK2 online platform. The osteoarthritis rat model was established using the anterior cruciate ligament transection method, followed by intervention with different concentrations of mangiferin, and the therapeutic effects were observed and recorded.
RESULTS AND CONCLUSION: (1) A total of 144 potential targets for mangiferin were identified from multiple databases. (2) Protein-protein interaction network analysis revealed key targets such as interleukin 6, tumor necrosis factor, and nuclear factor κB1. (3) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that mangiferin may be involved in 235 signaling pathways, including those related to lipids, atherosclerosis, advanced glycation end product-receptor of advanced glycation end product, hypoxia-inducible factor 1, and estrogen, all of which are closely associated with inflammation. (4) In animal experiments, after 4 weeks of treatment with 40 μmol/L mangiferin, there was no significant difference in weight-bearing of the hind limbs between the treatment and sham surgery groups. Overall, mangiferin may exert therapeutic effects on osteoarthritis through a multi-target and multi-pathway mode of action, providing new potential strategies and theoretical foundations for the treatment of osteoarthritis.

Key words: mangiferin, osteoarthritis, network pharmacology, pain, gene expression omnibus database

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