中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (32): 5185-5190.doi: 10.12307/2022.937

• 骨组织构建 bone tissue construction • 上一篇    下一篇

骨疏康治疗去卵巢大鼠骨质疏松的腰椎代谢组学分析

张海永1，黄景文2，谢冰颖2，陈赛楠2，谢丽华2，陈  玄2，李生强2，葛继荣2   

  1. 1福建中医药大学，福建省福州市  350003；2福建省中医药科学院基础研究所，福建省福州市  350003
  • 收稿日期:2022-01-04 接受日期:2022-02-11 出版日期:2022-11-18 发布日期:2022-05-12
  • 通讯作者: 李生强，博士，副研究员，福建省中医药科学院基础研究所，福建省福州市 350003
  • 作者简介:张海永，女，1997年生，河南省淮滨县人，汉族，福建中医药大学在读硕士，主要从事中医药防治骨质疏松症方面的研究。
  • 基金资助:
    国家自然科学基金项目(81774350)，项目负责人：李生强；福建省科技厅省属公益类科研院所基本科研专项(2019R1003-1)，项目负责人：李生强

Anti-osteoporosis effect of Gushukang in ovariectomized rats: a lumbar metabonomic analysis

Zhang Haiyong1, Huang Jingwen2, Xie Bingying2, Chen Sainan2, Xie Lihua2, Chen Xuan2, Li Shengqiang2, Ge Jirong2   

  1. 1Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China; 2Basic Research Institute, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, Fujian Province, China
  • Received:2022-01-04 Accepted:2022-02-11 Online:2022-11-18 Published:2022-05-12
  • Contact: Li Shengqiang, MD, Associate researcher, Basic Research Institute, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, Fujian Province, China
  • About author:Zhang Haiyong, Master candidate, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81774350 (to LSQ); the Fundamental Scientific Research Project of Fujian Provincial Public Welfare Scientific Research Institutes of Fujian Provincial Department of Science and Technology, No. 2019R1003-1 (to LSQ)

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摘要：

文题释义：
代谢组学：是在后基因组学时代新近发展的一门学科，其主要目标是定量研究生命体对外界刺激、病理生理变化以及本身基因突变而产生的其体内代谢物水平的多元动态反应。
液相色谱-质谱联用：色谱-质谱技术成为代谢组学研究最重要的工具，是以高效液相色谱作为分离系统，高分辨率质谱为检测系统的一种串联分析平台；与其他色谱-质谱联用技术相比，液相色谱-质谱联用更适用于分析难挥发或热稳定性差的代谢物。

背景：骨疏康对骨质疏松症的具体分子作用机制和治疗靶点研究不足，需进一步研究探讨。
目的：为进一步探索骨疏康治疗骨质疏松症的具体机制及作用靶点，利用超高效液相色谱-四级杆-飞行时间串联质谱技术的非靶向代谢组学方法，分析鉴定去卵巢大鼠腰椎的代谢物变化，探讨骨疏康对去卵巢大鼠骨质疏松症的治疗作用和潜在机制。
方法：选择雌性SD大鼠，随机分为模型组、骨疏康组及假手术组，前两组采用去卵巢法建立骨质疏松症模型。造模4周后，骨疏康组大鼠给予骨疏康灌胃；假手术组和模型组分别给予等体积生理盐水灌胃。连续灌胃12周后利用超高效液相色谱-四级杆-飞行时间串联质谱技术进行大鼠腰椎代谢物分析，筛选出共同差异代谢物，对其进行京都基因与基因组百科全书通路等生物信息学分析。
结果与结论：①3组共筛选出24种共同显著性差异代谢物；②京都基因与基因组百科全书富集分析表明，骨疏康可能调节脂肪酸代谢、甘油磷脂代谢、氨基酸代谢等重要通路参与保护骨质疏松模型大鼠的骨代谢过程；③结果显示，骨疏康可能通过干预脂质代谢和氨基酸代谢发挥其抗骨质疏松作用，从而达到预防骨质疏松症的目的。
缩略语：京都基因与基因组百科全书：Kyoto Encyclopedia of Genes and Genomes，KEGG

https://orcid.org/0000-0003-0795-749X (张海永) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 骨质疏松, 代谢组学, 骨疏康, 脂质代谢, 氨基酸代谢, 差异代谢物, 质谱, 中医药

Abstract: BACKGROUND: The research on the specific molecular mechanisms and therapeutic targets of Gushukang on osteoporosis is insufficient, which needs further research and discussion.
OBJECTIVE: To analyze and identify the changes of lumbar metabolites in ovariectomized rats, explore the therapeutic effect of Gushukang on osteoporosis in ovariectomized rats and discuss its potential mechanism by using the non-targeted metabonomics of ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass, thereby to further explore the specific mechanisms and targets of Gushukang in the treatment of osteoporosis.
METHODS: Female Sprague-Dawley rats, which were ovariectomized to establish osteoporosis model, were divided into model group, Gushukang group, and sham operation group. Osteoporotic models were established using ovariectomy. Four weeks after modeling, rats in the Gushukang group were given Gushukang by gavage; and those in the sham operation and model groups were given the equal volume of normal saline. After 12 weeks of continuous gavage, the lumbar metabolites of rats were analyzed by the ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass technology, and the common differential metabolites were selected for bioinformatics analysis using Kyoto Encyclopedia of Genes and Genomes pathway. 
RESULTS AND CONCLUSION: A total of 24 common significant differential metabolites were identified in the three groups. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that Gushukang could regulate important pathways such as fatty acid metabolism, glycerol phospholipid metabolism and amino acid metabolism, and participate in the protection of bone metabolism in osteoporosis model rats. These findings indicate that Gushukang exerts its anti-osteoporosis effect by interfering with lipid metabolism and amino acid metabolism, so as to achieve the purpose of preventing osteoporosis.

Key words: osteoporosis, metabonomics, Gushukang, lipid metabolism, amino acid metabolism, differential metabolites, mass spectrum, traditional Chinese medicine

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