中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (25): 4058-4063.doi: 10.12307/2022.414
• 干细胞综述 stem cell review • 上一篇 下一篇
张高飞,王 迪,李佳美,娄涵潇,曾跃勤,刘文军
收稿日期:
2021-03-12
接受日期:
2021-05-28
出版日期:
2022-09-08
发布日期:
2022-01-26
通讯作者:
刘文军,博士,副教授,昆明医科大学第二附属医院烧伤科,云南省昆明市 650188
作者简介:
张高飞,女,1993年生,河南省洛阳市人,汉族,2021年昆明医科大学第二附属医院毕业,硕士,主要从事烧伤整形方面的研究。
基金资助:
Zhang Gaofei, Wang Di, Li Jiamei, Lou Hanxiao, Zeng Yueqin, Liu Wenjun
Received:
2021-03-12
Accepted:
2021-05-28
Online:
2022-09-08
Published:
2022-01-26
Contact:
Liu Wenjun, MD, Associate professor, Department of Burns, Second Affiliated Hospital of Kunming Medical University, Kunming 650188, Yunnan Province, China
About author:
Zhang Gaofei, Master, Department of Burns, Second Affiliated Hospital of Kunming Medical University, Kunming 650188, Yunnan Province, China
Supported by:
摘要:
文题释义:
细胞自噬:是细胞在应激状态下通过膜结构将受损细胞器或蛋白质运送到溶酶体或自噬溶酶体,利用多种溶菌酶降解受损的物质,从而达到细胞新陈代谢和细胞器更新的过程。自噬参与细胞以及生物体的发育和分化的全过程,与细胞增殖以及损伤后修复等密切相关。
间充质干细胞:是一种能够进行自我更新并具有多向分化功能的成体干细胞,其可在体外实现轻松扩增,并能够选择性地迁移到受损组织附近分泌相应营养因子来修复组织缺损。
背景:近年来,间充质干细胞在创面愈合中的作用受到广泛关注。尽管间充质干细胞可以促进伤口愈合,但其作用机制并不完全十分清楚。
目的:对近年来间充质干细胞促进创面愈合的作用及其机制作一综述。
方法:以“间充质干细胞,创面愈合,自噬,干细胞”“mesenchymal stem cells,wound healing,autophagy,stem cells”为关键词,检索自建库以来至2021年3月在PubMed、Cochrane library、中国知网、维普中文科技期刊全文数据库、万方医学网、中国生物医学文献数据库等收录的相关文献。
结果与结论:间充质干细胞移植创面后,可通过调控自噬效应与创面环境互为影响,协调统一,共同促进创面组织的愈合。同时,间充质干细胞可作为理想的自噬诱导体,促进创面成纤维细胞的增殖和迁移,抑制炎症反应,促进内皮细胞的血管生成从而促进受损创面的愈合。然而,目前尚不清楚间充质干细胞对自噬活性的作用机制。因此,间充质干细胞植入创面中引起的自噬消除及相关分子机制成为当前创面愈合研究的重点。
https://orcid.org/0000-0001-7400-7385 (刘文军)
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
中图分类号:
张高飞, 王 迪, 李佳美, 娄涵潇, 曾跃勤, 刘文军. 间充质干细胞通过调控自噬促进创面愈合[J]. 中国组织工程研究, 2022, 26(25): 4058-4063.
Zhang Gaofei, Wang Di, Li Jiamei, Lou Hanxiao, Zeng Yueqin, Liu Wenjun. Mesenchymal stem cells promote wound healing by regulating the autophagy[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(25): 4058-4063.
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1.1 文献检索和筛选要求 以“间充质干细胞,创面愈合,自噬,干细胞”“mesenchymal stem cells,wound healing,autophagy,stem cells”为关键词,检索自建库至 2021年3月收录在 PubMed、Cochrane library、中国知网、维普中文科技期刊全文数据库、万方医学网、中国生物医学文献数据库的相关文献。共检索到中英文文献 4 607 篇,其中与烧伤创面发展相关文献1 492 篇,与干细胞相关文献1 486篇,与自噬相关文献1 629篇。排除与研究目的不符及内容重复和观点不新颖的文献,纳入121篇符合要求的文献进行综述,见图1。
1.2 文献筛选标准
纳入标准:与间充质干细胞治疗烧伤创面相关的研究,以及入选文献中的参考文献。
排除标准:与研究目的无关、内容重复或观点不新颖的文献。
1.3 质量评估及数据的提取 计算机初检得到4 607篇文献,经资料收集者互相评估纳入文献的有效性和适用性,通过阅读文题和摘要进行初步筛选;排除中英文文献重复性研究,以及内容不相关的文献,最后纳入121篇文献进行综述,其中17篇与烧伤创面发展及其机制相关,14篇与干细胞作为组织工程种子细胞的优势相关,28篇与自噬的分类和作用机制相关,16篇与干细胞自噬相关,31篇与创面自噬相关,15篇与间充质干细胞的自噬效应促进创面成纤维细胞增殖以及抑制炎症反应和促进血管再生等有关。
文题释义:
细胞自噬:是细胞在应激状态下通过膜结构将受损细胞器或蛋白质运送到溶酶体或自噬溶酶体,利用多种溶菌酶降解受损的物质,从而达到细胞新陈代谢和细胞器更新的过程。自噬参与细胞以及生物体的发育和分化的全过程,与细胞增殖以及损伤后修复等密切相关。
间充质干细胞:是一种能够进行自我更新并具有多向分化功能的成体干细胞,其可在体外实现轻松扩增,并能够选择性地迁移到受损组织附近分泌相应营养因子来修复组织缺损。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
皮肤组织的结构完整性常因烧伤、烫伤等因素而被损害,特别是大面积损伤的皮肤创面,如果不能及时有效的进行修复,可能会引起内环境紊乱,甚至导致局部和全身器官功能障碍。近年来研究发现,间充质干细胞作为医用组织工程的新型种子细胞,能促进创面愈合,但目前关于间充质干细胞促进创面修复的机制仍不十分清楚。随着研究的深入,自噬在间充质干细胞的作用日趋明显,可能为临床创面愈合提供新的治疗思路。相信在不久的将来科学家会将自噬与间充质干细胞以及创面愈合之间的关系阐述清楚,为临床创面愈合提供新的思路和方法。
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
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